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The map label for this gene is thiE [H]

Identifier: 49183391

GI number: 49183391

Start: 394932

End: 395591

Strand: Direct

Name: thiE [H]

Synonym: BAS0363

Alternate gene names: 49183391

Gene position: 394932-395591 (Clockwise)

Preceding gene: 49183390

Following gene: 49183393

Centisome position: 7.55

GC content: 38.79

Gene sequence:

>660_bases
ATGTCACGTATTTCAAAGGCAGAAATGTCTAAGTTATTATCAGTATATTTTATTATGGGAAGTAACAATTGTACGAAGGA
TCCACTGCAAGTATTGAGAGAGGCACTTGAAGGTTTTATAACAATTTTTCAATTTCGTGAAAAGGGAGAAGGTGCTTTAA
CGGGAGAAGAGCGTATTTGTTTCGCAAAAGAATTACAAGCAATTTGTAAGGAATACGGTGTACCATTCATCGTAAATGAT
GATGTGGAACTAGCCCTCGAGTTAGATGCAGATGGCGTGCATGTTGGACAAGATGATGAAGGAATTACTTCTGTTCGTGA
AAAAATGGGTGATAAAATTGTTGGTGTATCTACCCATACAATTGAAGAAGCACGCTGGGTAATTGAAAACGGAGCTGATT
ATTTAGGAGTTGGCCCAATTTTTCCGACGAGTACGAAAAAGGATACGAAAGCGGTGCAAGGAACGAAAGGTTTAGCTCAT
TTTAGAGAGCAAGGAATTACAATACCGATTGTTGGGATTGGTGGCATTTCAATTGAAAATACAGCTTCGGTTATAGAAGC
AGGTGCGGATGGTGTTTCCGTTATTTCTGCGATTAGTTTAGCGGAATCTGCCTATGAAAGTACGAAGAAATTAGTAGAGG
AAGTAAGTAGGAGTTTGTAG

Upstream 100 bases:

>100_bases
AGTTTCCAAATTGAATTTTTAAATCAGTTAGCCAATACGACTTCGGGTGATATAGAAAAGTATGGGAAGATTGAGGTTAT
ATAGTAAGGAGGGAAAATAA

Downstream 100 bases:

>100_bases
GAAAAGGGAACTTCACAAAAATAGTAAAGAACAGAATGGACAGATAAAAATATTTAAACAAACGTTTGATTAAAAAAGCA
CAAACCCTTTAAATTTGTAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSRISKAEMSKLLSVYFIMGSNNCTKDPLQVLREALEGFITIFQFREKGEGALTGEERICFAKELQAICKEYGVPFIVND
DVELALELDADGVHVGQDDEGITSVREKMGDKIVGVSTHTIEEARWVIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAH
FREQGITIPIVGIGGISIENTASVIEAGADGVSVISAISLAESAYESTKKLVEEVSRSL

Sequences:

>Translated_219_residues
MSRISKAEMSKLLSVYFIMGSNNCTKDPLQVLREALEGFITIFQFREKGEGALTGEERICFAKELQAICKEYGVPFIVND
DVELALELDADGVHVGQDDEGITSVREKMGDKIVGVSTHTIEEARWVIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAH
FREQGITIPIVGIGGISIENTASVIEAGADGVSVISAISLAESAYESTKKLVEEVSRSL
>Mature_218_residues
SRISKAEMSKLLSVYFIMGSNNCTKDPLQVLREALEGFITIFQFREKGEGALTGEERICFAKELQAICKEYGVPFIVNDD
VELALELDADGVHVGQDDEGITSVREKMGDKIVGVSTHTIEEARWVIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAHF
REQGITIPIVGIGGISIENTASVIEAGADGVSVISAISLAESAYESTKKLVEEVSRSL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=188, Percent_Identity=31.3829787234043, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=197, Percent_Identity=38.0710659898477, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23600; Mature: 23469

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISKAEMSKLLSVYFIMGSNNCTKDPLQVLREALEGFITIFQFREKGEGALTGEERIC
CCCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FAKELQAICKEYGVPFIVNDDVELALELDADGVHVGQDDEGITSVREKMGDKIVGVSTHT
HHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCEEECCCHHH
IEEARWVIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAHFREQGITIPIVGIGGISIEN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCH
TASVIEAGADGVSVISAISLAESAYESTKKLVEEVSRSL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRISKAEMSKLLSVYFIMGSNNCTKDPLQVLREALEGFITIFQFREKGEGALTGEERIC
CCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FAKELQAICKEYGVPFIVNDDVELALELDADGVHVGQDDEGITSVREKMGDKIVGVSTHT
HHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCEEECCCHHH
IEEARWVIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAHFREQGITIPIVGIGGISIEN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCH
TASVIEAGADGVSVISAISLAESAYESTKKLVEEVSRSL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA