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The map label for this gene is rsmE [H]

Identifier: 45657777

GI number: 45657777

Start: 2314660

End: 2315352

Strand: Reverse

Name: rsmE [H]

Synonym: LIC11917

Alternate gene names: 45657777

Gene position: 2315352-2314660 (Counterclockwise)

Preceding gene: 45657778

Following gene: 45657775

Centisome position: 54.13

GC content: 35.64

Gene sequence:

>693_bases
TTGAAAGAGGCGATTCTTTTCAGAAGAGAATGGAATGAAGAATATCCTTTTCATTTAAACAAAGAAGAAATTTCCCATTT
AAAAGCGCTTCGGATTTTTTCAGAAGATAAAAATTTAAGAGTATTGAACGGAATTGGAACCGAATGGATTTATGAGATTC
CAGGAAATTCAGTTCAAGGGATTTTCAAAAAGACGATCACTCATTTGAAATCGGAAAGGGTTTCCGCGGTTGCGACAGCT
ATTCCTAAAGGAAATCGATTGGAGTGGTTGTTACAAAAAGGAACCGAATTGGGTTTGACTCATTTTTATTTTTTAAATTT
CGAACAATCGGATCGAAAGGATTTCAATTTGAATCGGGCTCAAAAGATCGTGGAAGAGGCATCGATTCAATCAAAAAGAA
TTTTTCTTCCAGAAATAGTTTCCCCGGTTTCGTTGAAAGAATTTTTGATTTCGCAAAAGAAACAAAATCTTTCTATCTAT
CGATTGGATCCGCAAGGGGAATGGAAACTAAAACCTGAATTCTTTCAAAATGCAATTTGGATCATAGGTCCGGAGGGTGG
TTTTCGAGAGAAGGAAATTCTTTTGCTCCAAGAAAGAAATATACCTGGAATTAAGGTAGGGGATACAATTCTAAAAATAG
AGACCGCTGTAGTTTTTACGGCCTCGTTATTTCGTTATTCTACTATTTGTTGA

Upstream 100 bases:

>100_bases
CGGATCTGTTAGTGTATTAGAATATATTGAAATAGATAAGGAAGTAAAACCAGATCCTTCTCTTTTTGATCTAGCCAATT
TAAATCGATAGGGTTTTATT

Downstream 100 bases:

>100_bases
GATTGTTGAATTGAATGAGCAAATTAAGAACGTTACAGTTATTAGAACAATTACTCGTACATGTGTTGAGTGCCTGTAAA
TTTGCGTTTGTTACGGTTTC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKEAILFRREWNEEYPFHLNKEEISHLKALRIFSEDKNLRVLNGIGTEWIYEIPGNSVQGIFKKTITHLKSERVSAVATA
IPKGNRLEWLLQKGTELGLTHFYFLNFEQSDRKDFNLNRAQKIVEEASIQSKRIFLPEIVSPVSLKEFLISQKKQNLSIY
RLDPQGEWKLKPEFFQNAIWIIGPEGGFREKEILLLQERNIPGIKVGDTILKIETAVVFTASLFRYSTIC

Sequences:

>Translated_230_residues
MKEAILFRREWNEEYPFHLNKEEISHLKALRIFSEDKNLRVLNGIGTEWIYEIPGNSVQGIFKKTITHLKSERVSAVATA
IPKGNRLEWLLQKGTELGLTHFYFLNFEQSDRKDFNLNRAQKIVEEASIQSKRIFLPEIVSPVSLKEFLISQKKQNLSIY
RLDPQGEWKLKPEFFQNAIWIIGPEGGFREKEILLLQERNIPGIKVGDTILKIETAVVFTASLFRYSTIC
>Mature_230_residues
MKEAILFRREWNEEYPFHLNKEEISHLKALRIFSEDKNLRVLNGIGTEWIYEIPGNSVQGIFKKTITHLKSERVSAVATA
IPKGNRLEWLLQKGTELGLTHFYFLNFEQSDRKDFNLNRAQKIVEEASIQSKRIFLPEIVSPVSLKEFLISQKKQNLSIY
RLDPQGEWKLKPEFFQNAIWIIGPEGGFREKEILLLQERNIPGIKVGDTILKIETAVVFTASLFRYSTIC

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26767; Mature: 26767

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEAILFRREWNEEYPFHLNKEEISHLKALRIFSEDKNLRVLNGIGTEWIYEIPGNSVQG
CCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHH
IFKKTITHLKSERVSAVATAIPKGNRLEWLLQKGTELGLTHFYFLNFEQSDRKDFNLNRA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHH
QKIVEEASIQSKRIFLPEIVSPVSLKEFLISQKKQNLSIYRLDPQGEWKLKPEFFQNAIW
HHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECHHHHCCEEE
IIGPEGGFREKEILLLQERNIPGIKVGDTILKIETAVVFTASLFRYSTIC
EECCCCCCCCCEEEEEEECCCCCEEECCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEAILFRREWNEEYPFHLNKEEISHLKALRIFSEDKNLRVLNGIGTEWIYEIPGNSVQG
CCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHH
IFKKTITHLKSERVSAVATAIPKGNRLEWLLQKGTELGLTHFYFLNFEQSDRKDFNLNRA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHH
QKIVEEASIQSKRIFLPEIVSPVSLKEFLISQKKQNLSIYRLDPQGEWKLKPEFFQNAIW
HHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECHHHHCCEEE
IIGPEGGFREKEILLLQERNIPGIKVGDTILKIETAVVFTASLFRYSTIC
EECCCCCCCCCEEEEEEECCCCCEEECCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]