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The map label for this gene is glcA [H]

Identifier: 49183602

GI number: 49183602

Start: 624357

End: 625976

Strand: Reverse

Name: glcA [H]

Synonym: BAS0577

Alternate gene names: 49183602

Gene position: 625976-624357 (Counterclockwise)

Preceding gene: 49183605

Following gene: 49183598

Centisome position: 11.97

GC content: 35.8

Gene sequence:

>1620_bases
ATGAGTACTTGGACACAAATTTATGACCCATTAAACAATATTTGGTTATCTGCTCTAATCGCAGCACTTCCAATTTTTTG
CTTCATTATTTGTTTAGTGGGATTCAAAATGAAAGGTTACATGGCTGGTCTTTACAGTGTAATTGTTGCTATTATTCTTG
CTATATTTGTTTATAAAATGCCTACAACTGCAGCAGTAGCATCTGCCGGATTTGGTGTTTTATCTGGATTTTATCCAATT
TGTACTATCGTTATCGCAGCTATCTTCCTTTACAAATTAACTGTTAAAACAGAACAATTCAATGTCATTCGTGATAGCAT
TTCAAGTATTACAAATGACCAACGTTTACAAGTATTATTAATCGCTTATTCTTTCGGCGCTTTCTTAGAAGGCGCAGCTG
GTTTCGGTGTTCCGGTTGCTATTACAGCTGCATTACTTGTAGGTATGGGCTTTAATCCATTAAAAGCAGCGGGTATTTGC
TTAGTAGCTAATATCGCTGGTGGCGCTATGGGGGCTATGGGTATTCCAGTTACAGTACCTGCACAATTAACTGGAATAGA
TGCATTAACTGTCGGCCGTCAAGCAGTTTACATTTTGCCATTCATTAGCATTGTTTTACCGTTCTTACTTGTTTCAATGG
TAGATGGATTTAAAGGCATTAAAGAAACTTGGCAAGGTATTCTTGTAAGTGGTGTTTCTTTCGCAATTACTCAGTTCGTT
GTAACTTACTTCTTAGGCGCTGAACTTACTAATATTTTCGCAGCAGTCGTAAGTATGGTCGCTCTTGCATTATTCTTACG
TGTTTGGCAACCAAAATCTTCTGCTAAAGAAGAAAAGCAAGAAGAAAAAGAATCTCATACATTCAATCAAATTTTATATG
CTTGGTCTCCATTCGTATTCTTAACTGCATTTGTAACAATCTTTAATTTAAAACCAATTAAAGCTTTATTTGCTCCGGAT
GGTCCATTAGCAAACTTAGTATTTAACATTCAATTCCCTGGTCTTCATAATCAAGTTATAAAAACTGCACCAATCACAAA
AGCTGATACACCTTTTGCAGCGATTTTCAAACTAGATGTATTCTCTTCTACAACTACTGCGATTGTATTAGCAATTATTG
TTTCACTTATCGTCTACCGTGTAAACGGCAAAATGATTAAAGAATTAATGATTGAAACAATAAAAGAATTAAAGGTTCCA
GTATATACAATTTGTAGCGTTATCGCTTTAGCTTACGTAGAGAACTACTCTGGTATGTCATCTACACTTGGACTTGCATT
CTCTTCTACTGGTAATGCATTCCCTATTCTTTCTCCAATCTTAGGATGGATTGGCGTATTCATTACAGGTTCAGTAGTAT
CAAGTGGTTCACTATTCGCACCACTTCAAGCAGTAACTGCCGATCAATTAAACATCGTCCCATCTACTCTCGTTTCATTA
AACGTAGTTGGTGGCACAATAGCAAAAATGGTTTCACCACAATCCGTAGCAGTTGCTTGTGCGGCAGTTGGACTAGTTGG
TAAAGAATCTGCTCTATTTAAAACAGTGATGAAATATAGTTTAATCTTCTTAGCTATTATTAGCTTACTTCAATTAAACG
CTGTATTTAATATCTTTTAA

Upstream 100 bases:

>100_bases
AGTGTTGAAGGAGAATATACTAAGCATGTAAGTTGATTGTGAATAGTTTCACAAATAATGTGAATTTCTTCACATAAAAT
AGATAGGAGTTGTCATTATT

Downstream 100 bases:

>100_bases
AAATAAAACACCGATCCAAATGGATCGGTGTTTTTCACATTACATCTTCTTATCCTTTAATAAAGAAGCCCCAGCAATTT
CAGGATGCGTCATTTCGAAT

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSTWTQIYDPLNNIWLSALIAALPIFCFIICLVGFKMKGYMAGLYSVIVAIILAIFVYKMPTTAAVASAGFGVLSGFYPI
CTIVIAAIFLYKLTVKTEQFNVIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGFGVPVAITAALLVGMGFNPLKAAGIC
LVANIAGGAMGAMGIPVTVPAQLTGIDALTVGRQAVYILPFISIVLPFLLVSMVDGFKGIKETWQGILVSGVSFAITQFV
VTYFLGAELTNIFAAVVSMVALALFLRVWQPKSSAKEEKQEEKESHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPD
GPLANLVFNIQFPGLHNQVIKTAPITKADTPFAAIFKLDVFSSTTTAIVLAIIVSLIVYRVNGKMIKELMIETIKELKVP
VYTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPILSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVSL
NVVGGTIAKMVSPQSVAVACAAVGLVGKESALFKTVMKYSLIFLAIISLLQLNAVFNIF

Sequences:

>Translated_539_residues
MSTWTQIYDPLNNIWLSALIAALPIFCFIICLVGFKMKGYMAGLYSVIVAIILAIFVYKMPTTAAVASAGFGVLSGFYPI
CTIVIAAIFLYKLTVKTEQFNVIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGFGVPVAITAALLVGMGFNPLKAAGIC
LVANIAGGAMGAMGIPVTVPAQLTGIDALTVGRQAVYILPFISIVLPFLLVSMVDGFKGIKETWQGILVSGVSFAITQFV
VTYFLGAELTNIFAAVVSMVALALFLRVWQPKSSAKEEKQEEKESHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPD
GPLANLVFNIQFPGLHNQVIKTAPITKADTPFAAIFKLDVFSSTTTAIVLAIIVSLIVYRVNGKMIKELMIETIKELKVP
VYTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPILSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVSL
NVVGGTIAKMVSPQSVAVACAAVGLVGKESALFKTVMKYSLIFLAIISLLQLNAVFNIF
>Mature_538_residues
STWTQIYDPLNNIWLSALIAALPIFCFIICLVGFKMKGYMAGLYSVIVAIILAIFVYKMPTTAAVASAGFGVLSGFYPIC
TIVIAAIFLYKLTVKTEQFNVIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGFGVPVAITAALLVGMGFNPLKAAGICL
VANIAGGAMGAMGIPVTVPAQLTGIDALTVGRQAVYILPFISIVLPFLLVSMVDGFKGIKETWQGILVSGVSFAITQFVV
TYFLGAELTNIFAAVVSMVALALFLRVWQPKSSAKEEKQEEKESHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPDG
PLANLVFNIQFPGLHNQVIKTAPITKADTPFAAIFKLDVFSSTTTAIVLAIIVSLIVYRVNGKMIKELMIETIKELKVPV
YTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPILSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVSLN
VVGGTIAKMVSPQSVAVACAAVGLVGKESALFKTVMKYSLIFLAIISLLQLNAVFNIF

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=550, Percent_Identity=50.9090909090909, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1790031, Length=538, Percent_Identity=49.0706319702602, Blast_Score=501, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 57560; Mature: 57429

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWTQIYDPLNNIWLSALIAALPIFCFIICLVGFKMKGYMAGLYSVIVAIILAIFVYKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTTAAVASAGFGVLSGFYPICTIVIAAIFLYKLTVKTEQFNVIRDSISSITNDQRLQVLL
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHEEEE
IAYSFGAFLEGAAGFGVPVAITAALLVGMGFNPLKAAGICLVANIAGGAMGAMGIPVTVP
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCCCCCCCCEECC
AQLTGIDALTVGRQAVYILPFISIVLPFLLVSMVDGFKGIKETWQGILVSGVSFAITQFV
CHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTYFLGAELTNIFAAVVSMVALALFLRVWQPKSSAKEEKQEEKESHTFNQILYAWSPFVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTAFVTIFNLKPIKALFAPDGPLANLVFNIQFPGLHNQVIKTAPITKADTPFAAIFKLDV
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHEEEHH
FSSTTTAIVLAIIVSLIVYRVNGKMIKELMIETIKELKVPVYTICSVIALAYVENYSGMS
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
STLGLAFSSTGNAFPILSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVSL
HHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
NVVGGTIAKMVSPQSVAVACAAVGLVGKESALFKTVMKYSLIFLAIISLLQLNAVFNIF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STWTQIYDPLNNIWLSALIAALPIFCFIICLVGFKMKGYMAGLYSVIVAIILAIFVYKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTTAAVASAGFGVLSGFYPICTIVIAAIFLYKLTVKTEQFNVIRDSISSITNDQRLQVLL
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHEEEE
IAYSFGAFLEGAAGFGVPVAITAALLVGMGFNPLKAAGICLVANIAGGAMGAMGIPVTVP
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCCCCCCCCEECC
AQLTGIDALTVGRQAVYILPFISIVLPFLLVSMVDGFKGIKETWQGILVSGVSFAITQFV
CHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTYFLGAELTNIFAAVVSMVALALFLRVWQPKSSAKEEKQEEKESHTFNQILYAWSPFVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTAFVTIFNLKPIKALFAPDGPLANLVFNIQFPGLHNQVIKTAPITKADTPFAAIFKLDV
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHEEEHH
FSSTTTAIVLAIIVSLIVYRVNGKMIKELMIETIKELKVPVYTICSVIALAYVENYSGMS
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
STLGLAFSSTGNAFPILSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVSL
HHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
NVVGGTIAKMVSPQSVAVACAAVGLVGKESALFKTVMKYSLIFLAIISLLQLNAVFNIF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]