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The map label for this gene is alkA

Identifier: 41408541

GI number: 41408541

Start: 2743500

End: 2745023

Strand: Direct

Name: alkA

Synonym: MAP2443

Alternate gene names: 41408541

Gene position: 2743500-2745023 (Clockwise)

Preceding gene: 41408540

Following gene: 41408543

Centisome position: 56.8

GC content: 71.85

Gene sequence:

>1524_bases
GTGCACGACGATTTCGAACGCTGCTACCGCGCCGTCCAGTCCAAGGATGCGCGGTTCGACGGCTGGTTCGTCACCGCCGT
GCTGACCACCGGAATCTACTGCCGGCCCAGTTGCCCGGTGCGCCCGCCGTTCGCCCGTAATGTGCGGTTTTATCCGACCG
CGGCGGCCGCTCAGCGCGCCGGCTTCCGGGCCTGCAAGCGGTGCCGTCCCGACGCCTCGCCGGGATCGCCGGAATGGAAC
GTGCGCGGCGACGTGGTGGCGCGCACGATGCGGCTGATCGCCGACGGCACGGTGGACCGCGACGGTGTCGGCGGCCTGGC
CGCCCGGCTGGGCTACACCACCCGGCAGCTGGAGCGGTTGCTGCAGGCGGAGGTCGGCGCCGGTCCGCTCGCGCTGGCCC
GGGCGCAACGCGCCCAGACGGCGCGGGTGCTGATCGAGACCACGGAGCTGCCCTTCGGCGACGTGGCATTCGCCGCCGGC
TTCTCCAGCATTCGTCAGTTCAACGACACCGTGCGCGCGGTGTTCGAAAGCCCGCCGAGCGTGCTGCGCCGGCGCGCCTC
GGCGCGCTGCGCATCCGCGGCCAATTCGCCTGGCGCCGTGTGCCTTCGGCTGCCGGTCCGCACACCCTTCGGCTTCCATG
GCGTCTTCGGTCACCTCGCCGCGAGCGTCGTTCCCGGGTGCGAGGAGGTCCGCGACGGCGCCTACCGACGCACGCTGCGG
CTGGGGTTCGGCACCGGCATCGTCACGTTGACCCCCGCCGCCGACCACGTCCGTTGCGAGCTGGTGCTCGACGACTTCCG
GGACCTGACGGCGGCGATCGCCCGTTGCCGGCGGCTGCTGGATCTCGACGCCGACCCCGAAGCGGTGGACGAGGCGCTGG
CCGCCGACCCGCAGCTCGCCCCGGCGGTGAGAAAGGCGCCCGGCCAATGCATTCCGCGCACCGTCGACGAGGCCGAGCTC
GCGGTACGCGCCGTGCTGGGCCAGCAGGTGTCCATCCGGGCGGCGCGCACCCATGCGGGGCGTTTGGTCGCCGCCTACGG
CCGGGCGGTCCACGACCCCGAGGGAACCTTGACCCACACCTTCCCGTCGGTGCAGCAGCTGGCCGACGTCGACCCCATCC
ATCTGGCGGTGCCCAAGGCCCGGCAGCGCACGCTGGCCGCGCTGGTCGCCGGCCTCGCCGACCGCAGCATCGTCCTGGAT
ACCGGGTGCGACTGGCAAAGCGCCCGAACGCAATTGCTGGCATTACCGGGAGTCGGCCCGTGGACCGCGGAAGTCATTGC
GATGCGCGGCCTGGGCGATCCCGATGCGTTCCCGGCCGCCGACCTGGGTCTGCGCGTCGCGGCCAAACGGCTCGGATTGC
CGTCGGGCCAGCGGTCGCTGACCGCTGCCAGCGCACGCTGGCGGCCCTGGCGTTCCTATGCCACGCAATACCTTTGGACC
ACTCTCGAGCATCCGGTGAACCATTGGCCACCGCAGCAGCCATCGAAGGGGATTCTCAATGACGTTGTCAAGCCGCCGCG
GTAG

Upstream 100 bases:

>100_bases
GCGGCGGCTGGTAGCCGGCCGCCGATCCCGCTCCACGCCCGGCGACGACGATGTCGCTTTTCCGCTAGTTTTGTCGGCGG
GCGGGTGTAAGTCTTGAGGC

Downstream 100 bases:

>100_bases
TGGTTGCGGGGTGGTAGACAGCGTGGTCGCGCAGCATGGCCCACAGGACGTTGAGGCGGCGGCGGGCCAGGGCGAGGACG
GCTTGGGTGTGGCGTTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MHDDFERCYRAVQSKDARFDGWFVTAVLTTGIYCRPSCPVRPPFARNVRFYPTAAAAQRAGFRACKRCRPDASPGSPEWN
VRGDVVARTMRLIADGTVDRDGVGGLAARLGYTTRQLERLLQAEVGAGPLALARAQRAQTARVLIETTELPFGDVAFAAG
FSSIRQFNDTVRAVFESPPSVLRRRASARCASAANSPGAVCLRLPVRTPFGFHGVFGHLAASVVPGCEEVRDGAYRRTLR
LGFGTGIVTLTPAADHVRCELVLDDFRDLTAAIARCRRLLDLDADPEAVDEALAADPQLAPAVRKAPGQCIPRTVDEAEL
AVRAVLGQQVSIRAARTHAGRLVAAYGRAVHDPEGTLTHTFPSVQQLADVDPIHLAVPKARQRTLAALVAGLADRSIVLD
TGCDWQSARTQLLALPGVGPWTAEVIAMRGLGDPDAFPAADLGLRVAAKRLGLPSGQRSLTAASARWRPWRSYATQYLWT
TLEHPVNHWPPQQPSKGILNDVVKPPR

Sequences:

>Translated_507_residues
MHDDFERCYRAVQSKDARFDGWFVTAVLTTGIYCRPSCPVRPPFARNVRFYPTAAAAQRAGFRACKRCRPDASPGSPEWN
VRGDVVARTMRLIADGTVDRDGVGGLAARLGYTTRQLERLLQAEVGAGPLALARAQRAQTARVLIETTELPFGDVAFAAG
FSSIRQFNDTVRAVFESPPSVLRRRASARCASAANSPGAVCLRLPVRTPFGFHGVFGHLAASVVPGCEEVRDGAYRRTLR
LGFGTGIVTLTPAADHVRCELVLDDFRDLTAAIARCRRLLDLDADPEAVDEALAADPQLAPAVRKAPGQCIPRTVDEAEL
AVRAVLGQQVSIRAARTHAGRLVAAYGRAVHDPEGTLTHTFPSVQQLADVDPIHLAVPKARQRTLAALVAGLADRSIVLD
TGCDWQSARTQLLALPGVGPWTAEVIAMRGLGDPDAFPAADLGLRVAAKRLGLPSGQRSLTAASARWRPWRSYATQYLWT
TLEHPVNHWPPQQPSKGILNDVVKPPR
>Mature_507_residues
MHDDFERCYRAVQSKDARFDGWFVTAVLTTGIYCRPSCPVRPPFARNVRFYPTAAAAQRAGFRACKRCRPDASPGSPEWN
VRGDVVARTMRLIADGTVDRDGVGGLAARLGYTTRQLERLLQAEVGAGPLALARAQRAQTARVLIETTELPFGDVAFAAG
FSSIRQFNDTVRAVFESPPSVLRRRASARCASAANSPGAVCLRLPVRTPFGFHGVFGHLAASVVPGCEEVRDGAYRRTLR
LGFGTGIVTLTPAADHVRCELVLDDFRDLTAAIARCRRLLDLDADPEAVDEALAADPQLAPAVRKAPGQCIPRTVDEAEL
AVRAVLGQQVSIRAARTHAGRLVAAYGRAVHDPEGTLTHTFPSVQQLADVDPIHLAVPKARQRTLAALVAGLADRSIVLD
TGCDWQSARTQLLALPGVGPWTAEVIAMRGLGDPDAFPAADLGLRVAAKRLGLPSGQRSLTAASARWRPWRSYATQYLWT
TLEHPVNHWPPQQPSKGILNDVVKPPR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=281, Percent_Identity=33.0960854092527, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=33.7349397590361, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 54836; Mature: 54836

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDFERCYRAVQSKDARFDGWFVTAVLTTGIYCRPSCPVRPPFARNVRFYPTAAAAQRA
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEECCCCCCCCCHHCCEEECCCHHHHHHH
GFRACKRCRPDASPGSPEWNVRGDVVARTMRLIADGTVDRDGVGGLAARLGYTTRQLERL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHH
LQAEVGAGPLALARAQRAQTARVLIETTELPFGDVAFAAGFSSIRQFNDTVRAVFESPPS
HHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLRRRASARCASAANSPGAVCLRLPVRTPFGFHGVFGHLAASVVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHEE
LGFGTGIVTLTPAADHVRCELVLDDFRDLTAAIARCRRLLDLDADPEAVDEALAADPQLA
EECCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PAVRKAPGQCIPRTVDEAELAVRAVLGQQVSIRAARTHAGRLVAAYGRAVHDPEGTLTHT
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHCCCCCCEEEC
FPSVQQLADVDPIHLAVPKARQRTLAALVAGLADRSIVLDTGCDWQSARTQLLALPGVGP
CHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCEEEECCCCCC
WTAEVIAMRGLGDPDAFPAADLGLRVAAKRLGLPSGQRSLTAASARWRPWRSYATQYLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
TLEHPVNHWPPQQPSKGILNDVVKPPR
HHHHHHHCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MHDDFERCYRAVQSKDARFDGWFVTAVLTTGIYCRPSCPVRPPFARNVRFYPTAAAAQRA
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEECCCCCCCCCHHCCEEECCCHHHHHHH
GFRACKRCRPDASPGSPEWNVRGDVVARTMRLIADGTVDRDGVGGLAARLGYTTRQLERL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHH
LQAEVGAGPLALARAQRAQTARVLIETTELPFGDVAFAAGFSSIRQFNDTVRAVFESPPS
HHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLRRRASARCASAANSPGAVCLRLPVRTPFGFHGVFGHLAASVVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHEE
LGFGTGIVTLTPAADHVRCELVLDDFRDLTAAIARCRRLLDLDADPEAVDEALAADPQLA
EECCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PAVRKAPGQCIPRTVDEAELAVRAVLGQQVSIRAARTHAGRLVAAYGRAVHDPEGTLTHT
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHCCCCCCEEEC
FPSVQQLADVDPIHLAVPKARQRTLAALVAGLADRSIVLDTGCDWQSARTQLLALPGVGP
CHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCEEEECCCCCC
WTAEVIAMRGLGDPDAFPAADLGLRVAAKRLGLPSGQRSLTAASARWRPWRSYATQYLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
TLEHPVNHWPPQQPSKGILNDVVKPPR
HHHHHHHCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]