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The map label for this gene is ntaB

Identifier: 45657031

GI number: 45657031

Start: 1414978

End: 1415457

Strand: Direct

Name: ntaB

Synonym: LIC11147

Alternate gene names: 45657031

Gene position: 1414978-1415457 (Clockwise)

Preceding gene: 45657030

Following gene: 45657032

Centisome position: 33.08

GC content: 36.46

Gene sequence:

>480_bases
ATGAAAATTACCGACGAAGTTTTTAAAAATGCACTTTCTCATTTCCCATCAGGTGTCACAGTTATTACTTATTCTCATCT
TGGAAAACACAGCGGTCTTACCGTTAGTAGTTTTAGTTCTCTTTCTTTAGATCCTCCTTTGATTCTATTTTGTCTTCAAA
AGAATATAACGAGTCATGATCCTATTTATGACTCAGGTAAATTTGTAGTGAACATTTTAGCCCAGGGGCAGGATTCTATT
TCCAATCAATTTGCATCAGGTAAAACGGATAAACATTCTCTCATGGAGGAGCTTGGTTGTAAAACCGGCGATTTAAACGT
TCCTATTTTACCTGGAATTCTTTCTTACATTGAATGTGAAGTGGATCGATTTGTAGACGGTGGGGATCATTCTATTGTGA
TTGGAAAAGTGATTTCTGCAGGATCGGATGACGGACTCCGACCCTTATTGTATTACAGACGTAATTATTATTCAATTTAG

Upstream 100 bases:

>100_bases
AAAAATTAGAAATTTTCTGGTCATAAATTATACGTTTCTTTGTATCTTTTTAAGATTTCGATCGATTGACAGATCGTCTT
TCCATTTTAAGATAGACCTC

Downstream 100 bases:

>100_bases
AATTTTTGAATATACTTTTATTTTAAAATTATATTTTTAGTTGGCTGGTTTTTGCTACGTATTTCTTTTTTGTGTTTAGA
ATTTATATATTACCGATTCC

Product: nitrilotriacetate monooxygenase component B

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKITDEVFKNALSHFPSGVTVITYSHLGKHSGLTVSSFSSLSLDPPLILFCLQKNITSHDPIYDSGKFVVNILAQGQDSI
SNQFASGKTDKHSLMEELGCKTGDLNVPILPGILSYIECEVDRFVDGGDHSIVIGKVISAGSDDGLRPLLYYRRNYYSI

Sequences:

>Translated_159_residues
MKITDEVFKNALSHFPSGVTVITYSHLGKHSGLTVSSFSSLSLDPPLILFCLQKNITSHDPIYDSGKFVVNILAQGQDSI
SNQFASGKTDKHSLMEELGCKTGDLNVPILPGILSYIECEVDRFVDGGDHSIVIGKVISAGSDDGLRPLLYYRRNYYSI
>Mature_159_residues
MKITDEVFKNALSHFPSGVTVITYSHLGKHSGLTVSSFSSLSLDPPLILFCLQKNITSHDPIYDSGKFVVNILAQGQDSI
SNQFASGKTDKHSLMEELGCKTGDLNVPILPGILSYIECEVDRFVDGGDHSIVIGKVISAGSDDGLRPLLYYRRNYYSI

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=160, Percent_Identity=26.25, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17398; Mature: 17398

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDEVFKNALSHFPSGVTVITYSHLGKHSGLTVSSFSSLSLDPPLILFCLQKNITSHD
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCC
PIYDSGKFVVNILAQGQDSISNQFASGKTDKHSLMEELGCKTGDLNVPILPGILSYIECE
CCCCCCCEEEHEECCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VDRFVDGGDHSIVIGKVISAGSDDGLRPLLYYRRNYYSI
HHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKITDEVFKNALSHFPSGVTVITYSHLGKHSGLTVSSFSSLSLDPPLILFCLQKNITSHD
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCC
PIYDSGKFVVNILAQGQDSISNQFASGKTDKHSLMEELGCKTGDLNVPILPGILSYIECE
CCCCCCCEEEHEECCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VDRFVDGGDHSIVIGKVISAGSDDGLRPLLYYRRNYYSI
HHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]