BacMap

Definition	Mycobacterium avium subsp. paratuberculosis K-10, complete genome.
Accession	NC_002944
Length	4,829,781

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The map label for this gene is hdhA [C]

Identifier: 41407479

GI number: 41407479

Start: 1493035

End: 1493805

Strand: Direct

Name: hdhA [C]

Synonym: MAP1381

Alternate gene names: 41407479

Gene position: 1493035-1493805 (Clockwise)

Preceding gene: 41407478

Following gene: 41407486

Centisome position: 30.91

GC content: 64.72

Gene sequence:

>771_bases
GTGCGCGGATTACAGGGCAAGACGTTCATCGTTGCGGGCGGATCGACCGGAATCGGAGCCGCAACCGCCGAACGCCTCGC
CAGCGAGGGAGCAGCCGTCACCGTCGGTGACATCAACATCGAAGGCGCCAACGCGACCGTCGGGCGGATCACGCAATCCG
GCGGGCGCGCGATCGCGGTGGAATTCGACCTTGCCGACGAAGCATCCGTTCGGAACCTCGTGCAGAAGACCATTGGCGAG
TTCGGCGCCCTCCACGGCCTCCACAACGTCGGCTCAGATCTATCCGCCGAGAACCTCGGCCGCGACACCACGCTGTTGGA
CACCGATTTCGATGTCTGGCGCCGCACGCTCGACGTCAACCTCCTGGGTTACGTGCGCACCAGTCGCGCCGTCTTGGCGC
ATCTCCTCCAGCAAGGCAGCGGAAGCATCGTAAACACGTCCTCGGGAGGCTCGCTAGGCACCGACCCGATGCACGTCGCC
TACAACGCCGCCAAAGCCGCCGTCAACCAACTCACCCGCCACATCGCGAACAACTGGGGCGCGCAAGGGGTCCGGTGCAA
CGGCGTCATGCCCGGCCTGGTCATGGGCGAAACCCAGAAGCAGCAGAACGACATTCAGTTGCAGCAGATGTTCCTGCAGG
CCGCCAAGGTCACCCGCCTCGGAGAACCGCGCGACATCGCCGCGATCACTGCCTTCCTTCTCTCCGATGAGGCCGAATGG
ATCAACGGGCAGGTGTGGTACATCGGCGGCGCCTCACACATGCGCCAGTAG

Upstream 100 bases:

>100_bases
CGCGGTGGCCTGGCTCGCATCGGATCAATCGAGATTCGTTACCGCATCCGGTATCTCGGTCGACCTGGGTGTCGCCTACT
GCTAGCGAGAGGATGGGCCC

Downstream 100 bases:

>100_bases
CAGGATTGGTATTCCCGCGAACGCGGCGTCGATGCCCTCAATTGTCGGCCCCGCACTCGAATTCACGAAATGCGTGGGCC
CACAGGTCGTGACGAAGCCA

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRGLQGKTFIVAGGSTGIGAATAERLASEGAAVTVGDINIEGANATVGRITQSGGRAIAVEFDLADEASVRNLVQKTIGE
FGALHGLHNVGSDLSAENLGRDTTLLDTDFDVWRRTLDVNLLGYVRTSRAVLAHLLQQGSGSIVNTSSGGSLGTDPMHVA
YNAAKAAVNQLTRHIANNWGAQGVRCNGVMPGLVMGETQKQQNDIQLQQMFLQAAKVTRLGEPRDIAAITAFLLSDEAEW
INGQVWYIGGASHMRQ

Sequences:

>Translated_256_residues
MRGLQGKTFIVAGGSTGIGAATAERLASEGAAVTVGDINIEGANATVGRITQSGGRAIAVEFDLADEASVRNLVQKTIGE
FGALHGLHNVGSDLSAENLGRDTTLLDTDFDVWRRTLDVNLLGYVRTSRAVLAHLLQQGSGSIVNTSSGGSLGTDPMHVA
YNAAKAAVNQLTRHIANNWGAQGVRCNGVMPGLVMGETQKQQNDIQLQQMFLQAAKVTRLGEPRDIAAITAFLLSDEAEW
INGQVWYIGGASHMRQ
>Mature_256_residues
MRGLQGKTFIVAGGSTGIGAATAERLASEGAAVTVGDINIEGANATVGRITQSGGRAIAVEFDLADEASVRNLVQKTIGE
FGALHGLHNVGSDLSAENLGRDTTLLDTDFDVWRRTLDVNLLGYVRTSRAVLAHLLQQGSGSIVNTSSGGSLGTDPMHVA
YNAAKAAVNQLTRHIANNWGAQGVRCNGVMPGLVMGETQKQQNDIQLQQMFLQAAKVTRLGEPRDIAAITAFLLSDEAEW
INGQVWYIGGASHMRQ

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=252, Percent_Identity=29.7619047619048, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=27.734375, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=31.1475409836066, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=30.0380228136882, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=27.7566539923954, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI5031737, Length=256, Percent_Identity=25.390625, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI66933014, Length=153, Percent_Identity=31.3725490196078, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI83715985, Length=263, Percent_Identity=27.7566539923954, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4503301, Length=256, Percent_Identity=26.953125, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31542939, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI7706318, Length=192, Percent_Identity=25.5208333333333, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=31.0483870967742, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082100, Length=267, Percent_Identity=30.7116104868914, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=29.1338582677165, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=25.9842519685039, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=28.4615384615385, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=31.3008130081301, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=28.9156626506024, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082160, Length=255, Percent_Identity=25.0980392156863, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788459, Length=248, Percent_Identity=26.6129032258064, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786812, Length=260, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=28.030303030303, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=28.1124497991968, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=32.5757575757576, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=30.03663003663, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=28.1481481481481, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=31.0606060606061, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=273, Percent_Identity=30.4029304029304, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=245, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=26.4591439688716, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=29.277566539924, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=29.0836653386454, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=27.9527559055118, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=264, Percent_Identity=31.0606060606061, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536651, Length=262, Percent_Identity=26.7175572519084, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=29.277566539924, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=269, Percent_Identity=30.4832713754647, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=30.2681992337165, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=262, Percent_Identity=27.8625954198473, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=29.5880149812734, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=271, Percent_Identity=29.520295202952, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=246, Percent_Identity=28.8617886178862, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=270, Percent_Identity=29.2592592592593, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=262, Percent_Identity=29.3893129770992, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=259, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=27.8810408921933, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=258, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=167, Percent_Identity=28.7425149700599, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=256, Percent_Identity=27.734375, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=27.2030651340996, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=26.3959390862944, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357041, Length=259, Percent_Identity=33.976833976834, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=32.156862745098, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=30.3846153846154, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28571526, Length=259, Percent_Identity=30.5019305019305, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=27.3809523809524, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24644337, Length=258, Percent_Identity=32.1705426356589, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17737361, Length=265, Percent_Identity=29.4339622641509, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21357673, Length=198, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859837, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27039; Mature: 27039

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLQGKTFIVAGGSTGIGAATAERLASEGAAVTVGDINIEGANATVGRITQSGGRAIAV
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECEEECCCCCHHHHEECCCCCEEEE
EFDLADEASVRNLVQKTIGEFGALHGLHNVGSDLSAENLGRDTTLLDTDFDVWRRTLDVN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCHHHHHHHHCCH
LLGYVRTSRAVLAHLLQQGSGSIVNTSSGGSLGTDPMHVAYNAAKAAVNQLTRHIANNWG
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
AQGVRCNGVMPGLVMGETQKQQNDIQLQQMFLQAAKVTRLGEPRDIAAITAFLLSDEAEW
CCCEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
INGQVWYIGGASHMRQ
HCCEEEEEECHHHHCC
>Mature Secondary Structure
MRGLQGKTFIVAGGSTGIGAATAERLASEGAAVTVGDINIEGANATVGRITQSGGRAIAV
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECEEECCCCCHHHHEECCCCCEEEE
EFDLADEASVRNLVQKTIGEFGALHGLHNVGSDLSAENLGRDTTLLDTDFDVWRRTLDVN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCHHHHHHHHCCH
LLGYVRTSRAVLAHLLQQGSGSIVNTSSGGSLGTDPMHVAYNAAKAAVNQLTRHIANNWG
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
AQGVRCNGVMPGLVMGETQKQQNDIQLQQMFLQAAKVTRLGEPRDIAAITAFLLSDEAEW
CCCEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
INGQVWYIGGASHMRQ
HCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]