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The map label for this gene is nuoM [H]

Identifier: 46446205

GI number: 46446205

Start: 704614

End: 706059

Strand: Direct

Name: nuoM [H]

Synonym: pc0571

Alternate gene names: 46446205

Gene position: 704614-706059 (Clockwise)

Preceding gene: 46446204

Following gene: 46446206

Centisome position: 29.18

GC content: 33.75

Gene sequence:

>1446_bases
ATGCCTAATTTGTTTTGGTTATTTTTAATACCTTTTGTCTCTTCGATTTTAATAGGAATCCTGCCAATTAATGAGAAAAA
ACATTTAAAATGGGCTTCATTGGGATTGAGTTTAATTCCTTTTTTCTTCCTTTTAGGTAACCATTTTGTTTGGATAGGAT
CTTTTTTTCAATATGAGTGGTTTGCCCTTTTATCTATTACTTTTTCACTTGAAATCGACTCTATTTCTCTTCTTTTTTTA
TATTTAACAGCAATCATCATCCCGATTGTTTTTATGAATGTAAATAGTGAAAAATTGTCAAATCCTAAGCTGTTTTATGG
ATTAATTTTATTTTTACAGGGATTATTGGGTGGTTTTTTCATGACGCGTGATCTTGTCTTTTTTACAATCTTTTGGGAGG
CGATGTTATTACCTATTTATTTTTTAATGAATATGTGGGGAGGGAGTAAACGTCATGAAGCAGCTATTAAATTTATTGTT
TATATGTTCGCAGGATCGGCTTTAATGATAGCTGCCGTATTAGCTCTTTATTTTCTTTTCGGAACTTTTGATTTAGCCCA
ATTGTCAACTCAGGAATCTACTTATCCTTATGTTAATTTAATTGCAGCAGCATTCTTATTAGCTTTTGCAGTGAAAACAC
CTCTGTTTCCTTTTCATGCTTGGCTTCCTGATACTTATTACCAAGCATCAACTTCGGGAACAATTTTATTATCGGGGTTG
TTGTCTAAAGCAGGTATTTATGGGATTATCCGCATTGGATTAGGTCTTTTTCCGCATGTAATGAAAGCGTGGAGTCCCTG
GTTACTTGGATTAGCAATAACCGGTGTTTTATATGGAGCTTTAGCAGCTTGGAGACAAAATGATTTTAAACGCTTACTTG
CCTATTCAAGTTTTTCCCATGTGAACTTTATTTTAATAGGCCTGTTTATTTTTGATCCTATTGCCCAAAAAGGAGCTATT
TTACAAGCTTTTAACCATGGAATTACCATTACGGCCCTATTTTTGGTTGCAGGCTATTTAGAAAATCGACTTGGTTTTAC
AATAATTGGAACATCCAAAGGCTTAGCTAGGTATATGCCTAACCTTTGCTGGTTGACATTCTTTTTTGTCCTCTCTTCCA
TTGCTTTACCCGGAACTAATAATTTTGTGGGTGAATTACTCATTTTTATCGGCCTTTTTGGTAAACACCCTTATTTGACT
GCTCTTACAGGCTTAACAATTGTATTTTCTGTGATATATATGCTTCGTTGGATGCAAAAAATGTATTTTGAAGAGCCAAG
TCCTTATCAAGTTCAATGGATTGATTTAACAAAAAAAGAATTTTTGATTGTCGCTCCTCTCATCTTTTTAAACTTATGGA
TTGGCTTATATCCCATGGGAATTCTCAAATATTTAGACGCTGATCATTCCAAATCGCAAGTATTGACTCAGGAGATGATA
AAATGA

Upstream 100 bases:

>100_bases
TTGTTACAGAAATTTCAAAATGGCCAAGTACGTTCATATATTGCTTGGATGGTAACAGGGGCTGGTATATTGATTGTTTA
TTTTATTATTGAGGGATTCC

Downstream 100 bases:

>100_bases
ATCCTACTTTGACATTAACCGATTTTATTTCTATCGGGCCATTATTAATTGTTTTAATGACCGCTTTAATCATTATCCTT
ATAGAAAGTTTCAGTGAGAA

Product: putative NADH-ubiquinone oxidoreductase chain M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MPNLFWLFLIPFVSSILIGILPINEKKHLKWASLGLSLIPFFFLLGNHFVWIGSFFQYEWFALLSITFSLEIDSISLLFL
YLTAIIIPIVFMNVNSEKLSNPKLFYGLILFLQGLLGGFFMTRDLVFFTIFWEAMLLPIYFLMNMWGGSKRHEAAIKFIV
YMFAGSALMIAAVLALYFLFGTFDLAQLSTQESTYPYVNLIAAAFLLAFAVKTPLFPFHAWLPDTYYQASTSGTILLSGL
LSKAGIYGIIRIGLGLFPHVMKAWSPWLLGLAITGVLYGALAAWRQNDFKRLLAYSSFSHVNFILIGLFIFDPIAQKGAI
LQAFNHGITITALFLVAGYLENRLGFTIIGTSKGLARYMPNLCWLTFFFVLSSIALPGTNNFVGELLIFIGLFGKHPYLT
ALTGLTIVFSVIYMLRWMQKMYFEEPSPYQVQWIDLTKKEFLIVAPLIFLNLWIGLYPMGILKYLDADHSKSQVLTQEMI
K

Sequences:

>Translated_481_residues
MPNLFWLFLIPFVSSILIGILPINEKKHLKWASLGLSLIPFFFLLGNHFVWIGSFFQYEWFALLSITFSLEIDSISLLFL
YLTAIIIPIVFMNVNSEKLSNPKLFYGLILFLQGLLGGFFMTRDLVFFTIFWEAMLLPIYFLMNMWGGSKRHEAAIKFIV
YMFAGSALMIAAVLALYFLFGTFDLAQLSTQESTYPYVNLIAAAFLLAFAVKTPLFPFHAWLPDTYYQASTSGTILLSGL
LSKAGIYGIIRIGLGLFPHVMKAWSPWLLGLAITGVLYGALAAWRQNDFKRLLAYSSFSHVNFILIGLFIFDPIAQKGAI
LQAFNHGITITALFLVAGYLENRLGFTIIGTSKGLARYMPNLCWLTFFFVLSSIALPGTNNFVGELLIFIGLFGKHPYLT
ALTGLTIVFSVIYMLRWMQKMYFEEPSPYQVQWIDLTKKEFLIVAPLIFLNLWIGLYPMGILKYLDADHSKSQVLTQEMI
K
>Mature_480_residues
PNLFWLFLIPFVSSILIGILPINEKKHLKWASLGLSLIPFFFLLGNHFVWIGSFFQYEWFALLSITFSLEIDSISLLFLY
LTAIIIPIVFMNVNSEKLSNPKLFYGLILFLQGLLGGFFMTRDLVFFTIFWEAMLLPIYFLMNMWGGSKRHEAAIKFIVY
MFAGSALMIAAVLALYFLFGTFDLAQLSTQESTYPYVNLIAAAFLLAFAVKTPLFPFHAWLPDTYYQASTSGTILLSGLL
SKAGIYGIIRIGLGLFPHVMKAWSPWLLGLAITGVLYGALAAWRQNDFKRLLAYSSFSHVNFILIGLFIFDPIAQKGAIL
QAFNHGITITALFLVAGYLENRLGFTIIGTSKGLARYMPNLCWLTFFFVLSSIALPGTNNFVGELLIFIGLFGKHPYLTA
LTGLTIVFSVIYMLRWMQKMYFEEPSPYQVQWIDLTKKEFLIVAPLIFLNLWIGLYPMGILKYLDADHSKSQVLTQEMIK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=327, Percent_Identity=31.4984709480122, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1788613, Length=430, Percent_Identity=33.953488372093, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788827, Length=274, Percent_Identity=29.9270072992701, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788829, Length=486, Percent_Identity=24.8971193415638, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788831, Length=332, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=357, Percent_Identity=26.890756302521, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54588; Mature: 54457

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLFWLFLIPFVSSILIGILPINEKKHLKWASLGLSLIPFFFLLGNHFVWIGSFFQYEW
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
FALLSITFSLEIDSISLLFLYLTAIIIPIVFMNVNSEKLSNPKLFYGLILFLQGLLGGFF
HHHHHEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
MTRDLVFFTIFWEAMLLPIYFLMNMWGGSKRHEAAIKFIVYMFAGSALMIAAVLALYFLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
GTFDLAQLSTQESTYPYVNLIAAAFLLAFAVKTPLFPFHAWLPDTYYQASTSGTILLSGL
HCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHCCCCCCHHHHHHH
LSKAGIYGIIRIGLGLFPHVMKAWSPWLLGLAITGVLYGALAAWRQNDFKRLLAYSSFSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VNFILIGLFIFDPIAQKGAILQAFNHGITITALFLVAGYLENRLGFTIIGTSKGLARYMP
HHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCC
NLCWLTFFFVLSSIALPGTNNFVGELLIFIGLFGKHPYLTALTGLTIVFSVIYMLRWMQK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MYFEEPSPYQVQWIDLTKKEFLIVAPLIFLNLWIGLYPMGILKYLDADHSKSQVLTQEMI
HHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
K
C
>Mature Secondary Structure 
PNLFWLFLIPFVSSILIGILPINEKKHLKWASLGLSLIPFFFLLGNHFVWIGSFFQYEW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
FALLSITFSLEIDSISLLFLYLTAIIIPIVFMNVNSEKLSNPKLFYGLILFLQGLLGGFF
HHHHHEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
MTRDLVFFTIFWEAMLLPIYFLMNMWGGSKRHEAAIKFIVYMFAGSALMIAAVLALYFLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
GTFDLAQLSTQESTYPYVNLIAAAFLLAFAVKTPLFPFHAWLPDTYYQASTSGTILLSGL
HCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHCCCCCCHHHHHHH
LSKAGIYGIIRIGLGLFPHVMKAWSPWLLGLAITGVLYGALAAWRQNDFKRLLAYSSFSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VNFILIGLFIFDPIAQKGAILQAFNHGITITALFLVAGYLENRLGFTIIGTSKGLARYMP
HHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCC
NLCWLTFFFVLSSIALPGTNNFVGELLIFIGLFGKHPYLTALTGLTIVFSVIYMLRWMQK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MYFEEPSPYQVQWIDLTKKEFLIVAPLIFLNLWIGLYPMGILKYLDADHSKSQVLTQEMI
HHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]