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The map label for this gene is gcvT

Identifier: 49187128

GI number: 49187128

Start: 4051356

End: 4052456

Strand: Reverse

Name: gcvT

Synonym: BAS4131

Alternate gene names: 49187128

Gene position: 4052456-4051356 (Counterclockwise)

Preceding gene: 49187131

Following gene: 49187127

Centisome position: 77.5

GC content: 37.6

Gene sequence:

>1101_bases
ATGATTACATTACAACGTACACCGTTATTTGATGTATACGCGAAGTATGGTGGGAAAACAATCGACTTCGGTGGTTGGGA
GTTACCAGTTCAATTTTCAAGCATTAAAGAAGAACATGAAGCTGTACGTACAGCGGCGGGTTTGTTCGATGTGTCTCATA
TGGGAGAAGTTGAGGTAAAAGGTGTAGATAGTTTAGCATTTTTACAACGTGTTGTTACAAATGACGTATCTACCTTAAAG
GTAGGAGGCGCACAATATACAGCAATGTGCTACGAAAATGGTGGTACAGTAGATGATTTATTAATCTACAAACGTGGTGA
AGAAGACTATTTATTAGTTATCAACGCATCAAATATCGAGAAAGATTACGAATGGTTAGCTAGCCATGTAATTGGCGATG
CGACAGTAGTCAATGTTTCTAGCGAAGTTGCACAGCTTGCAATTCAAGGACCAAAAGCAGAAGGCATTTTACAAAAAGTT
GTGTCAGAAGATTTAAAAGAAATTAAGTTCTTTAAATTTAAAAACGATATTCTTGTAGATGGAATTCCAGCACTTGTATC
TCGTACAGGTTACACAGGTGAAGATGGATTTGAAATTTACTGTAAGAGTGAAGATGCAGCAAAACTTTGGGAGAAACTTC
TTGAAGTTGGAGCAGAAGAAGGCTTAAAAGCATGTGGTTTAGGTGCTCGTGATACACTTCGCTTCGAAGCAACATTACCA
CTTTATGGTCAAGAATTATCAAAAGATATTACACCGATTGAAGCTGGAATTGGCTTTGCGGTAAAAACAAATAAAGAAGC
AGACTTCTTTGGAAAAGCAACGTTAAAAGAGCAAAAAGAAAACGGTGCGCCTCGCAAATTAGTCGGCATCGAAGTAATTG
AACGTGGAATTCCTCGTACGCATTACCCTGTATTTATTGGAGAAGAAAAGATCGGGGAAGTAACAAGTGGTACACAATCT
CCAACGTTAAAGAAAAGCATTGGTTTAGCACTAATTGATGTAAAATACGCAGCAGTTGATACAGAAGTAGAAATTGAAAT
TCGTAATAAACGCGTCAAAGCAGTAGTTGTTCCAACACCATTTTATAAACGTTCAAAGTAA

Upstream 100 bases:

>100_bases
AATATAATGAGATTCCGTATATCCTATCTTTTTAGAATGAAAATGTTTAGAAAAGAGTAACAACTTTTATTGATTTGTCA
GAGCATAAGGGGGAGAGATC

Downstream 100 bases:

>100_bases
CGGGGAGAGGGGTAGATTTCATGTTGCATCGTTATCTTCCAATGACAGAAGAAGACAAAAAAGAAATGTTACAAACGATC
GGCGTTCAAACGATCGATGA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVKGVDSLAFLQRVVTNDVSTLK
VGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIEKDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKV
VSEDLKEIKFFKFKNDILVDGIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLP
LYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRTHYPVFIGEEKIGEVTSGTQS
PTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTPFYKRSK

Sequences:

>Translated_366_residues
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVKGVDSLAFLQRVVTNDVSTLK
VGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIEKDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKV
VSEDLKEIKFFKFKNDILVDGIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLP
LYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRTHYPVFIGEEKIGEVTSGTQS
PTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTPFYKRSK
>Mature_366_residues
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVKGVDSLAFLQRVVTNDVSTLK
VGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIEKDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKV
VSEDLKEIKFFKFKNDILVDGIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLP
LYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRTHYPVFIGEEKIGEVTSGTQS
PTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTPFYKRSK

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=370, Percent_Identity=34.5945945945946, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI257796258, Length=356, Percent_Identity=33.9887640449438, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI24797151, Length=336, Percent_Identity=30.6547619047619, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI257796254, Length=363, Percent_Identity=32.5068870523416, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI257796256, Length=318, Percent_Identity=32.3899371069182, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI197927446, Length=308, Percent_Identity=26.2987012987013, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI21361378, Length=308, Percent_Identity=26.2987012987013, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI194306651, Length=317, Percent_Identity=25.2365930599369, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789272, Length=373, Percent_Identity=36.4611260053619, Blast_Score=238, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17560118, Length=371, Percent_Identity=36.3881401617251, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71994045, Length=374, Percent_Identity=25.9358288770053, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994052, Length=374, Percent_Identity=25.9358288770053, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32563613, Length=341, Percent_Identity=26.3929618768328, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320222, Length=389, Percent_Identity=35.2185089974293, Blast_Score=206, Evalue=3e-54,
Organism=Drosophila melanogaster, GI20129441, Length=375, Percent_Identity=35.2, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28571104, Length=272, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20130091, Length=367, Percent_Identity=24.2506811989101, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_BACAA (C3P8D5)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868518.1
- ProteinModelPortal:   C3P8D5
- SMR:   C3P8D5
- EnsemblBacteria:   EBBACT00000129700
- GeneID:   7848034
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4467
- GeneTree:   EBGT00050000001932
- ProtClustDB:   PRK00389
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40225; Mature: 40225

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVK
CEEECCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GVDSLAFLQRVVTNDVSTLKVGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIE
CCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCC
KDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKVVSEDLKEIKFFKFKNDILVD
HHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEC
GIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLP
CHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCHHCCHHHCCCCCCCEEEEEEECC
LYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRT
CCHHHHCCCCCCHHHCCEEEEECCCCCCHHCCHHHHHHHHCCCCCEEEHHHHHHCCCCCC
HYPVFIGEEKIGEVTSGTQSPTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTP
CCCEEECCHHHCCCCCCCCCCCHHHHCCEEEEEEEEEEECCEEEEEEECCEEEEEEECCC
FYKRSK
CCCCCC
>Mature Secondary Structure
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVK
CEEECCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GVDSLAFLQRVVTNDVSTLKVGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIE
CCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCC
KDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKVVSEDLKEIKFFKFKNDILVD
HHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEC
GIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLP
CHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCHHCCHHHCCCCCCCEEEEEEECC
LYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRT
CCHHHHCCCCCCHHHCCEEEEECCCCCCHHCCHHHHHHHHCCCCCEEEHHHHHHCCCCCC
HYPVFIGEEKIGEVTSGTQSPTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTP
CCCEEECCHHHCCCCCCCCCCCHHHHCCEEEEEEEEEEECCEEEEEEECCEEEEEEECCC
FYKRSK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA