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The map label for this gene is ptsI [H]

Identifier: 49186956

GI number: 49186956

Start: 3902303

End: 3904015

Strand: Reverse

Name: ptsI [H]

Synonym: BAS3958

Alternate gene names: 49186956

Gene position: 3904015-3902303 (Counterclockwise)

Preceding gene: 49186957

Following gene: 49186952

Centisome position: 74.67

GC content: 38.18

Gene sequence:

>1713_bases
ATGACTCTTAACATTCAAGGGATCGCTGCATCAAGTGGGATTGCTATTGCAAAGGCTTTCAGACTTGAAAATCCTGAATT
TAACATCGAACAGAAATCAATTACAAACGAAGCTGCAGAAATTGCACGCTTAGAAGCTGCGCTTGAGAAAGCAAAAACTG
AATTAGAAGCTATTAAGGACCACGCTTTTGCTGAGCTAGGTGCTGACAAAGCTGCGATCTTTGAAGCACATTTATTAGTG
TTAAATGATCCAGAACTAGTAAACCCAGTAAAAGATAAAGTAAATAGCGAAAAAGTAAATGCTGAATTTGCAATGGATGA
AGTTGCATCAATGTTTATCTCTATGTTTGAAAACATGGATAACGAATATATGAAAGAACGTGCTGCGGACATTCGTGACG
TAACAAAACGTGTTCTTGCGCATTTACTAGGCATTAACTTCTCAAATCCTGGTACAAATTCTGAAGAAGTAATCATTATT
GCTGAAGATTTAACACCATCTGATACAGCTCAGTTAAACCGTAAGTATGCAAAAGGTTTTACTACCGATATCGGCGGACG
TACATCTCACTCTGCAATTATGGCTCGCTCTATGGAAATTCCAGCTGTTGTTGGTACAAAAGTTGTTATGGAGAAAATCC
AAAACGGCGATATCGTAATCATCGATGGTTTAGATGGGGAAGTAATTGTTAATCCATCAGAAGAAACTCTTCGCTCGTTT
GAAGAAAAGAAAGCGAAATTTGAAGAGCAAAAAGCTGAATGGGCAAAATTAAAAGACCAAGCTACTGTAACAAGTGATGG
ACATCACGTTGAGCTTGTTGCAAATATCGGAACACTAAATGATGTACAAGGTATTATCGATAATGGCGGAGAAGGCGTTG
GTTTATACCGTACAGAATTCTTATACATGGGCCGTGACAATCTTCCAACAGAAGAAGAGCAGTTCGAAGCGTATAAAGCA
GTTCTTGAAGGTGTAAAAGAAGGTCAACCTGTTGTTGTTCGTACACTTGACATCGGTGGAGATAAAGAGCTTCCATACTT
ACATTTACCAAAAGAAATGAACCCATTCTTAGGCTACCGTGCAATTCGCTTATGTCTTGATGAGCAAGATGTGTTCCGTA
CACAACTTCGTGCATTACTTCGTGCTAGCGTATACGGTAACTTAAAAATTATGTTCCCAATGATTGCAACTCTTGATGAG
TTCCGTCAAGCAAAAGCGATCTTATTAGAAGAAAAAGCGAAACTTGTAGAAGTGGGTACAACTGTTTCTGATTCTATTGA
AGTTGGTATGATGGTTGAAATCCCAGCTTCAGCAGTATTAGCAGATCAATTCGCAAAAGAAGTTGATTTCTTCTCTATCG
GAACAAATGACTTAATTCAATACACAATGGCTGCAGACCGTATGAACGAACAAGTATCTTACTTATACCAACCATATAAC
CTATCTATTTTACGTCTTGTAAAAATGGTTATCGATGCTGCTCATAAAGAAGGCAAATGGGCTGGTATGTGTGGTGAGAT
GGCGGGCGATTCACTTGCTATCCCATTATTATTAGGATTAGGTTTAGATGAGTTCAGTATGAGTGCAACATCTATTCTTC
CTGCAAGAACACAACTAAGCAAGTTGTCAAAAGCAGAAATGGAAACATTAGCAGAAAAAGCATTAATGATGTCAACTGCT
GAAGAAGTTGTTGAACTAGTTAAAAGCATATAA

Upstream 100 bases:

>100_bases
AACAAAACGCAGAAATTAAAATCACTGCAAATGGTGATGATGCAGCTCAAGCACTAGCAGCTATCGAAGAAACTATGAAA
AACGAAGGATTAGGAGAATA

Downstream 100 bases:

>100_bases
TTAATAAAAAAACCTGAGTCGATTTGAAATCGGTCTCAGGTTTTTTCTATGAGAAAAGTAAAACTTATTTTCTTACTCTT
GAAGATAAGGAACAGAATTA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTLNIQGIAASSGIAIAKAFRLENPEFNIEQKSITNEAAEIARLEAALEKAKTELEAIKDHAFAELGADKAAIFEAHLLV
LNDPELVNPVKDKVNSEKVNAEFAMDEVASMFISMFENMDNEYMKERAADIRDVTKRVLAHLLGINFSNPGTNSEEVIII
AEDLTPSDTAQLNRKYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVVMEKIQNGDIVIIDGLDGEVIVNPSEETLRSF
EEKKAKFEEQKAEWAKLKDQATVTSDGHHVELVANIGTLNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKA
VLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLDEQDVFRTQLRALLRASVYGNLKIMFPMIATLDE
FRQAKAILLEEKAKLVEVGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYN
LSILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMETLAEKALMMSTA
EEVVELVKSI

Sequences:

>Translated_570_residues
MTLNIQGIAASSGIAIAKAFRLENPEFNIEQKSITNEAAEIARLEAALEKAKTELEAIKDHAFAELGADKAAIFEAHLLV
LNDPELVNPVKDKVNSEKVNAEFAMDEVASMFISMFENMDNEYMKERAADIRDVTKRVLAHLLGINFSNPGTNSEEVIII
AEDLTPSDTAQLNRKYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVVMEKIQNGDIVIIDGLDGEVIVNPSEETLRSF
EEKKAKFEEQKAEWAKLKDQATVTSDGHHVELVANIGTLNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKA
VLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLDEQDVFRTQLRALLRASVYGNLKIMFPMIATLDE
FRQAKAILLEEKAKLVEVGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYN
LSILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMETLAEKALMMSTA
EEVVELVKSI
>Mature_569_residues
TLNIQGIAASSGIAIAKAFRLENPEFNIEQKSITNEAAEIARLEAALEKAKTELEAIKDHAFAELGADKAAIFEAHLLVL
NDPELVNPVKDKVNSEKVNAEFAMDEVASMFISMFENMDNEYMKERAADIRDVTKRVLAHLLGINFSNPGTNSEEVIIIA
EDLTPSDTAQLNRKYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVVMEKIQNGDIVIIDGLDGEVIVNPSEETLRSFE
EKKAKFEEQKAEWAKLKDQATVTSDGHHVELVANIGTLNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKAV
LEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLDEQDVFRTQLRALLRASVYGNLKIMFPMIATLDEF
RQAKAILLEEKAKLVEVGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNL
SILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMETLAEKALMMSTAE
EVVELVKSI

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=50.9734513274336, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI48994992, Length=529, Percent_Identity=38.9413988657845, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788726, Length=568, Percent_Identity=35.0352112676056, Blast_Score=341, Evalue=7e-95,
Organism=Escherichia coli, GI1789193, Length=569, Percent_Identity=33.3919156414763, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1787994, Length=432, Percent_Identity=28.4722222222222, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI226510935, Length=205, Percent_Identity=29.7560975609756, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62843; Mature: 62712

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNIQGIAASSGIAIAKAFRLENPEFNIEQKSITNEAAEIARLEAALEKAKTELEAIKD
CEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFAELGADKAAIFEAHLLVLNDPELVNPVKDKVNSEKVNAEFAMDEVASMFISMFENMD
HHHHHHCCCHHEEEEEEEEEECCCHHCCHHHHCCCCHHCCHHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGINFSNPGTNSEEVIIIAEDLTPSDTAQLNRKYAKGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
TTDIGGRTSHSAIMARSMEIPAVVGTKVVMEKIQNGDIVIIDGLDGEVIVNPSEETLRSF
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHH
EEKKAKFEEQKAEWAKLKDQATVTSDGHHVELVANIGTLNDVQGIIDNGGEGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEEE
LYMGRDNLPTEEEQFEAYKAVLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYR
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHH
AIRLCLDEQDVFRTQLRALLRASVYGNLKIMFPMIATLDEFRQAKAILLEEKAKLVEVGT
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
TVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYN
CCCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
LSILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLS
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEHHHHHHCCCCHHHCCHHHHCCHHHHHH
KLSKAEMETLAEKALMMSTAEEVVELVKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLNIQGIAASSGIAIAKAFRLENPEFNIEQKSITNEAAEIARLEAALEKAKTELEAIKD
EEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFAELGADKAAIFEAHLLVLNDPELVNPVKDKVNSEKVNAEFAMDEVASMFISMFENMD
HHHHHHCCCHHEEEEEEEEEECCCHHCCHHHHCCCCHHCCHHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGINFSNPGTNSEEVIIIAEDLTPSDTAQLNRKYAKGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
TTDIGGRTSHSAIMARSMEIPAVVGTKVVMEKIQNGDIVIIDGLDGEVIVNPSEETLRSF
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHH
EEKKAKFEEQKAEWAKLKDQATVTSDGHHVELVANIGTLNDVQGIIDNGGEGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEEE
LYMGRDNLPTEEEQFEAYKAVLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYR
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHH
AIRLCLDEQDVFRTQLRALLRASVYGNLKIMFPMIATLDEFRQAKAILLEEKAKLVEVGT
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
TVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYN
CCCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
LSILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLS
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEHHHHHHCCCCHHHCCHHHHCCHHHHHH
KLSKAEMETLAEKALMMSTAEEVVELVKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA