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The map label for this gene is traD [H]

Identifier: 46447075

GI number: 46447075

Start: 1714571

End: 1716241

Strand: Direct

Name: traD [H]

Synonym: pc1441

Alternate gene names: 46447075

Gene position: 1714571-1716241 (Clockwise)

Preceding gene: 46447074

Following gene: 46447076

Centisome position: 71.01

GC content: 42.91

Gene sequence:

>1671_bases
ATGAAGCTATTTCAAACTATCACCGAAGGAGGTCAATTAACCACGCACAGTGTGCGTATGTTCAAGCAGGTTACGAAAAC
CTCTTTTTTTATTACCTTTGTCTTGTGGATTATCGTTTTCCTTTACCAAATGCGGGATGTGAATACGTTTTATCTCCAAA
ATGTTTTGTACTTTGAGCAAGCACGTTTTTATCAAGTGCTTCACATTGATACGATTAAGGTAGATCCCTCTTTTGGTTTG
CAAAGGGGAAGTCAGGATGTTAAGGAATACACCACAGATCAAGTTCTTCGTCTAACTAAGCCTTATGCAGACCGCTTTTA
TCGCATCACCAAACAAAGATTTCAGCACTCTGCTTTTTGGGCGCTTGCCCCTCTTTCTGTCGTCTTTGCCATTTTCTTCT
TTCGAGGAAGACAATCCATTAAAAAACACCATCTAAAAGGAAATACCCTCTCTTCCGTCTGGTTAACACGGTGGAAACTA
AAACTATCGCGTCGCGCCTCTCCCATTTCCATCGGATCGCTTCCTCTCATCAAAGGAACAGAAACACAACACATCATGAT
TACGGGCGGAACGGGTAGTGGTAAGACGAATTGCTTACATCATCTATTAAAGTCCGTGCGTCAGCAAAAGCAACGAGCAA
TTATTGTCGATACCACAGGTCTTTTAACGGAGCGGTATTATCGCGCTGGCAAAGATATTTTGTTAAATCCCCTAGATTCG
CGGGGAGCTCCTTGGCACCCTTGGATCGAATGCATAGACAAAACTAGTTATGCGACTATGGCAGAAAGTTTCATTCCTCA
AAGTCTTTCCGATAGTGATAATTATTGGCGAACTTCAGCGCGTATCGTTTTAGGTTCTTTATTTGAACAATTAAAGGATA
TTCAAAAAAACTCGACTTTAGCTGATAAAATTTTGCGAGATCCCCTTCAAGAGCTTTGTGATTTTGTCCAAGGAACAAAA
GCCGCTTCGTTAATCGACATGAACTCAGAAAAAACGGCTGCCTCCATTCGCGCTGTGATGTCTAATTTTTTGACCTGCTT
TGAGTTATTGGCCAATACCGATCAACCGTTTTCAATTCGAGAATGGATCCAACAGGAAGATCCAATTGACAATTGGTTAT
TCATTAGTTGCAAAACGAATGAACGGGCAGCCCTCAATCCCCTACTCTCTTGTTGGCTATCTGTTGCTGTACGCAGTCTG
TTGGCCTTGCCCCCAAATTTTGAACGAAGGCTTTGGTATATCATCGATGAGCTTCCGACCTTAAATCGTTTGCGTGATTT
AGACATGTTGCTGGCCGAGAGTCGCAAATACGGAGGCTGCGCCGTTCTGGCTTTGCAAAGCCCTGCCCAGTTAGAAGCCA
TCTACGGGCAAGCTGTCGCTCAAACCTTGATCGGCAATTGTGCCACCAAAATTGTCTTTGCTGAGCAAAATCCCCTTCAT
GCAGGTCGTCTTGCCGACATGTTCGGAGAGCAAGAAATTAGAGAGTATCAAAAAGGGCTGTCTTATGGAGCCAATGACAT
TCGAGATGGGGTGAGTTTAAATCAACAAACGCGGTATCAACAATTGATTACCAAGACTGATATTCAATTTCTGTCTCGTA
ACCAAGCCTTTGTACGCTTACCTGATAATTGCCCGATTGTGCGTCTTAACGTACCTATTTTACCTAGATAA

Upstream 100 bases:

>100_bases
AAACTCTTCCCAAACGTGCCTGGAAATCAACGGAAGCCCAAACGGAAACCTCGCAAGATTACAAATTAGATGTTCCATTT
TTTTGGAAACGAGGAGAGTC

Downstream 100 bases:

>100_bases
GTTTTAGTTTTTGCATTTAGTTAATTGTCTTAATATACCACCAAGTGGTATATTAAAGATATGGAACGAACAATTATTCA
GTTATCGCCTTTTTCTCGCT

Product: putative conjugative transfer protein traD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKLFQTITEGGQLTTHSVRMFKQVTKTSFFITFVLWIIVFLYQMRDVNTFYLQNVLYFEQARFYQVLHIDTIKVDPSFGL
QRGSQDVKEYTTDQVLRLTKPYADRFYRITKQRFQHSAFWALAPLSVVFAIFFFRGRQSIKKHHLKGNTLSSVWLTRWKL
KLSRRASPISIGSLPLIKGTETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYRAGKDILLNPLDS
RGAPWHPWIECIDKTSYATMAESFIPQSLSDSDNYWRTSARIVLGSLFEQLKDIQKNSTLADKILRDPLQELCDFVQGTK
AASLIDMNSEKTAASIRAVMSNFLTCFELLANTDQPFSIREWIQQEDPIDNWLFISCKTNERAALNPLLSCWLSVAVRSL
LALPPNFERRLWYIIDELPTLNRLRDLDMLLAESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNCATKIVFAEQNPLH
AGRLADMFGEQEIREYQKGLSYGANDIRDGVSLNQQTRYQQLITKTDIQFLSRNQAFVRLPDNCPIVRLNVPILPR

Sequences:

>Translated_556_residues
MKLFQTITEGGQLTTHSVRMFKQVTKTSFFITFVLWIIVFLYQMRDVNTFYLQNVLYFEQARFYQVLHIDTIKVDPSFGL
QRGSQDVKEYTTDQVLRLTKPYADRFYRITKQRFQHSAFWALAPLSVVFAIFFFRGRQSIKKHHLKGNTLSSVWLTRWKL
KLSRRASPISIGSLPLIKGTETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYRAGKDILLNPLDS
RGAPWHPWIECIDKTSYATMAESFIPQSLSDSDNYWRTSARIVLGSLFEQLKDIQKNSTLADKILRDPLQELCDFVQGTK
AASLIDMNSEKTAASIRAVMSNFLTCFELLANTDQPFSIREWIQQEDPIDNWLFISCKTNERAALNPLLSCWLSVAVRSL
LALPPNFERRLWYIIDELPTLNRLRDLDMLLAESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNCATKIVFAEQNPLH
AGRLADMFGEQEIREYQKGLSYGANDIRDGVSLNQQTRYQQLITKTDIQFLSRNQAFVRLPDNCPIVRLNVPILPR
>Mature_556_residues
MKLFQTITEGGQLTTHSVRMFKQVTKTSFFITFVLWIIVFLYQMRDVNTFYLQNVLYFEQARFYQVLHIDTIKVDPSFGL
QRGSQDVKEYTTDQVLRLTKPYADRFYRITKQRFQHSAFWALAPLSVVFAIFFFRGRQSIKKHHLKGNTLSSVWLTRWKL
KLSRRASPISIGSLPLIKGTETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYRAGKDILLNPLDS
RGAPWHPWIECIDKTSYATMAESFIPQSLSDSDNYWRTSARIVLGSLFEQLKDIQKNSTLADKILRDPLQELCDFVQGTK
AASLIDMNSEKTAASIRAVMSNFLTCFELLANTDQPFSIREWIQQEDPIDNWLFISCKTNERAALNPLLSCWLSVAVRSL
LALPPNFERRLWYIIDELPTLNRLRDLDMLLAESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNCATKIVFAEQNPLH
AGRLADMFGEQEIREYQKGLSYGANDIRDGVSLNQQTRYQQLITKTDIQFLSRNQAFVRLPDNCPIVRLNVPILPR

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 63741; Mature: 63741

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFQTITEGGQLTTHSVRMFKQVTKTSFFITFVLWIIVFLYQMRDVNTFYLQNVLYFEQ
CCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ARFYQVLHIDTIKVDPSFGLQRGSQDVKEYTTDQVLRLTKPYADRFYRITKQRFQHSAFW
HHHEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAPLSVVFAIFFFRGRQSIKKHHLKGNTLSSVWLTRWKLKLSRRASPISIGSLPLIKGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
ETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYRAGKDILLNPLDS
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCCEECCCCC
RGAPWHPWIECIDKTSYATMAESFIPQSLSDSDNYWRTSARIVLGSLFEQLKDIQKNSTL
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ADKILRDPLQELCDFVQGTKAASLIDMNSEKTAASIRAVMSNFLTCFELLANTDQPFSIR
HHHHHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EWIQQEDPIDNWLFISCKTNERAALNPLLSCWLSVAVRSLLALPPNFERRLWYIIDELPT
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
LNRLRDLDMLLAESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNCATKIVFAEQNPLH
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
AGRLADMFGEQEIREYQKGLSYGANDIRDGVSLNQQTRYQQLITKTDIQFLSRNQAFVRL
HHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
PDNCPIVRLNVPILPR
CCCCCEEEECCCCCCC
>Mature Secondary Structure
MKLFQTITEGGQLTTHSVRMFKQVTKTSFFITFVLWIIVFLYQMRDVNTFYLQNVLYFEQ
CCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ARFYQVLHIDTIKVDPSFGLQRGSQDVKEYTTDQVLRLTKPYADRFYRITKQRFQHSAFW
HHHEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAPLSVVFAIFFFRGRQSIKKHHLKGNTLSSVWLTRWKLKLSRRASPISIGSLPLIKGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
ETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYRAGKDILLNPLDS
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCCEECCCCC
RGAPWHPWIECIDKTSYATMAESFIPQSLSDSDNYWRTSARIVLGSLFEQLKDIQKNSTL
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ADKILRDPLQELCDFVQGTKAASLIDMNSEKTAASIRAVMSNFLTCFELLANTDQPFSIR
HHHHHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EWIQQEDPIDNWLFISCKTNERAALNPLLSCWLSVAVRSLLALPPNFERRLWYIIDELPT
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
LNRLRDLDMLLAESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNCATKIVFAEQNPLH
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
AGRLADMFGEQEIREYQKGLSYGANDIRDGVSLNQQTRYQQLITKTDIQFLSRNQAFVRL
HHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
PDNCPIVRLNVPILPR
CCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]