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The map label for this gene is nupC [H]

Identifier: 49183598

GI number: 49183598

Start: 619951

End: 621129

Strand: Reverse

Name: nupC [H]

Synonym: BAS0573

Alternate gene names: 49183598

Gene position: 621129-619951 (Counterclockwise)

Preceding gene: 49183602

Following gene: 49183597

Centisome position: 11.88

GC content: 35.79

Gene sequence:

>1179_bases
ATGAAATTTATTACTTTTTTCTTAGGACTTATCGTCGTCTTCTTCCTTGCTTATATCGCTAGTAACAATAAGAAGCATAT
TAAATTTAAACCTATTTTCATCATGCTTCTTATACAGTTAATTTTAACCTATTTATTATTGAATACAGAAATCGGTCTCA
TACTTATTCGGGTCATCTCCAGTTTGTTTACAAAGCTACTCGAGTATGCTGCTGATGGTATAAACTTCGTATTTGGCGGC
CTTGCCAATAAAGGTGAAATGCCCTTTTTCCTTACTGTCTTATTACCAATTGTCTTCATTTCCGTCTTAATTGGTATACT
ACAACATTTCAAAATACTACCATTTTTCATTCATTGGATCGGTTACTTCCTGAGCAAAATAAATGGTCTTGGGAAATTAG
AATCTTATAATGCTATCGCCTCTGCCATTGTCGGCCAATCAGAAGTTTTTATTACAGTCAAAAAACAATTAGCTCAAATT
CCAAAACACCGTCTTTATACACTTTGTGCATCTGCCATGTCAACCGTATCTATGTCTATCGTAGGTGCCTATATGACAAT
GATTGAACCTAAATATGTAGTAACCGCACTCGTTCTCAATTTATTTAGCGGTTTTATTATCGTACTTATCATTAACCCTT
ACGACGTTAAAGATGACGAAGATATTTTAGAGATTAAAGGCGAAAAGCAAAGCTTTTTTGAAATGCTTGGAGAATACATT
TTAGATGGCTTTCGCGTAGCTATCGTTGTCGGGGCTATGCTTATCGGATTCGTCGCATTAATTAGCTGCATTAATGATCT
ATTCCTCATTATATTCGGCATTACTTTCCAACAATTAATCGGCTACGTCTTTGCGCCTATTGCATTCCTTATCGGTGTAC
CAAGTTCTGAAATTGTCGCGGCTGGTAGCATTATGGCAACGAAGCTTGTAACGAATGAATTTGTAGCAATGATGGACCTT
AGTAAAATCTCTAATAGCCTTTCTCCCCGTACAGTTGGTATTATTTCTGTTTTCCTCGTTTCTTTTGCCAACTTTTCTTC
TATCGGCATTATTTCAGGTGCGGTAAAAGGATTAAACGAAGAACAAGGAAACGTTGTTGCAAGGTTTGGCCTTAAATTAC
TATATGGAGCTACTCTCGTTAGTATTTTATCTGCAATTATCGTAAGCATTATGTTGTAA

Upstream 100 bases:

>100_bases
TATATTCATGACTTCTTTATTCCTTTCTTTACCAAAGATGCATATTCTCCCTTGAAATGAAAAACATATATAGCAGAATA
TCTCTTTTAGGGGGCTTACT

Downstream 100 bases:

>100_bases
TTAGGTAAAATTTTATCATTTTCTTATCCCTATCTTTTGTATACAATAACGAATAGGTGTCTTATAAAATGAAACTTTAA
TCAGATTCGGCTATCCCTAC

Product: NupC family nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKFITFFLGLIVVFFLAYIASNNKKHIKFKPIFIMLLIQLILTYLLLNTEIGLILIRVISSLFTKLLEYAADGINFVFGG
LANKGEMPFFLTVLLPIVFISVLIGILQHFKILPFFIHWIGYFLSKINGLGKLESYNAIASAIVGQSEVFITVKKQLAQI
PKHRLYTLCASAMSTVSMSIVGAYMTMIEPKYVVTALVLNLFSGFIIVLIINPYDVKDDEDILEIKGEKQSFFEMLGEYI
LDGFRVAIVVGAMLIGFVALISCINDLFLIIFGITFQQLIGYVFAPIAFLIGVPSSEIVAAGSIMATKLVTNEFVAMMDL
SKISNSLSPRTVGIISVFLVSFANFSSIGIISGAVKGLNEEQGNVVARFGLKLLYGATLVSILSAIIVSIML

Sequences:

>Translated_392_residues
MKFITFFLGLIVVFFLAYIASNNKKHIKFKPIFIMLLIQLILTYLLLNTEIGLILIRVISSLFTKLLEYAADGINFVFGG
LANKGEMPFFLTVLLPIVFISVLIGILQHFKILPFFIHWIGYFLSKINGLGKLESYNAIASAIVGQSEVFITVKKQLAQI
PKHRLYTLCASAMSTVSMSIVGAYMTMIEPKYVVTALVLNLFSGFIIVLIINPYDVKDDEDILEIKGEKQSFFEMLGEYI
LDGFRVAIVVGAMLIGFVALISCINDLFLIIFGITFQQLIGYVFAPIAFLIGVPSSEIVAAGSIMATKLVTNEFVAMMDL
SKISNSLSPRTVGIISVFLVSFANFSSIGIISGAVKGLNEEQGNVVARFGLKLLYGATLVSILSAIIVSIML
>Mature_392_residues
MKFITFFLGLIVVFFLAYIASNNKKHIKFKPIFIMLLIQLILTYLLLNTEIGLILIRVISSLFTKLLEYAADGINFVFGG
LANKGEMPFFLTVLLPIVFISVLIGILQHFKILPFFIHWIGYFLSKINGLGKLESYNAIASAIVGQSEVFITVKKQLAQI
PKHRLYTLCASAMSTVSMSIVGAYMTMIEPKYVVTALVLNLFSGFIIVLIINPYDVKDDEDILEIKGEKQSFFEMLGEYI
LDGFRVAIVVGAMLIGFVALISCINDLFLIIFGITFQQLIGYVFAPIAFLIGVPSSEIVAAGSIMATKLVTNEFVAMMDL
SKISNSLSPRTVGIISVFLVSFANFSSIGIISGAVKGLNEEQGNVVARFGLKLLYGATLVSILSAIIVSIML

Specific function: Transports Nucleosides With A High Affinity Except Guanosine And Deoxyguanosine. Driven By A Proton Motive Force. [C]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=411, Percent_Identity=24.8175182481752, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI11545853, Length=413, Percent_Identity=25.181598062954, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI227116277, Length=412, Percent_Identity=22.3300970873786, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788737, Length=398, Percent_Identity=59.7989949748744, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1788485, Length=408, Percent_Identity=26.7156862745098, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788488, Length=408, Percent_Identity=27.6960784313726, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17560276, Length=392, Percent_Identity=24.7448979591837, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71991794, Length=405, Percent_Identity=23.7037037037037, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45552519, Length=392, Percent_Identity=23.2142857142857, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI45552517, Length=392, Percent_Identity=23.2142857142857, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19921868, Length=392, Percent_Identity=23.2142857142857, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281360430, Length=353, Percent_Identity=23.5127478753541, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43088; Mature: 43088

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFITFFLGLIVVFFLAYIASNNKKHIKFKPIFIMLLIQLILTYLLLNTEIGLILIRVIS
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFTKLLEYAADGINFVFGGLANKGEMPFFLTVLLPIVFISVLIGILQHFKILPFFIHWI
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYFLSKINGLGKLESYNAIASAIVGQSEVFITVKKQLAQIPKHRLYTLCASAMSTVSMSI
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
VGAYMTMIEPKYVVTALVLNLFSGFIIVLIINPYDVKDDEDILEIKGEKQSFFEMLGEYI
HHHHHHHHCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LDGFRVAIVVGAMLIGFVALISCINDLFLIIFGITFQQLIGYVFAPIAFLIGVPSSEIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGSIMATKLVTNEFVAMMDLSKISNSLSPRTVGIISVFLVSFANFSSIGIISGAVKGLNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EQGNVVARFGLKLLYGATLVSILSAIIVSIML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFITFFLGLIVVFFLAYIASNNKKHIKFKPIFIMLLIQLILTYLLLNTEIGLILIRVIS
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFTKLLEYAADGINFVFGGLANKGEMPFFLTVLLPIVFISVLIGILQHFKILPFFIHWI
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYFLSKINGLGKLESYNAIASAIVGQSEVFITVKKQLAQIPKHRLYTLCASAMSTVSMSI
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
VGAYMTMIEPKYVVTALVLNLFSGFIIVLIINPYDVKDDEDILEIKGEKQSFFEMLGEYI
HHHHHHHHCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LDGFRVAIVVGAMLIGFVALISCINDLFLIIFGITFQQLIGYVFAPIAFLIGVPSSEIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGSIMATKLVTNEFVAMMDLSKISNSLSPRTVGIISVFLVSFANFSSIGIISGAVKGLNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EQGNVVARFGLKLLYGATLVSILSAIIVSIML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550462; 8867804; 9384377 [H]