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The map label for this gene is ribE [H]

Identifier: 49187017

GI number: 49187017

Start: 3955934

End: 3956578

Strand: Direct

Name: ribE [H]

Synonym: BAS4019

Alternate gene names: 49187017

Gene position: 3955934-3956578 (Clockwise)

Preceding gene: 49187016

Following gene: 49187018

Centisome position: 75.66

GC content: 36.9

Gene sequence:

>645_bases
ATGTTTACAGGAATTGTAGAAGAATTAGGAACGATAACAAATATGCAACAAAGCGGAGAAGCAATGAAGTTAACGATACA
TGCCAATAAAATTTTATCAGATGTTCACTTAGGTGATAGCATCGCGGTTAACGGTATTTGCTTAACTGTAACGAGTTTCA
CAACAACTTCATTCACAGTGGATGCAATGCCCGAAACAATGAAATCAACATCACTTCGCCTACTCAAATCACACTCTAAA
GTAAATTTAGAGCGAGCAATGGCTGCAAATGGACGATTCGGTGGACATTTCGTTTCAGGACATATTGATGGCATCGGGAC
GATATTAAACAAAAAACAACACTATAATGCCATCTATTACAAAATAGCTATTTCTGATGAACTGCTGCGCTATTGTTTGC
ATAAAGGATCCATTGCTGTTGATGGAACGAGCTTAACAATATTTGATATAGACGAATCTTCCATTACAATTTCACTCATC
CCGCACACAGTAAGTGAATCTGTTATCGGAGAAAAGAATGCCGGTGACATCGTAAACATTGAGTGTGACATGATTGGTAA
ATATATCGAACGCTTTATTACTAAGCCTGTAAAACGAACAGGTTCAATGACCGAAAACTTTTTACAAGAAAACGGATTTC
TATAA

Upstream 100 bases:

>100_bases
TTTCAACATTACAAGATGCGCTTTCCTTGGAAATTCAAGAGATGAAACAAATTGGTGATGATATAAAAATCGTTGCGCAT
GCCCGAAACGAGGTGACGAA

Downstream 100 bases:

>100_bases
GGGGGAAGCATTATGTTTCATCGTATTGAAGAAGCTCTAGAAGATTTAAAACAAGGAAAAGTCGTTATCGTATGTGATGA
TGAAAACCGTGAAAACGAAG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MFTGIVEELGTITNMQQSGEAMKLTIHANKILSDVHLGDSIAVNGICLTVTSFTTTSFTVDAMPETMKSTSLRLLKSHSK
VNLERAMAANGRFGGHFVSGHIDGIGTILNKKQHYNAIYYKIAISDELLRYCLHKGSIAVDGTSLTIFDIDESSITISLI
PHTVSESVIGEKNAGDIVNIECDMIGKYIERFITKPVKRTGSMTENFLQENGFL

Sequences:

>Translated_214_residues
MFTGIVEELGTITNMQQSGEAMKLTIHANKILSDVHLGDSIAVNGICLTVTSFTTTSFTVDAMPETMKSTSLRLLKSHSK
VNLERAMAANGRFGGHFVSGHIDGIGTILNKKQHYNAIYYKIAISDELLRYCLHKGSIAVDGTSLTIFDIDESSITISLI
PHTVSESVIGEKNAGDIVNIECDMIGKYIERFITKPVKRTGSMTENFLQENGFL
>Mature_214_residues
MFTGIVEELGTITNMQQSGEAMKLTIHANKILSDVHLGDSIAVNGICLTVTSFTTTSFTVDAMPETMKSTSLRLLKSHSK
VNLERAMAANGRFGGHFVSGHIDGIGTILNKKQHYNAIYYKIAISDELLRYCLHKGSIAVDGTSLTIFDIDESSITISLI
PHTVSESVIGEKNAGDIVNIECDMIGKYIERFITKPVKRTGSMTENFLQENGFL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=216, Percent_Identity=32.4074074074074, Blast_Score=106, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=43.4146341463415, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23421; Mature: 23421

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEELGTITNMQQSGEAMKLTIHANKILSDVHLGDSIAVNGICLTVTSFTTTSFTV
CCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCCCEEECEEEEEEEEEEEEEEEE
DAMPETMKSTSLRLLKSHSKVNLERAMAANGRFGGHFVSGHIDGIGTILNKKQHYNAIYY
ECCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEE
KIAISDELLRYCLHKGSIAVDGTSLTIFDIDESSITISLIPHTVSESVIGEKNAGDIVNI
EEEECHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEEECCHHHHHHCCCCCCCCEEEE
ECDMIGKYIERFITKPVKRTGSMTENFLQENGFL
EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVEELGTITNMQQSGEAMKLTIHANKILSDVHLGDSIAVNGICLTVTSFTTTSFTV
CCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCCCEEECEEEEEEEEEEEEEEEE
DAMPETMKSTSLRLLKSHSKVNLERAMAANGRFGGHFVSGHIDGIGTILNKKQHYNAIYY
ECCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEE
KIAISDELLRYCLHKGSIAVDGTSLTIFDIDESSITISLIPHTVSESVIGEKNAGDIVNI
EEEECHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEEECCHHHHHHCCCCCCCCEEEE
ECDMIGKYIERFITKPVKRTGSMTENFLQENGFL
EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709 [H]