Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is proS

Identifier: 45656749

GI number: 45656749

Start: 1033820

End: 1035550

Strand: Direct

Name: proS

Synonym: LIC10858

Alternate gene names: 45656749

Gene position: 1033820-1035550 (Clockwise)

Preceding gene: 45656748

Following gene: 45656750

Centisome position: 24.17

GC content: 39.05

Gene sequence:

>1731_bases
ATGAAAGCATCGAAATACATTCTTCCCACAGAAAAAGAAAATCCTGCGGACGCGGTTGTCGCCTCCCATCGTTTGATGAT
TCGCGCCGGTTTGGTTCGTAAGTCTTCCGCTGGTCTTTATTTTTATCTTCCTTTAGGTCTTAAGGTTCTGAAAAAAATAG
AACAGATCGTTCGTGAAGAAATGAATTCTACCGGGGCTTTGGAATTTGATCTTCCGATTTTAACTCCTTCTGATTTTTGG
GAACAAAGTGGCAGATGGTCTGCGATGGGAAAAGAAATGTTTCGTATCCAAGATAGACACGATCTTTCTTATGCTCTTGG
CCCCACACACGAAGAATCGTTTAGTTTTTTATTAAAGCCTCTTTTGAAATCTTATAAGGATCTTCCGGTGAACGTATATC
AGATCCAAACTAAATTTAGAGACGAGATCCGTCCTCGTTTTGGAGTGATTCGTTCTAGAGAGTTTATTATGAAAGATGCA
TATTCTTTTCATATCGATGACTCTTCTTTAGATGATACGTATCAAGCGATGAGAGTCGCTTACAGAAAAATTTTCGATCG
TTGTGGGCTGAAAACCATTCCGGTTCAAGCAGATTCCGGTAGTATGGGAGGTTCTGCGTCGGAAGAGTTTATGGTAGTTT
CTCCAATTGGAGAAGAAACATTACTTTTATGTAATTCTTGTGGTTATAGTTCCAACAGCGAAAAAACCCCTCTTATATTA
AAAAAAGAAAATGGTTCCGCGAAATTTTCCGAAAAGAAAGAAATTTCCACTCCTGGTAAGAAGACGATTTCGGAAGTTAG
TACTCTGTTAGGCGTTTCTGAATCAGAGACGATCAAGGCGGTTGCTCTTAAATCGGAGAAAAAAAAGATTCTCGTTTTTC
TTCGAGGAGATTTGGAACTCAATCTTCATAAACTTCATTCTCTCTTGAAGATTGCGGATTCGGAGCCGATGACAGACTTG
GAAATTCGTGAGCTCGGTTTGATTCCTGGGTTTATTTCTCCGATTGCGCCTAACGATAAAATTAAGGTGTTATACGATCG
TTCCTTACAAAAAGATTTTCCTTATGTGGTTGGTTCGTCCAAAGAAGATTTTCACACTCAGGGTTTTATTTTAGAAAAGG
AAATTTCTGGTCTTCCCGAATTTGCGGATGTCGCATTAGCAAGAGAAGGAGATCTTTGTCCCAATTGTAGTTCCCCCTTA
AAAGCGGAAAAAGGGATCGAAGTAGGACATATTTTCAAACTTGGAGATAAATATACAAAAGCTTTTGGTATCCAGGTTTT
GGATCAAAATGGTAAATCTAAAACTCTTACGACGGGTTGTTACGGTATCGGAGTCAATCGTACGATGGCTACCGTCATTG
AACAGTGTAACGATGAAAAAGGTATTTTTTGGCCGATCAGCATCGCTCCGTTTGAAGTCTCCCTGGTGAGTATTGTCAAA
GGAGAAGACCAGTATTCTAAAATAGAAGAATTTTATAATGTTCTAATAAATGAGGGGATAGAAGTTTTTTGGGACGATCG
AGACCTTGGTCCTGGTTTTAAACTCAAGGATTCTGAATTGATTGGTTTTCCGATTCGAATTACCATTGGTAAAAAATTCT
TCGAAAGTGGTGAGATTTCGATCTACAATCGTAAGAAGGATCAAGAAGATTCCTTTGTTTTTTCCGGTTTTGATGATTTG
GTCGCAAGAGTAGAATCTATGCGCCAAGAACTCTTTACGGAATTGAGGTAG

Upstream 100 bases:

>100_bases
GCAAAGTGATCGAGTCTATTTTTAGAATCGGATTTTTATTCCTACTCGGACTTGGGCTCTACGTCACCTTCAACGATGTA
ATGCGAATTTTCTAAAACGC

Downstream 100 bases:

>100_bases
TTATGGGAAAGGAAAATCAACAAGGCTACTTTGGAGAATTTGGCGGTCGTTATTCTCCTGAAATTCTTCACGATGCTCTC
GTAGAACTTGAAACGACTTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKASKYILPTEKENPADAVVASHRLMIRAGLVRKSSAGLYFYLPLGLKVLKKIEQIVREEMNSTGALEFDLPILTPSDFW
EQSGRWSAMGKEMFRIQDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDSSLDDTYQAMRVAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLIL
KKENGSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLELNLHKLHSLLKIADSEPMTDL
EIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSSKEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSSPL
KAEKGIEVGHIFKLGDKYTKAFGIQVLDQNGKSKTLTTGCYGIGVNRTMATVIEQCNDEKGIFWPISIAPFEVSLVSIVK
GEDQYSKIEEFYNVLINEGIEVFWDDRDLGPGFKLKDSELIGFPIRITIGKKFFESGEISIYNRKKDQEDSFVFSGFDDL
VARVESMRQELFTELR

Sequences:

>Translated_576_residues
MKASKYILPTEKENPADAVVASHRLMIRAGLVRKSSAGLYFYLPLGLKVLKKIEQIVREEMNSTGALEFDLPILTPSDFW
EQSGRWSAMGKEMFRIQDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDSSLDDTYQAMRVAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLIL
KKENGSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLELNLHKLHSLLKIADSEPMTDL
EIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSSKEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSSPL
KAEKGIEVGHIFKLGDKYTKAFGIQVLDQNGKSKTLTTGCYGIGVNRTMATVIEQCNDEKGIFWPISIAPFEVSLVSIVK
GEDQYSKIEEFYNVLINEGIEVFWDDRDLGPGFKLKDSELIGFPIRITIGKKFFESGEISIYNRKKDQEDSFVFSGFDDL
VARVESMRQELFTELR
>Mature_576_residues
MKASKYILPTEKENPADAVVASHRLMIRAGLVRKSSAGLYFYLPLGLKVLKKIEQIVREEMNSTGALEFDLPILTPSDFW
EQSGRWSAMGKEMFRIQDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDSSLDDTYQAMRVAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLIL
KKENGSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLELNLHKLHSLLKIADSEPMTDL
EIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSSKEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSSPL
KAEKGIEVGHIFKLGDKYTKAFGIQVLDQNGKSKTLTTGCYGIGVNRTMATVIEQCNDEKGIFWPISIAPFEVSLVSIVK
GEDQYSKIEEFYNVLINEGIEVFWDDRDLGPGFKLKDSELIGFPIRITIGKKFFESGEISIYNRKKDQEDSFVFSGFDDL
VARVESMRQELFTELR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=42.0091324200913, Blast_Score=202, Evalue=6e-52,
Organism=Escherichia coli, GI1786392, Length=566, Percent_Identity=43.2862190812721, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI115532348, Length=232, Percent_Identity=38.7931034482759, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=99, Percent_Identity=35.3535353535354, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=543, Percent_Identity=29.8342541436464, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24656200, Length=243, Percent_Identity=37.4485596707819, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_LEPIC (Q72U06)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000835.1
- ProteinModelPortal:   Q72U06
- SMR:   Q72U06
- GeneID:   2770123
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10858
- NMPDR:   fig|267671.1.peg.835
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   LINT267671:LIC_10858-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64844; Mature: 64844

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKASKYILPTEKENPADAVVASHRLMIRAGLVRKSSAGLYFYLPLGLKVLKKIEQIVREE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEECHHHHHHHHHHHHHHHH
MNSTGALEFDLPILTPSDFWEQSGRWSAMGKEMFRIQDRHDLSYALGPTHEESFSFLLKP
CCCCCCEEEECEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHH
LLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDAYSFHIDDSSLDDTYQAMRVA
HHHHHCCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
YRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLIL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
KKENGSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLEL
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCEE
NLHKLHSLLKIADSEPMTDLEIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSS
CHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCC
KEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSSPLKAEKGIEVGHIFKLGDKYTK
CCCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEECCCHHHH
AFGIQVLDQNGKSKTLTTGCYGIGVNRTMATVIEQCNDEKGIFWPISIAPFEVSLVSIVK
HHCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEEC
GEDQYSKIEEFYNVLINEGIEVFWDDRDLGPGFKLKDSELIGFPIRITIGKKFFESGEIS
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECHHHHCCCCEE
IYNRKKDQEDSFVFSGFDDLVARVESMRQELFTELR
EEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASKYILPTEKENPADAVVASHRLMIRAGLVRKSSAGLYFYLPLGLKVLKKIEQIVREE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEECHHHHHHHHHHHHHHHH
MNSTGALEFDLPILTPSDFWEQSGRWSAMGKEMFRIQDRHDLSYALGPTHEESFSFLLKP
CCCCCCEEEECEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHH
LLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDAYSFHIDDSSLDDTYQAMRVA
HHHHHCCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
YRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLIL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
KKENGSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLEL
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCEE
NLHKLHSLLKIADSEPMTDLEIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSS
CHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCC
KEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSSPLKAEKGIEVGHIFKLGDKYTK
CCCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEECCCHHHH
AFGIQVLDQNGKSKTLTTGCYGIGVNRTMATVIEQCNDEKGIFWPISIAPFEVSLVSIVK
HHCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEEC
GEDQYSKIEEFYNVLINEGIEVFWDDRDLGPGFKLKDSELIGFPIRITIGKKFFESGEIS
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECHHHHCCCCEE
IYNRKKDQEDSFVFSGFDDLVARVESMRQELFTELR
EEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA