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The map label for this gene is ogt

Identifier: 41408543

GI number: 41408543

Start: 2746448

End: 2746945

Strand: Direct

Name: ogt

Synonym: MAP2445

Alternate gene names: 41408543

Gene position: 2746448-2746945 (Clockwise)

Preceding gene: 41408541

Following gene: 41408546

Centisome position: 56.86

GC content: 67.67

Gene sequence:

>498_bases
ATGATCGAGTACCGCACCGTCGATAGCCCGATCGGACTGCTGACCCTGGCCGGGCGCGATCCGGTTCTGACGAACTTACG
GATGGTCGACCAGACCTACGAACCGAGCCGCACCGGGTGGACGGAGAATCCGCGGGCGTTCGCCGGGGCCGTCGAGCAAC
TCGGTGCCTATTTCGCCGGCGAGCTCACCGAGTTCGACATCGAGCTGGATCTGCGCGGCTCGGAATTCCAGCGCCGGGTG
TGGCGGGCGCTGCAGACCATCCCCTACGGGGAAACCAGGTCGTACGGCGAAATCGCCGAGCAGATCGGCGCGCCCGGCGC
CGCGCGCGCCGTCGGATTGGCCAACGGGCACAACCCGATCGCGATCGTGGTGCCCTGCCATCGCGTCATCGGCGCCAGCG
GGAAGCTGACCGGATACGGCGGCGGACTGGACCGCAAACAAACGCTGCTGGCACTGGAGAGGCGCCACTCGCCGGCGAGC
CTGACGCTATTCGACTGA

Upstream 100 bases:

>100_bases
CCAACACAGCAACCACATGAAAGCCATACCCGACGTCCCTGGGCAATTCGAAGCCTAAGGCCGACGGCCCCGAACACCCT
TCAAGAAAGGTAAGGACAGC

Downstream 100 bases:

>100_bases
GCGGGTGCCGGGCCGGTAAAACTGGCCAGCATAAGGATTTTCGGTTTCATTCACCGCGGTTGCCCTCGGTCAAGTCCGCG
AGGAATCGCGGCAGGCGACC

Product: Ogt

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIEYRTVDSPIGLLTLAGRDPVLTNLRMVDQTYEPSRTGWTENPRAFAGAVEQLGAYFAGELTEFDIELDLRGSEFQRRV
WRALQTIPYGETRSYGEIAEQIGAPGAARAVGLANGHNPIAIVVPCHRVIGASGKLTGYGGGLDRKQTLLALERRHSPAS
LTLFD

Sequences:

>Translated_165_residues
MIEYRTVDSPIGLLTLAGRDPVLTNLRMVDQTYEPSRTGWTENPRAFAGAVEQLGAYFAGELTEFDIELDLRGSEFQRRV
WRALQTIPYGETRSYGEIAEQIGAPGAARAVGLANGHNPIAIVVPCHRVIGASGKLTGYGGGLDRKQTLLALERRHSPAS
LTLFD
>Mature_165_residues
MIEYRTVDSPIGLLTLAGRDPVLTNLRMVDQTYEPSRTGWTENPRAFAGAVEQLGAYFAGELTEFDIELDLRGSEFQRRV
WRALQTIPYGETRSYGEIAEQIGAPGAARAVGLANGHNPIAIVVPCHRVIGASGKLTGYGGGLDRKQTLLALERRHSPAS
LTLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=33.7349397590361, Blast_Score=85, Evalue=3e-17,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=56.6037735849057, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1788542, Length=107, Percent_Identity=45.7943925233645, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=78, Percent_Identity=52.5641025641026, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=51.25, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=47.1264367816092, Blast_Score=93, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=150, Percent_Identity=34.6666666666667, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_MYCPA (Q9ZET8)

Other databases:

- EMBL:   AJ011838
- EMBL:   AE016958
- RefSeq:   NP_961379.1
- ProteinModelPortal:   Q9ZET8
- SMR:   Q9ZET8
- EnsemblBacteria:   EBMYCT00000036314
- GeneID:   2718320
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2445
- NMPDR:   fig|262316.1.peg.2445
- GeneTree:   EBGT00050000016345
- HOGENOM:   HBG752398
- OMA:   THARAVG
- ProtClustDB:   CLSK791095
- BRENDA:   2.1.1.63
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 17999; Mature: 17999

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEYRTVDSPIGLLTLAGRDPVLTNLRMVDQTYEPSRTGWTENPRAFAGAVEQLGAYFAG
CCCEECCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
ELTEFDIELDLRGSEFQRRVWRALQTIPYGETRSYGEIAEQIGAPGAARAVGLANGHNPI
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIVVPCHRVIGASGKLTGYGGGLDRKQTLLALERRHSPASLTLFD
EEEEECHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MIEYRTVDSPIGLLTLAGRDPVLTNLRMVDQTYEPSRTGWTENPRAFAGAVEQLGAYFAG
CCCEECCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
ELTEFDIELDLRGSEFQRRVWRALQTIPYGETRSYGEIAEQIGAPGAARAVGLANGHNPI
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIVVPCHRVIGASGKLTGYGGGLDRKQTLLALERRHSPASLTLFD
EEEEECHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA