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The map label for this gene is pdxT

Identifier: 49183050

GI number: 49183050

Start: 19421

End: 20011

Strand: Direct

Name: pdxT

Synonym: BAS0014

Alternate gene names: 49183050

Gene position: 19421-20011 (Clockwise)

Preceding gene: 49183049

Following gene: 49183051

Centisome position: 0.37

GC content: 40.27

Gene sequence:

>591_bases
ATGGTGAAAATTGGTGTACTAGGTCTTCAAGGTGCAGTTCGTGAACATGTAAAATCAGTTGAAGCAAGTGGTGCAGAAGC
TGTTGTTGTAAAGCGTATAGAACAACTTGAAGAGATTGATGGTCTTATTTTACCAGGCGGTGAAAGTACAACTATGCGCC
GTCTTATTGATAAGTATGATTTCATGGAACCACTTCGTACATTCGCGAAGTCTGGTAAACCAATGTTTGGTACATGTGCA
GGAATGATTCTTCTTGCTAAAACACTTATTGGCTATGACGAAGCACATATTGGTGCGATGGATATTACAGTTGAGCGCAA
TGCGTTTGGACGTCAAAAAGATAGCTTTGAAGCTGCACTTTCTATTAAAGGTGTGGGAGAAGATTTTGTTGGCGTATTTA
TTCGTGCCCCGTATGTTGTAGATGTAGCGGATGATGTTGAGGTACTTTCTACACATGGCGATCGAATGGTAGCGGTAAAG
CAAGGGCCGTTTTTAGCTGCTTCTTTCCATCCAGAATTAACGGATGATCATCGTGTAACAGCATACTTTGTAGAAATGGT
AAAAGAAGCGAAAATGAAAAAAGTTGTATAA

Upstream 100 bases:

>100_bases
AAAAGGATTAGGTAATGCGATGAAGGGTATCGAAATTTCAACGTTATTACCAGAACAACGCATGCAAGAGCGTGGATGGT
AATTGAAGGAGAACTTTAAA

Downstream 100 bases:

>100_bases
GTAACTTGCAACTTGTATAAGATTATAGTAAATTGATGGTAACAATTTTATAAAATAAGCGTGTTGATAGGAAGTAGTAA
CAAATGTCGTTTCTTATAGA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYDFMEPLRTFAKSGKPMFGTCA
GMILLAKTLIGYDEAHIGAMDITVERNAFGRQKDSFEAALSIKGVGEDFVGVFIRAPYVVDVADDVEVLSTHGDRMVAVK
QGPFLAASFHPELTDDHRVTAYFVEMVKEAKMKKVV

Sequences:

>Translated_196_residues
MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYDFMEPLRTFAKSGKPMFGTCA
GMILLAKTLIGYDEAHIGAMDITVERNAFGRQKDSFEAALSIKGVGEDFVGVFIRAPYVVDVADDVEVLSTHGDRMVAVK
QGPFLAASFHPELTDDHRVTAYFVEMVKEAKMKKVV
>Mature_196_residues
MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYDFMEPLRTFAKSGKPMFGTCA
GMILLAKTLIGYDEAHIGAMDITVERNAFGRQKDSFEAALSIKGVGEDFVGVFIRAPYVVDVADDVEVLSTHGDRMVAVK
QGPFLAASFHPELTDDHRVTAYFVEMVKEAKMKKVV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=36.6666666666667, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=36.7924528301887, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323742, Length=206, Percent_Identity=35.4368932038835, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_BACAA (C3P8Q4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864667.1
- ProteinModelPortal:   C3P8Q4
- SMR:   C3P8Q4
- EnsemblBacteria:   EBBACT00000124887
- GeneID:   7849831
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0017
- GeneTree:   EBGT00050000000582
- ProtClustDB:   PRK13525
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21432; Mature: 21432

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 170-170 ACT_SITE 172-172 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYD
CEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMEPLRTFAKSGKPMFGTCAGMILLAKTLIGYDEAHIGAMDITVERNAFGRQKDSFEAAL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHEE
SIKGVGEDFVGVFIRAPYVVDVADDVEVLSTHGDRMVAVKQGPFLAASFHPELTDDHRVT
EECCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCHHH
AYFVEMVKEAKMKKVV
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYD
CEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMEPLRTFAKSGKPMFGTCAGMILLAKTLIGYDEAHIGAMDITVERNAFGRQKDSFEAAL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHEE
SIKGVGEDFVGVFIRAPYVVDVADDVEVLSTHGDRMVAVKQGPFLAASFHPELTDDHRVT
EECCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCHHH
AYFVEMVKEAKMKKVV
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA