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The map label for this gene is thiE

Identifier: 45657775

GI number: 45657775

Start: 2313166

End: 2313810

Strand: Reverse

Name: thiE

Synonym: LIC11915

Alternate gene names: 45657775

Gene position: 2313810-2313166 (Counterclockwise)

Preceding gene: 45657777

Following gene: 45657774

Centisome position: 54.1

GC content: 35.19

Gene sequence:

>645_bases
GTGAGAGCAAACAGTTTTCCTTGGCGAGTGCCTGGAATTTATCCTATCCTCGATTTAGATTTTTGTAAAAGTAGAAATCT
GGATTATTTCAGTCTTCCGAAATTTTGGATAGAATATCCAGATCTAATTCCTTTTATACAGATTCGGGCTAAGTCAGCTT
CTATAAATGAATTAGAATTTATAGTAAAATCGTTACAGGATTTATATCCGGATTTGTTTTGGATTGTCAACGACTTCTGG
AAACAGGCTATTGAATGGAATTGTTTTGGAGCTCATGTCGGAAAAGAAGATTATGAAGCTTTGAATTTAGAAGAAAAGAA
TACACTCTTTAAATCTAAACTCTATTTGGGTACGTCCTCTCATACTCTGGAAGAAGTAAGCGAGTTGGATTCATCTCTTT
GGAACTATACTGGATTAGGCCCCATTTTTCCGACAGAGAATAAAGAGGATGCAAAGTCTCCAATTGGAACGAAAACTTTA
AATAAAATCAAGAATGGAAATTACTTACCTGTAACTGTGATCGGTGGACTCAAAGTTGAAAATTTAGATCTGATCTTAAA
GGAAGGGTCCTTTTTAATTTCGAGTATATCCATGGCTTGTTTAGAAAATGAATTTAGGACCGCCGCCACGAAATTACGGA
AATAA

Upstream 100 bases:

>100_bases
AGTTTTCAGGTTTTAGAGAAACAAAAAGGTGCATTTATGAATTCGATGTTACAAATTAAAAGATGGGATTGGATCGATTT
CAAATCGTAAGGTTGATTGA

Downstream 100 bases:

>100_bases
ATTTAGGTAAGTTTCAATTGACTCTAAGTTTATGTCGTATAGTATGCCTTTAAGATGAAAAAGCCAGGTGAAATTATACA
TCTATCCGCAAAGGATGACA

Product: thiamine phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRANSFPWRVPGIYPILDLDFCKSRNLDYFSLPKFWIEYPDLIPFIQIRAKSASINELEFIVKSLQDLYPDLFWIVNDFW
KQAIEWNCFGAHVGKEDYEALNLEEKNTLFKSKLYLGTSSHTLEEVSELDSSLWNYTGLGPIFPTENKEDAKSPIGTKTL
NKIKNGNYLPVTVIGGLKVENLDLILKEGSFLISSISMACLENEFRTAATKLRK

Sequences:

>Translated_214_residues
MRANSFPWRVPGIYPILDLDFCKSRNLDYFSLPKFWIEYPDLIPFIQIRAKSASINELEFIVKSLQDLYPDLFWIVNDFW
KQAIEWNCFGAHVGKEDYEALNLEEKNTLFKSKLYLGTSSHTLEEVSELDSSLWNYTGLGPIFPTENKEDAKSPIGTKTL
NKIKNGNYLPVTVIGGLKVENLDLILKEGSFLISSISMACLENEFRTAATKLRK
>Mature_214_residues
MRANSFPWRVPGIYPILDLDFCKSRNLDYFSLPKFWIEYPDLIPFIQIRAKSASINELEFIVKSLQDLYPDLFWIVNDFW
KQAIEWNCFGAHVGKEDYEALNLEEKNTLFKSKLYLGTSSHTLEEVSELDSSLWNYTGLGPIFPTENKEDAKSPIGTKTL
NKIKNGNYLPVTVIGGLKVENLDLILKEGSFLISSISMACLENEFRTAATKLRK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=27.0142180094787, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24557; Mature: 24557

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANSFPWRVPGIYPILDLDFCKSRNLDYFSLPKFWIEYPDLIPFIQIRAKSASINELEF
CCCCCCCCCCCCCCEEEECHHHHCCCCCEEECCHHHHCCCCCCCEEEEEECCCCHHHHHH
IVKSLQDLYPDLFWIVNDFWKQAIEWNCFGAHVGKEDYEALNLEEKNTLFKSKLYLGTSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHCCCCHHHHHHHHHEEECCCH
HTLEEVSELDSSLWNYTGLGPIFPTENKEDAKSPIGTKTLNKIKNGNYLPVTVIGGLKVE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCEEEEEEECCEEEC
NLDLILKEGSFLISSISMACLENEFRTAATKLRK
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRANSFPWRVPGIYPILDLDFCKSRNLDYFSLPKFWIEYPDLIPFIQIRAKSASINELEF
CCCCCCCCCCCCCCEEEECHHHHCCCCCEEECCHHHHCCCCCCCEEEEEECCCCHHHHHH
IVKSLQDLYPDLFWIVNDFWKQAIEWNCFGAHVGKEDYEALNLEEKNTLFKSKLYLGTSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHCCCCHHHHHHHHHEEECCCH
HTLEEVSELDSSLWNYTGLGPIFPTENKEDAKSPIGTKTLNKIKNGNYLPVTVIGGLKVE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCEEEEEEECCEEEC
NLDLILKEGSFLISSISMACLENEFRTAATKLRK
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA