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The map label for this gene is recF

Identifier: 49183043

GI number: 49183043

Start: 3419

End: 4546

Strand: Direct

Name: recF

Synonym: BAS0004

Alternate gene names: 49183043

Gene position: 3419-4546 (Clockwise)

Preceding gene: 49183042

Following gene: 49183044

Centisome position: 0.07

GC content: 34.57

Gene sequence:

>1128_bases
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAAGTAAATGT
AATTATCGGCGAGAACGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTGGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGTGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGAAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATATTGCGAAAACGCTTTGAGTTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCAGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCGATTCTTTTACTCGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Upstream 100 bases:

>100_bases
AGAACTTGAAAATAGAAGAGGGCGCAAGCTATATGCGAACGATATTATTGAAATTCCAGGAAGCGGAAGTTTCCAAGTTC
AGTCATAAAGGGGGAGCCCT

Downstream 100 bases:

>100_bases
TCCCTGTTTAAATGGAAAAGTAGGTGATCTTTGTGTCAATGGAACAAAAGCAAATGCAAGAAAATTCATATGATGAAAGT
CAAATACAGGTACTTGAAGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Sequences:

>Translated_375_residues
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA
>Mature_375_residues
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=371, Percent_Identity=25.0673854447439, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_BACAA (C3P8P8)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864661.1
- ProteinModelPortal:   C3P8P8
- EnsemblBacteria:   EBBACT00000127295
- GeneID:   7848438
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0004
- GeneTree:   EBGT00050000002258
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43363; Mature: 43363

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDREL
CCCCCCHHHCCCCCHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWDEDFGQIKGKLQKRNSSLSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPED
EEECHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQRNHLLKKMQGNSKNEETMLDV
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCHHHH
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEECCCCEEEECCCCCCHHHHHEEEHHHH
ELIYSEVKEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCE
IHVTNGTVDCEIDRA
EEEECCEEEEEECCC
>Mature Secondary Structure
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDREL
CCCCCCHHHCCCCCHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWDEDFGQIKGKLQKRNSSLSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPED
EEECHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQRNHLLKKMQGNSKNEETMLDV
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCHHHH
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEECCCCEEEECCCCCCHHHHHEEEHHHH
ELIYSEVKEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCE
IHVTNGTVDCEIDRA
EEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA