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The map label for this gene is walK [H]

Identifier: 39996976

GI number: 39996976

Start: 2056980

End: 2057969

Strand: Direct

Name: walK [H]

Synonym: GSU1878

Alternate gene names: 39996976

Gene position: 2056980-2057969 (Clockwise)

Preceding gene: 39996971

Following gene: 39996977

Centisome position: 53.93

GC content: 59.8

Gene sequence:

>990_bases
TTGCAAACCCGGCGACAGGGTGCTACACACAATCCCATGAGATGGCTCAGGAAGCTTGCCAATCCGCTTTTCGCTCTTAT
CGGCATCCAGGTGATATGGGGACTCTTCGTCTTCTACTGGATCTACTGGTTCGTGGGGCGCCACAAGGAGTTCCGGGAAC
TGGCCCTTCGCTACCGGCCCGAGCTGCTCGGCCGCGGTTTCGACTGGATGGTGCTCGTGGAAGGGCTCATCCTGCTGGTG
CTCATCCTGGCCGGGGTCTATGCGCTCTTCATCTTCTGGCGCCGCCAGTCCAACCTCTATCGTGAGCAGAAGGAATTCAT
CTCCCAGGTCACCCACGAGCTCAAGTCGCCGCTGGCATCCATCAAGCTCCACCTTGAGACGATCCGGCTGCGCAAGCCGC
CGCCCGACAAGCTGGAGCGGTTCCTCGACACCATGCTTGAGGACACCGAGCGCCTCGACAACCTCATCAGCAACTTCCTC
ATGGCGGCAAAGCTTGAGCAAAAGCTCCGCACGACCCAGCAAACCGTCGTCGACTTTTCCGCCTTCCTTGTCCGCTCCCT
GGAGCGCCTGGAACAGCATCTGCCCGAGGGAGGAAACCTGACGCTCCGGATAGACGAGGGAATCCGGGCGGCCATCGATG
CGGACGCCATGGAAACGGCACTGCGAAATCTCTTCGAAAACGCAGTCCTTTACAGCCCCGGAGCTCCCGATATCAGGGTT
GCCCTGACCCGCTCCGGCAATGACTGTATCCTCACCTTCAGCGATCAGGGCGTTGGCATCGACCCCCACGACATCAAGAA
GATTTTCAAGATGTTCTACCGGGTCCGCAGACCGGGCGAAACCATCCGGGGAACCGGCCTGGGACTCTGGATCGTTAAAT
CGGTCATCCGGGAGCATGGAGGGCGAGTCGCCGTTTCGAGCAGAGGAAATGGCATGGGAACGACCTTCACCATTACACTC
CCGCTAGCCCGCGGAGGGGATCGTCAATGA

Upstream 100 bases:

>100_bases
GGTCCGACCATGTACACCTCCGTGGTGTCGTTTGTATACGCCCGTTACACCATAGCAGAACCGCCAGGATGCAGGTGTAA
TAGTTTTGTAAAAGGAAACC

Downstream 100 bases:

>100_bases
CCGAGGACAAGCCGCACATTTTGCTCGTTGAAGACGAAATCCACCTGGCTCGGGGGATCTGCTTCAACCTGGAGATGGAA
GGATACCGCGTGAGCCACGT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQTRRQGATHNPMRWLRKLANPLFALIGIQVIWGLFVFYWIYWFVGRHKEFRELALRYRPELLGRGFDWMVLVEGLILLV
LILAGVYALFIFWRRQSNLYREQKEFISQVTHELKSPLASIKLHLETIRLRKPPPDKLERFLDTMLEDTERLDNLISNFL
MAAKLEQKLRTTQQTVVDFSAFLVRSLERLEQHLPEGGNLTLRIDEGIRAAIDADAMETALRNLFENAVLYSPGAPDIRV
ALTRSGNDCILTFSDQGVGIDPHDIKKIFKMFYRVRRPGETIRGTGLGLWIVKSVIREHGGRVAVSSRGNGMGTTFTITL
PLARGGDRQ

Sequences:

>Translated_329_residues
MQTRRQGATHNPMRWLRKLANPLFALIGIQVIWGLFVFYWIYWFVGRHKEFRELALRYRPELLGRGFDWMVLVEGLILLV
LILAGVYALFIFWRRQSNLYREQKEFISQVTHELKSPLASIKLHLETIRLRKPPPDKLERFLDTMLEDTERLDNLISNFL
MAAKLEQKLRTTQQTVVDFSAFLVRSLERLEQHLPEGGNLTLRIDEGIRAAIDADAMETALRNLFENAVLYSPGAPDIRV
ALTRSGNDCILTFSDQGVGIDPHDIKKIFKMFYRVRRPGETIRGTGLGLWIVKSVIREHGGRVAVSSRGNGMGTTFTITL
PLARGGDRQ
>Mature_329_residues
MQTRRQGATHNPMRWLRKLANPLFALIGIQVIWGLFVFYWIYWFVGRHKEFRELALRYRPELLGRGFDWMVLVEGLILLV
LILAGVYALFIFWRRQSNLYREQKEFISQVTHELKSPLASIKLHLETIRLRKPPPDKLERFLDTMLEDTERLDNLISNFL
MAAKLEQKLRTTQQTVVDFSAFLVRSLERLEQHLPEGGNLTLRIDEGIRAAIDADAMETALRNLFENAVLYSPGAPDIRV
ALTRSGNDCILTFSDQGVGIDPHDIKKIFKMFYRVRRPGETIRGTGLGLWIVKSVIREHGGRVAVSSRGNGMGTTFTITL
PLARGGDRQ

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=30.4347826086957, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790861, Length=209, Percent_Identity=31.1004784688995, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=30.2127659574468, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=28.0172413793103, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790436, Length=261, Percent_Identity=27.2030651340996, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=29.0748898678414, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=29.2035398230088, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=25.9109311740891, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790551, Length=310, Percent_Identity=27.0967741935484, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789403, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=26.9911504424779, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=25.8771929824561, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37833; Mature: 37833

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRRQGATHNPMRWLRKLANPLFALIGIQVIWGLFVFYWIYWFVGRHKEFRELALRYRP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCH
ELLGRGFDWMVLVEGLILLVLILAGVYALFIFWRRQSNLYREQKEFISQVTHELKSPLAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLHLETIRLRKPPPDKLERFLDTMLEDTERLDNLISNFLMAAKLEQKLRTTQQTVVDFS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLVRSLERLEQHLPEGGNLTLRIDEGIRAAIDADAMETALRNLFENAVLYSPGAPDIRV
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEE
ALTRSGNDCILTFSDQGVGIDPHDIKKIFKMFYRVRRPGETIRGTGLGLWIVKSVIREHG
EEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GRVAVSSRGNGMGTTFTITLPLARGGDRQ
CEEEEECCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MQTRRQGATHNPMRWLRKLANPLFALIGIQVIWGLFVFYWIYWFVGRHKEFRELALRYRP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCH
ELLGRGFDWMVLVEGLILLVLILAGVYALFIFWRRQSNLYREQKEFISQVTHELKSPLAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLHLETIRLRKPPPDKLERFLDTMLEDTERLDNLISNFLMAAKLEQKLRTTQQTVVDFS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLVRSLERLEQHLPEGGNLTLRIDEGIRAAIDADAMETALRNLFENAVLYSPGAPDIRV
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEE
ALTRSGNDCILTFSDQGVGIDPHDIKKIFKMFYRVRRPGETIRGTGLGLWIVKSVIREHG
EEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GRVAVSSRGNGMGTTFTITLPLARGGDRQ
CEEEEECCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA