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The map label for this gene is thiM [H]

Identifier: 49183390

GI number: 49183390

Start: 394109

End: 394915

Strand: Direct

Name: thiM [H]

Synonym: BAS0362

Alternate gene names: 49183390

Gene position: 394109-394915 (Clockwise)

Preceding gene: 49183388

Following gene: 49183391

Centisome position: 7.54

GC content: 40.27

Gene sequence:

>807_bases
ATGAATACGAAAGAAATTAGCAAAGTAGTGGATTTGGTGAGAGAATCTAATCCGCTCGTTCATAATATTACAAATGTTGT
TGTAACAAATTTTACAGCTAACGGTTTATTAGCATTAGGGGCATCGCCTGTAATGGCGTATGCAAAAGAAGAAGTAGCAG
AAATGGCTAGCATTGCTGGAGCGTTAGTATTAAATATGGGGACACTTCGTCCTGACGAGGTAGAAGCGATGTTACTTGCT
GGTAAGTCGGCTAATAGGAATGATGTACCGGTACTGTTTGATCCGGTTGGCGCAGGGGCAACTTCGTATCGAACAGAAGT
TGCGAGACATATTCCGGCTGAAATCGAATTAGCAATTATTCGCGGAAATGCAGCTGAAATAGCGAACGTTATTAATGAAA
AATGGGAAATTAAAGGGGTAGACGCTGGTGCTGGAAATGGTAATGTCGTAAGTATTGCAAAGCAGGCAGCAGATGAACTA
AATACAGTTGCGGTGATTACTGGAAAAGAAGATGTTGTTACAGATGGAGAGCGAACTATTGTTATTCGAAATGGTCACTC
TATTTTAACGAAAATTACGGGAACTGGTTGTTTACTAACTTCTGTAATAGGAGCATTTGTAGCGGTAGAAAAGGATTATG
TAAAGGCTGCAGTAGCGGCATTAACGTTTTATGGTGTAGCCGCGGAACTAGCAGCTGCTAAGACAGTAGAAAAAGGACCT
GGTAGTTTCCAAATTGAATTTTTAAATCAGTTAGCCAATACGACTTCGGGTGATATAGAAAAGTATGGGAAGATTGAGGT
TATATAG

Upstream 100 bases:

>100_bases
CAGCGTAGGGAGAGTGTAAAAGCACATGTTCAGGCACTTTGCGTACGCGCAAAGTGCCTTTTTGTGTATCTCCCATCACT
ATAGTTAGGAGATGAGAAGG

Downstream 100 bases:

>100_bases
TAAGGAGGGAAAATAAATGTCACGTATTTCAAAGGCAGAAATGTCTAAGTTATTATCAGTATATTTTATTATGGGAAGTA
ACAATTGTACGAAGGATCCA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNTKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLLA
GKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEVI

Sequences:

>Translated_268_residues
MNTKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLLA
GKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEVI
>Mature_268_residues
MNTKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLLA
GKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEVI

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=259, Percent_Identity=38.6100386100386, Blast_Score=161, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6325042, Length=279, Percent_Identity=34.0501792114695, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28059; Mature: 28059

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPDEVEAMLLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAII
HHHHHCCCCCCHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
RGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADELNTVAVITGKEDVVTDGERTI
ECCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCCEEE
VIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVEKGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSFQIEFLNQLANTTSGDIEKYGKIEVI
CCEEHHHHHHHHCCCCCCHHHCCCEEEC
>Mature Secondary Structure
MNTKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPDEVEAMLLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAII
HHHHHCCCCCCHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
RGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADELNTVAVITGKEDVVTDGERTI
ECCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCCEEE
VIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVEKGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSFQIEFLNQLANTTSGDIEKYGKIEVI
CCEEHHHHHHHHCCCCCCHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA