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The map label for this gene is folD

Identifier: 49187083

GI number: 49187083

Start: 4020080

End: 4020940

Strand: Reverse

Name: folD

Synonym: BAS4085

Alternate gene names: 49187083

Gene position: 4020940-4020080 (Counterclockwise)

Preceding gene: 49187084

Following gene: 49187082

Centisome position: 76.9

GC content: 37.75

Gene sequence:

>861_bases
ATGGTAGCAGTAATCATCAAAGGAAATGAAGTTGCGGAGAAAAAACGAGCACAATTAAAAGAAGAAGTTGTGAAGTTGAA
AGAGCAAGGGATTGTACCAGGATTAGCAGTTATTTTAGTTGGAGAAGATCCAGCATCTCGTTCTTATGTAAAAGGAAAAG
AAAAGGGCTGTGAACAAGTAGGAATCTATTCAGAGCTAATTGAATTTCCTGAAACAATTACGGAGGAGCGTTTGCTTGCT
GAAATCGATCGCTTAAATGGCGATGACCGTATTAATGGCATATTAGTGCAATTACCTTTACCAAAACATATTGAAGAAAA
AGCTATCATTGAAAGAATTTCACCAGAAAAAGATGTAGATGGATTTCACCCAATCAGCGTGGGACGTATGATGACGGGAC
AAGATACATTCCTTCCATGTACACCGCATGGTATTGTAGAGTTAGTAAAAGAAACAAATCTTGATATCTCAGGAAAACAT
GTTGTAGTAATTGGAAGAAGTAATATTGTCGGCAAGCCAGTGGGACAATTGTTCTTAAATGAAAATGCAACTGTCACATA
CTGTCATTCTAAGACGCAAAACATGAAAGAGTTAACGAAGTTAGCTGATATTTTAATCGTAGCAGTAGGACGACCTAAAA
TGGTAACAGCTGATTATATCAAAGAAGGAGCAGTTGTAATCGACGTTGGTGTAAACCGTTTAGAAACAGGAAAACTTTGT
GGTGATGTTGACTTTGACAACGTGTTAGATGTTGCAAGTTACATTACACCTGTACCAAAGGGTGTTGGTCCAATGACAAT
CACGATGCTTCTTCATAATACTGTTGAATCGGCTAAGCGTGCAGGTGTCGTTTGTAAATAA

Upstream 100 bases:

>100_bases
TTGCTAAAACATTTGGGGATGAGGAATCTCGTCGTTTTATTAACGGCGTTTTATCTAATATAAAAGATACACTGTAATTT
CTTTAAGGGGGAATCTTAAA

Downstream 100 bases:

>100_bases
TTTGAGTCGGCTATGAGGAGAGAGAAATGGAGAAACAATATTTAACCGTTACAGCGTTAACACGCTATATAAAAACAAAA
ATAGAATATGATCCGCATTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVASYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK

Sequences:

>Translated_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVASYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
>Mature_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVASYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=47.2413793103448, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=49.3197278911565, Blast_Score=250, Evalue=9e-67,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=46.9594594594595, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=23.5807860262009, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=50.3597122302158, Blast_Score=277, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=46.2328767123288, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=45.6747404844291, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.9463087248322, Blast_Score=232, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=27.8996865203762, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.0207612456747, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.0207612456747, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.0207612456747, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.0207612456747, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=43.0508474576271, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=43.0508474576271, Blast_Score=231, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BACAA (C3P7W0)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868475.1
- ProteinModelPortal:   C3P7W0
- SMR:   C3P7W0
- EnsemblBacteria:   EBBACT00000124778
- GeneID:   7851440
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4422
- GeneTree:   EBGT00050000002737
- ProtClustDB:   PRK14190
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31182; Mature: 31182

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCHHHCHHHH
GIYSELIEFPETITEERLLAEIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFDNVLDVASYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCHHHCHHHH
GIYSELIEFPETITEERLLAEIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFDNVLDVASYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA