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The map label for this gene is gyrB

Identifier: 49183044

GI number: 49183044

Start: 4585

End: 6507

Strand: Direct

Name: gyrB

Synonym: BAS0005

Alternate gene names: 49183044

Gene position: 4585-6507 (Clockwise)

Preceding gene: 49183043

Following gene: 49183045

Centisome position: 0.09

GC content: 36.92

Gene sequence:

>1923_bases
ATGGAACAAAAGCAAATGCAAGAAAATTCATATGATGAAAGTCAAATACAGGTACTTGAAGGACTAGAAGCAGTTCGAAA
ACGCCCTGGTATGTATATTGGATCTACAAGTGGAAAAGGACTTCACCATCTTGTATGGGAAATCGTTGATAATAGTATCG
ATGAAGCACTTGCAGGGTACTGTGACGAAATTAACGTTAGTATCGAAGAAGATAATAGTATTCGTGTAACAGATAATGGA
CGTGGTATTCCAGTTGGTATACAAGAAAAAATGGGACGTCCTGCTGTAGAAGTTATTATGACCGTTCTTCATGCTGGTGG
TAAGTTTGGCGGCGGCGGTTATAAAGTTTCTGGTGGTTTGCATGGTGTTGGGGCATCTGTAGTAAATGCTCTATCAACAG
AACTAGAGGTATTTGTACATCGTGAAGGTAAAATCCATTATCAAAAATACGAAAGAGGTATTCCGGTTGCGGATTTAAAA
GTCATTGGTGATACAGATCAAACGGGAACGATAACTCGATTTAAACCAGATCCAGAAATTTTTCAGGAAACAACAGTATA
CGAATTTGATACACTAGCAACTCGTATGCGTGAATTAGCATTTTTAAATCGTAATATTAAACTGACGATTGAAGATAAAC
GTGAACATAAGCAAAAAAAAGAATTCCATTATGAAGGTGGAATTAAATCATATGTTGAGCATTTAAACCGCTCAAAACAA
CCAATCCATGAAGAGCCTGTATATGTAGAAGGATCAAAAGATGGTATTCAAGTTGAAGTTTCCTTACAGTATAACGAAGG
ATATACAAATAATATTTACTCATTTACGAACAACATTCACACGTATGAAGGTGGAACACATGAAGTAGGGTTTAAAACAG
CTTTAACTCGTGTGATTAACGATTATGGGCGTAAAAATAGTATTCTAAAAGATGCAGACAGTAATTTAACTGGTGAGGAC
GTTCGTGAAGGTTTAACTGCAATTGTATCAATTAAACATCCAAATCCACAATTTGAAGGACAAACGAAGACGAAACTTGG
GAATAGTGAAGCGAGAACGATTACAGAGTCTGTGTTTTCAGAGGCATTTGAAAAGTTCTTACTAGAAAACCCGAACGTTG
CACGAAAAATCGTAGAAAAAGGTACGATGGCAGCGCGTGCACGTGTTGCAGCGAAAAAAGCACGTGAATTGACACGTCGT
AAGAGCGCGTTAGAAGTTTCAAGTTTACCTGGTAAATTAGCAGATTGCTCTTCAAAAGATCCAGCAATTAGTGAAATTTA
CATTGTAGAGGGTGACTCTGCCGGTGGATCAGCAAAGCAAGGGCGTGATCGTCACTTCCAAGCGATTTTACCACTGAAAG
GTAAAATTATTAACGTTGAAAAGGCAAGATTAGATAAAATCTTATCTAACGATGAAGTGCGTACAATTATTACTGCAATT
GGTACGAACATTGGCGGAGATTTTGATATTGAGAAAGCTCGTTATCATAAAGTTATTATTATGACGGATGCCGACGTAGA
TGGTGCGCATATTCGTACCCTATTATTAACGTTCTTCTATCGTTATATGCGTCAAATTATTGAACATGGTTATATCTATA
TTGCACAGCCACCGTTGTTTAAAGTACAACAAGGTAAAAAAATTCAATATGCTTATAATGAGAAAGAGCTTGAAAAGATT
TTAGCTGAATTACCAGCTCAACCTAAACCTGGAATCCAACGTTACAAAGGTTTAGGAGAAATGAATCCAACTCAGCTTTG
GGAAACAACAATGGACCCAGAAGTACGTTCATTACTTCAAGTTTCCCTTCAAGATGCAATTGAAGCAGATGAAACATTTG
AAATTTTAATGGGGGATAAAGTAGAGCCACGTCGTAACTTTATCCAAGAAAATGCAAAATACGTGAAAAACCTTGATATT
TAA

Upstream 100 bases:

>100_bases
AAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATAGGGCGTAATCCCTGTTTAAATGGAAA
AGTAGGTGATCTTTGTGTCA

Downstream 100 bases:

>100_bases
ATGAGTAATGACAGGAATCTGAGTATTCCTGTCTTCTACATATAAATCAATGTATGTGTAACGGAAATGTAAGAGGAGGT
GCTCGTTGATGTCAGACAAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MEQKQMQENSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYEFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYIYIAQPPLFKVQQGKKIQYAYNEKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Sequences:

>Translated_640_residues
MEQKQMQENSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYEFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYIYIAQPPLFKVQQGKKIQYAYNEKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
>Mature_640_residues
MEQKQMQENSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYEFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYIYIAQPPLFKVQQGKKIQYAYNEKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913406, Length=609, Percent_Identity=27.0935960591133, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=26.3157894736842, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI48994957, Length=553, Percent_Identity=59.4936708860759, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=39.6496815286624, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17535065, Length=618, Percent_Identity=25.0809061488673, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=541, Percent_Identity=25.3234750462107, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=616, Percent_Identity=26.2987012987013, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136538, Length=620, Percent_Identity=25.1612903225806, Blast_Score=119, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_BACAN (Q9X3Y6)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- EMBL:   AF090333
- RefSeq:   NP_842577.1
- RefSeq:   YP_016608.1
- RefSeq:   YP_026296.1
- ProteinModelPortal:   Q9X3Y6
- EnsemblBacteria:   EBBACT00000010328
- EnsemblBacteria:   EBBACT00000015641
- EnsemblBacteria:   EBBACT00000021045
- GeneID:   1083919
- GeneID:   2816408
- GeneID:   2848886
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0005
- KEGG:   bar:GBAA_0005
- KEGG:   bat:BAS0005
- TIGR:   BA_0005
- TIGR:   GBAA_0005
- GeneTree:   EBGT00050000002750
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK05644
- BioCyc:   BANT260799:BAS0005-MONOMER
- BioCyc:   BANT261594:GBAA0005-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 71897; Mature: 71897

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKQMQENSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVSIEEDNSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGDTDQTGTITRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FQETTVYEFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNSILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYIYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KVQQGKKIQYAYNEKELEKILAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQKQMQENSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVSIEEDNSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGDTDQTGTITRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FQETTVYEFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNSILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYIYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KVQQGKKIQYAYNEKELEKILAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629; 10103241