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The map label for this gene is ptsH

Identifier: 49186957

GI number: 49186957

Start: 3904015

End: 3904278

Strand: Reverse

Name: ptsH

Synonym: BAS3959

Alternate gene names: 49186957

Gene position: 3904278-3904015 (Counterclockwise)

Preceding gene: 49186958

Following gene: 49186956

Centisome position: 74.67

GC content: 36.36

Gene sequence:

>264_bases
ATGGAAAAAATCTTTAAAGTAACTAGCGACTCAGGAATTCATGCTCGTCCAGCAACTCTACTTGTAAACACTGCAAGCAA
ATTTGGTTCTGACATTAACTTAGAGTATAACGGAAAGAACGTTAACTTAAAATCAATCATGGGCGTTATGTCTTTAGGCA
TTCAACAAAACGCAGAAATTAAAATCACTGCAAATGGTGATGATGCAGCTCAAGCACTAGCAGCTATCGAAGAAACTATG
AAAAACGAAGGATTAGGAGAATAA

Upstream 100 bases:

>100_bases
CGATGTGGTTGACCGAACATCGCCTATTATATACAATAGATGATGTAGTACTCACGTCTATACAACTAAAAAAACTGTAA
TTTAAAGGAGATAAATTATC

Downstream 100 bases:

>100_bases
TGACTCTTAACATTCAAGGGATCGCTGCATCAAGTGGGATTGCTATTGCAAAGGCTTTCAGACTTGAAAATCCTGAATTT
AACATCGAACAGAAATCAAT

Product: phosphocarrier protein HPr

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGSDINLEYNGKNVNLKSIMGVMSLGIQQNAEIKITANGDDAAQALAAIEETM
KNEGLGE

Sequences:

>Translated_87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGSDINLEYNGKNVNLKSIMGVMSLGIQQNAEIKITANGDDAAQALAAIEETM
KNEGLGE
>Mature_87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGSDINLEYNGKNVNLKSIMGVMSLGIQQNAEIKITANGDDAAQALAAIEETM
KNEGLGE

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1788494, Length=86, Percent_Identity=46.5116279069767, Blast_Score=73, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTHP_BACTI (Q9F166)

Other databases:

- EMBL:   AF117385
- HSSP:   P42013
- ProteinModelPortal:   Q9F166
- SMR:   Q9F166
- PhosSite:   Q9F166
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9239; Mature: 9239

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIFKVTSDSGIHARPATLLVNTASKFGSDINLEYNGKNVNLKSIMGVMSLGIQQNAEI
CCCEEEECCCCCCCCCCCEEEEECHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEE
KITANGDDAAQALAAIEETMKNEGLGE
EEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKIFKVTSDSGIHARPATLLVNTASKFGSDINLEYNGKNVNLKSIMGVMSLGIQQNAEI
CCCEEEECCCCCCCCCCCEEEEECHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEE
KITANGDDAAQALAAIEETMKNEGLGE
EEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11168390