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The map label for this gene is yisK [H]

Identifier: 49183260

GI number: 49183260

Start: 228024

End: 228980

Strand: Direct

Name: yisK [H]

Synonym: BAS0227

Alternate gene names: 49183260

Gene position: 228024-228980 (Clockwise)

Preceding gene: 49183259

Following gene: 49183261

Centisome position: 4.36

GC content: 39.92

Gene sequence:

>957_bases
ATGAAATTTGTTACATTTCGTCTTCCTTCAAAAGAAATGCGAGCTGGATGGCTTGAAGGTGACAAAGTAATCGATATGAA
TCTTGCTAGTGATGGGAAATTACCTTCTTCTATGTTCGCCTTTTTAGAGAAAGCGGATGAGTATGTAGAAGTATTGCGTA
ATATTAAGAACCCGGAAAAGGGCATATATTCTTTAGAGGAAGTGCAATTAGCAGCAGCGATTCCTAATCCGAGTAGTATT
CGGGATTTTTACGCGTTTGAGCAGCATGTAAAAACGGCACGTGGCCGAAGAGGGCTAGATGTTGTACCAGAATGGTATGA
TATCCCGGTTTTTTATTTTACGAACCATCGTGCTGTAATTGGTCCGAATGATGTTGTAATTGGTCCGAAAAAATCTGAAA
AAATTGATTATGAGTTAGAGATTGCTTGCGTAATCGGGAAAGAAGGAAGAAATATTTCTCGTGAGCAAGCAGAGGAATAC
ATTTTTGGTTATTGTATTATGAATGATTGGAGTGCAAGGGACTTACAAGCAACAGAAATGAAGGTAGGACTTGGTCCAGC
GAAAGGAAAAGATTTTGCAACTTCATTAGGAGCGCACCTTGTTACGAAAGAGGAATTAGATGTTTACCGCAACGGTGACC
GTTATGAGTTAGAGATGACTGCTCATGTGAATGGAAAGCTATTATCAAAAGGGAACTTCCAAGATATTTACTATACATTT
GCTGAAATGATTGAACGTGCTTCGGAAGATGTTACGTTATACCCAGGTGATGTGATTGGTTCTGGGACAGTAGGAACAGG
TTGTATTTTAGAACTGGGTACAGAAGAGTGGCTGCAAGATGGTGATGTGGTAGAGCTTACGATTACTGGTTTAGGTACAT
TACGTAATACGGTCAAAAAAGAAAAGGAAGCAGGTGATGAGCATGTTTTATCGTCACATGGGGGAGCTACCTCATAA

Upstream 100 bases:

>100_bases
TGAAAGAGAACAAGAGCGTCGCGGGAATTTATAAAATTTATATCCACCAGCAATAATTCTTTTGCTGGTGGAAATTTCAT
TTTCATAGGGAGGAATCATG

Downstream 100 bases:

>100_bases
ACGACATGTACAATTCCGTAAAAAAGATGGATCGCTTTATCGTGAACAGGTAATGGGAACAAAAGGTTTTTCTGGTACGC
AATCTATTTTGTATCATCAT

Product: fumarylacetoacetate hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKFVTFRLPSKEMRAGWLEGDKVIDMNLASDGKLPSSMFAFLEKADEYVEVLRNIKNPEKGIYSLEEVQLAAAIPNPSSI
RDFYAFEQHVKTARGRRGLDVVPEWYDIPVFYFTNHRAVIGPNDVVIGPKKSEKIDYELEIACVIGKEGRNISREQAEEY
IFGYCIMNDWSARDLQATEMKVGLGPAKGKDFATSLGAHLVTKEELDVYRNGDRYELEMTAHVNGKLLSKGNFQDIYYTF
AEMIERASEDVTLYPGDVIGSGTVGTGCILELGTEEWLQDGDVVELTITGLGTLRNTVKKEKEAGDEHVLSSHGGATS

Sequences:

>Translated_318_residues
MKFVTFRLPSKEMRAGWLEGDKVIDMNLASDGKLPSSMFAFLEKADEYVEVLRNIKNPEKGIYSLEEVQLAAAIPNPSSI
RDFYAFEQHVKTARGRRGLDVVPEWYDIPVFYFTNHRAVIGPNDVVIGPKKSEKIDYELEIACVIGKEGRNISREQAEEY
IFGYCIMNDWSARDLQATEMKVGLGPAKGKDFATSLGAHLVTKEELDVYRNGDRYELEMTAHVNGKLLSKGNFQDIYYTF
AEMIERASEDVTLYPGDVIGSGTVGTGCILELGTEEWLQDGDVVELTITGLGTLRNTVKKEKEAGDEHVLSSHGGATS
>Mature_318_residues
MKFVTFRLPSKEMRAGWLEGDKVIDMNLASDGKLPSSMFAFLEKADEYVEVLRNIKNPEKGIYSLEEVQLAAAIPNPSSI
RDFYAFEQHVKTARGRRGLDVVPEWYDIPVFYFTNHRAVIGPNDVVIGPKKSEKIDYELEIACVIGKEGRNISREQAEEY
IFGYCIMNDWSARDLQATEMKVGLGPAKGKDFATSLGAHLVTKEELDVYRNGDRYELEMTAHVNGKLLSKGNFQDIYYTF
AEMIERASEDVTLYPGDVIGSGTVGTGCILELGTEEWLQDGDVVELTITGLGTLRNTVKKEKEAGDEHVLSSHGGATS

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=305, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI40786394, Length=305, Percent_Identity=30.4918032786885, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4557587, Length=289, Percent_Identity=28.0276816608997, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI13654274, Length=194, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI215422413, Length=156, Percent_Identity=28.8461538461538, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI66348062, Length=156, Percent_Identity=28.8461538461538, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568863, Length=270, Percent_Identity=28.8888888888889, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28572127, Length=190, Percent_Identity=37.3684210526316, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24657182, Length=274, Percent_Identity=28.4671532846715, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24663695, Length=305, Percent_Identity=24.5901639344262, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571789, Length=197, Percent_Identity=25.3807106598985, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 35452; Mature: 35452

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVTFRLPSKEMRAGWLEGDKVIDMNLASDGKLPSSMFAFLEKADEYVEVLRNIKNPEK
CCEEEEECCCHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
GIYSLEEVQLAAAIPNPSSIRDFYAFEQHVKTARGRRGLDVVPEWYDIPVFYFTNHRAVI
HCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEE
GPNDVVIGPKKSEKIDYELEIACVIGKEGRNISREQAEEYIFGYCIMNDWSARDLQATEM
CCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHEEEEEEEECCCCCCCCEEEEE
KVGLGPAKGKDFATSLGAHLVTKEELDVYRNGDRYELEMTAHVNGKLLSKGNFQDIYYTF
EECCCCCCCCHHHHHHHHHHEEHHHHHHHCCCCEEEEEEEEECCCEEECCCCCHHHHHHH
AEMIERASEDVTLYPGDVIGSGTVGTGCILELGTEEWLQDGDVVELTITGLGTLRNTVKK
HHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCHHHHCCCCEEEEEEECCHHHHHHHHH
EKEAGDEHVLSSHGGATS
HHHCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKFVTFRLPSKEMRAGWLEGDKVIDMNLASDGKLPSSMFAFLEKADEYVEVLRNIKNPEK
CCEEEEECCCHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
GIYSLEEVQLAAAIPNPSSIRDFYAFEQHVKTARGRRGLDVVPEWYDIPVFYFTNHRAVI
HCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEE
GPNDVVIGPKKSEKIDYELEIACVIGKEGRNISREQAEEYIFGYCIMNDWSARDLQATEM
CCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHEEEEEEEECCCCCCCCEEEEE
KVGLGPAKGKDFATSLGAHLVTKEELDVYRNGDRYELEMTAHVNGKLLSKGNFQDIYYTF
EECCCCCCCCHHHHHHHHHHEEHHHHHHHCCCCEEEEEEEEECCCEEECCCCCHHHHHHH
AEMIERASEDVTLYPGDVIGSGTVGTGCILELGTEEWLQDGDVVELTITGLGTLRNTVKK
HHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCHHHHCCCCEEEEEEECCHHHHHHHHH
EKEAGDEHVLSSHGGATS
HHHCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]