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The map label for this gene is nifM [H]

Identifier: 39995469

GI number: 39995469

Start: 392844

End: 393737

Strand: Reverse

Name: nifM [H]

Synonym: GSU0361

Alternate gene names: 39995469

Gene position: 393737-392844 (Counterclockwise)

Preceding gene: 39995471

Following gene: 39995467

Centisome position: 10.32

GC content: 59.96

Gene sequence:

>894_bases
ATGACTTCAAGGACTTTACTGGTGGGCGCGCTCCTCGTGGCATTGGCAGCCGGCGTATCTCGGGCGGAAGAAATCAACCC
CGTGGTGGGGAAGGCGGGCGAGTTCACCCTTCGAGAGGCGGACCTGGATCGGTTCATCGATGCCCTGCCGGCCGAAGCAC
AGCAGCAGCTCCGAAAAAATCCGGAGCAGCGGTCCCTTCTGGCCCGGGAGATCCTGCAGACCCGTGCCATCGCCATGAAA
GCCAGAAAAGACGGTTTCGACCGCAAGCCTGAGGTGAGAGAGCGGCTCGGCTATTTTGTGGACGATTTCCTGGCCCGCGA
ATACCTGACCAAGGTCGTCATTGCCGGCGTGAAGGTGTCCGATGCAGAACTGCGAGCCTATTACCAGGAGCATGCCAAGG
ACTTTGTCATGCCTGAGCAGGCCATAGTCCGGCACATCTTCATCGGGTTCACCGACGCTGCCGACAGCCAAGCCAAGGCA
GCGGCCAAGACAAAGACTGAAGGAGTGCTCGAACGGCTGAAGAAGGGAGAAGACTTTGCCGCCGTGGCGGCGGAGGCATC
CGAGGACATCGAGTCGGCAAAAGAAGGAGGCCTGCTCGGCGCCATCACTCCCGGGCAAACCAATTCCGAGGAATTCGAAA
AGGCGGTGTTCGCCCTCAAGGCTGGCGAGATGAGTGGCTTGGTTGAGAGCCCCTTCGGTTATCACATCGTCAAGGTGGAT
GAACGGAAGGAGAAAAGGGCCCTCCCCTTCGACGAGGTGAAGGATGCCCTGCGCTCCAAACTGCAGGGGGAGGCGGAGCA
GAAAAAACTGAGGGAATTCGTCGACGAGGTCGCTAAACAGACGGGGCTGGAACTGCCCGCCAGCGAGGAGAAGCCGTCAG
GCGAGGGGAAATAG

Upstream 100 bases:

>100_bases
CACGCCCCAAGTGGCGCTGGCTTTTCCCCAAGGCCTGTGCTAATAGGTCGGATAAACTCATGAATACGCATTCCACACCA
TCATGACAGAGGGAGGACAC

Downstream 100 bases:

>100_bases
CCGCGGGCATCGCGAATGGGCAAGGCATCGAATTGGGCCTCGCCAGCAACCAATACACACGGCGCGTTGCCTGAGCAACG
CGCCGTTTTTCCTTTCCGGG

Product: peptidyl-prolyl cis-trans isomerase domain-containing protein

Products: peptidylproline (omega=0) [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTSRTLLVGALLVALAAGVSRAEEINPVVGKAGEFTLREADLDRFIDALPAEAQQQLRKNPEQRSLLAREILQTRAIAMK
ARKDGFDRKPEVRERLGYFVDDFLAREYLTKVVIAGVKVSDAELRAYYQEHAKDFVMPEQAIVRHIFIGFTDAADSQAKA
AAKTKTEGVLERLKKGEDFAAVAAEASEDIESAKEGGLLGAITPGQTNSEEFEKAVFALKAGEMSGLVESPFGYHIVKVD
ERKEKRALPFDEVKDALRSKLQGEAEQKKLREFVDEVAKQTGLELPASEEKPSGEGK

Sequences:

>Translated_297_residues
MTSRTLLVGALLVALAAGVSRAEEINPVVGKAGEFTLREADLDRFIDALPAEAQQQLRKNPEQRSLLAREILQTRAIAMK
ARKDGFDRKPEVRERLGYFVDDFLAREYLTKVVIAGVKVSDAELRAYYQEHAKDFVMPEQAIVRHIFIGFTDAADSQAKA
AAKTKTEGVLERLKKGEDFAAVAAEASEDIESAKEGGLLGAITPGQTNSEEFEKAVFALKAGEMSGLVESPFGYHIVKVD
ERKEKRALPFDEVKDALRSKLQGEAEQKKLREFVDEVAKQTGLELPASEEKPSGEGK
>Mature_296_residues
TSRTLLVGALLVALAAGVSRAEEINPVVGKAGEFTLREADLDRFIDALPAEAQQQLRKNPEQRSLLAREILQTRAIAMKA
RKDGFDRKPEVRERLGYFVDDFLAREYLTKVVIAGVKVSDAELRAYYQEHAKDFVMPEQAIVRHIFIGFTDAADSQAKAA
AKTKTEGVLERLKKGEDFAAVAAEASEDIESAKEGGLLGAITPGQTNSEEFEKAVFALKAGEMSGLVESPFGYHIVKVDE
RKEKRALPFDEVKDALRSKLQGEAEQKKLREFVDEVAKQTGLELPASEEKPSGEGK

Specific function: Required for the activation and stabilization of the iron-component (nifH) of nitrogenase. Probable PPIase [H]

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Type II Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

Organism=Escherichia coli, GI1786645, Length=150, Percent_Identity=34.6666666666667, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014282
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: 5.2.1.8 [C]

Molecular weight: Translated: 32693; Mature: 32562

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01096 PPIC_PPIASE_1 ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTLLVGALLVALAAGVSRAEEINPVVGKAGEFTLREADLDRFIDALPAEAQQQLRKN
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
PEQRSLLAREILQTRAIAMKARKDGFDRKPEVRERLGYFVDDFLAREYLTKVVIAGVKVS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DAELRAYYQEHAKDFVMPEQAIVRHIFIGFTDAADSQAKAAAKTKTEGVLERLKKGEDFA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
AVAAEASEDIESAKEGGLLGAITPGQTNSEEFEKAVFALKAGEMSGLVESPFGYHIVKVD
HHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
ERKEKRALPFDEVKDALRSKLQGEAEQKKLREFVDEVAKQTGLELPASEEKPSGEGK
CHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSRTLLVGALLVALAAGVSRAEEINPVVGKAGEFTLREADLDRFIDALPAEAQQQLRKN
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
PEQRSLLAREILQTRAIAMKARKDGFDRKPEVRERLGYFVDDFLAREYLTKVVIAGVKVS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DAELRAYYQEHAKDFVMPEQAIVRHIFIGFTDAADSQAKAAAKTKTEGVLERLKKGEDFA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
AVAAEASEDIESAKEGGLLGAITPGQTNSEEFEKAVFALKAGEMSGLVESPFGYHIVKVD
HHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
ERKEKRALPFDEVKDALRSKLQGEAEQKKLREFVDEVAKQTGLELPASEEKPSGEGK
CHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Peptidoproline (omega=180) [C]

Specific reaction: Peptidoproline (omega=180) = peptidylproline (omega=0) [C]

General reaction: Rotation (Single bond) [C]

Inhibitor: cyclosporins [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1885524 [H]