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The map label for this gene is leuB [H]

Identifier: 39997970

GI number: 39997970

Start: 3154503

End: 3155591

Strand: Reverse

Name: leuB [H]

Synonym: GSU2879

Alternate gene names: 39997970

Gene position: 3155591-3154503 (Counterclockwise)

Preceding gene: 39997971

Following gene: 39997969

Centisome position: 82.73

GC content: 64.55

Gene sequence:

>1089_bases
ATGGCTCAGGTGTTCAAGGTGGCGGTTCTTCCCGGCGACGGCATCGGTCCCGAGGTGATGGCCGAGGCGCTCCGGGTGCT
GGATGCCGTGGAGGCAAAATACGACGTGAAGTTCGAGCGGACCCACGCCAACGTGGGTGGCGCCGGCATTGATATCGAGG
GGAAAGCTCTCCCCGAAACCACCGTGAACATCTGCAAGGCTGCCGACGCCATCCTCTTCGGCTCCGTGGGCGGACCCAAG
TGGGAGTCGCTCCCTCCGGACGAGCAGCCCGAGCGGGGCGCGCTCCTGCCGCTGCGGAAGATCTTCGGTCTCTACGCCAA
CCTGCGGCCGGCCATCATTTTCCCGTCCCTCACCGGCGCCTCCTCCCTCAAGGAAGAGGTAATAGCCGGGGGCTTCAACG
TGCTGGTCATCCGGGAGCTCACCGGCGGCATCTACTTTGCCCAGCCCAAGGGGATCGAAGGCGAAGGGCGCGACCGGGTC
GGCTTCGACACCATGCGCTACAGCGTGCCCGAGATCGAGCGGATAACCCATGTGGCCTTCCAGGCTGCCCGCAAGCGGGG
CAAGAAGGTCTGCTCCATCGACAAGGCCAACGTCCTCTCCTCGTCGGTTCTCTGGCGCGAGGTGGTCACCGGCATTGCCA
AGGAATACCCGGACGTGGAACTCTCCCACATGTACGTGGACAACGCCGCCATGCAACTGGTCCGCTGGCCCAAGCAGTTC
GACGTGATCCTGTGCGAGAACATGTTCGGCGACATCCTCTCGGACGAGGCGGCCATGCTGACCGGCTCCCTCGGGATGCT
GCCTTCCGCGTCCCTGGCCGAGGGGACCTTCGGCATGTACGAGCCCTCCGGCGGCAGCGCCCCTGACATAGCTGGCCAGG
GGATCGCCAACCCCATCGCCCAGATCCTCTCCATGGGGATGATGCTCAAGTTCTCCTTCGGCATGGTCGACGCGGCCGAC
GCCATCGACAACGCGGTGGCAACGGTGCTTGACCAGGGCTTCCGGACCCGCGACATCTACCAGCAGAAAGACGGCGAGAA
ACTCGTCAACACCAAGGAGATGGGCGACGCGATCATCGCAGCCCTGTAA

Upstream 100 bases:

>100_bases
CGGGCCCCTTTTCGGATTTCCGGATTTTTGTTGACAACCATTTGTATTCACCAATAGAATTGAGAACTTTTGGAATTTTT
ACTGCAAGAAGGGGGTTCGG

Downstream 100 bases:

>100_bases
CGGCTCAACCAAAATTCATTCCCGAAAAAGGAGTTCTGCCTATGAAAGTCGGAATCGTCGGTTGGCGCGGTATGGTCGGT
TCGGTCCTCCTCCAGCGGAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAQVFKVAVLPGDGIGPEVMAEALRVLDAVEAKYDVKFERTHANVGGAGIDIEGKALPETTVNICKAADAILFGSVGGPK
WESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRV
GFDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLKFSFGMVDAAD
AIDNAVATVLDQGFRTRDIYQQKDGEKLVNTKEMGDAIIAAL

Sequences:

>Translated_362_residues
MAQVFKVAVLPGDGIGPEVMAEALRVLDAVEAKYDVKFERTHANVGGAGIDIEGKALPETTVNICKAADAILFGSVGGPK
WESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRV
GFDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLKFSFGMVDAAD
AIDNAVATVLDQGFRTRDIYQQKDGEKLVNTKEMGDAIIAAL
>Mature_361_residues
AQVFKVAVLPGDGIGPEVMAEALRVLDAVEAKYDVKFERTHANVGGAGIDIEGKALPETTVNICKAADAILFGSVGGPKW
ESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVG
FDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQFD
VILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLKFSFGMVDAADA
IDNAVATVLDQGFRTRDIYQQKDGEKLVNTKEMGDAIIAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=33.8235294117647, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4758582, Length=337, Percent_Identity=29.3768545994065, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=29.1793313069909, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178821, Length=342, Percent_Identity=28.3625730994152, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=29.3929712460064, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=32.7485380116959, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=60.8938547486033, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=34.8571428571429, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=25.8620689655172, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=310, Percent_Identity=33.2258064516129, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=32.1937321937322, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=348, Percent_Identity=29.0229885057471, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=346, Percent_Identity=29.7687861271676, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=44.7154471544715, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=34.4444444444444, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=31.7647058823529, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=32.7433628318584, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=34.0361445783133, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=34.0361445783133, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24661184, Length=369, Percent_Identity=30.8943089430894, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=29.3255131964809, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=29.3255131964809, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=29.3255131964809, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=29.3255131964809, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078639, Length=339, Percent_Identity=29.2035398230088, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281362242, Length=336, Percent_Identity=27.6785714285714, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648872, Length=336, Percent_Identity=27.6785714285714, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38846; Mature: 38715

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVFKVAVLPGDGIGPEVMAEALRVLDAVEAKYDVKFERTHANVGGAGIDIEGKALPET
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEECCCCCCCHH
TVNICKAADAILFGSVGGPKWESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHHHHCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVGFDTMRYSVPEIERITHVAF
HHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSMGMMLKFSFGMVDAADAIDNAVATVLDQGFRTRDIYQQKDGEKLVNTKEMGDAIIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCHHHHHHHHHH
AL
CC
>Mature Secondary Structure 
AQVFKVAVLPGDGIGPEVMAEALRVLDAVEAKYDVKFERTHANVGGAGIDIEGKALPET
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEECCCCCCCHH
TVNICKAADAILFGSVGGPKWESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHHHHCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVGFDTMRYSVPEIERITHVAF
HHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSMGMMLKFSFGMVDAADAIDNAVATVLDQGFRTRDIYQQKDGEKLVNTKEMGDAIIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCHHHHHHHHHH
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA