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The map label for this gene is hyfR [H]

Identifier: 39995467

GI number: 39995467

Start: 389808

End: 391184

Strand: Reverse

Name: hyfR [H]

Synonym: GSU0359

Alternate gene names: 39995467

Gene position: 391184-389808 (Counterclockwise)

Preceding gene: 39995469

Following gene: 39995466

Centisome position: 10.26

GC content: 60.42

Gene sequence:

>1377_bases
GTGCGCACTGAACCACAGCCGCTTCATTTGCCCGTTTTTCGGGCAATTCTCGCCAGCATGGGAGAGGGGATCATCTTCGC
CGACGCAGACGACAGAATCCGCTATATCAATGCGGCCGCCGAGGATATCCGCGGAATCATCGCCGGCAACTATCTGGGGC
GGAGCATCGTCTCCATTCACTCACCCGCGTCCGAAGGGCGCATCCGTCGGCTCATCGCCGGGCTCAAGGGGGGTGACATC
CCTTTTTCCACCCGAGTGATCCACGTAAGGGAAAAGATCTTCGAAAACAGCTACTACCCCATCCGAAACGAGAACGGGAT
CTACGAGGGGACTCTCCTGATCTCGCGGGACATCACCGAGAAAGAGCTCCTCAAGGAGGAAAACAACTCCTTGAGGGAGC
AGGTACGCGCCGACTTCGCCTGCGGCGGAATGATCGGAAAAAGTCATGCCATGGAACCGGTGTTCCACATGATCAGGGCT
ACGGCAGCCCTTGATTCCACCATCCTCATCTCCGGCGAAAGCGGGACAGGCAAGGAACTGGTGGCCTGTGCCATTCACCA
GATGAGCCCGCGCCACAAGAATCCCTTCGTCAAGGTAAACTGCGCCGCCCTCCCGGAAACGCTTCTGGAATCGGAACTTT
TCGGCCACGAGCGGGGAGCATTCACGGGGGCGGTGCGGGAACGCAAGGGGAAATTCGAACAGGCCCACCTGGGAACCATT
TTCCTCGACGAAATCGGCGAGATGCCCCTGGCCGCCCAAGCAAAGCTGTTGCGCGTAATCCAGGGAAAGACCCTGGAGCG
GATCGGAGGCAACCGCGAGATCGCCGTTGATGTGCGGATCATCGCTGCCACCAACAAAGATCTGCGCAGGGAAGTCGAGC
GCGGGGCCTTTCGGGAAGACCTTTTTTACCGCCTCAACGTCATTCCCATCCATCTGCCGCCGCTCCGGGAGCGCCGTGAA
GACATTCTCCCCTTGGCCGAGCACTTTCTGTCCGTCTACGCAGAGCGGATGCGCAAACCGGTCCATTGCATCGCCCCAGA
CGCCCGCAAGGCACTCCTGGACTATGACTATCCGGGGAACGTCCGGGAGTTGGAGAATGCCATTGAGCGAGGCGTGGCCC
TGTCGATGGGCGAGACCATCACCCTGGCCGATCTTGCCCCCTACTTCGTAACGGGAGCATCTGCGGCGCCGGCTGGTGCC
GCTCTCCCCAAGGAGCCACCGACGGCAACCCTCTCCCGGGCGGTATCCGTCGCCGAACAGGAGGCCATCCGCGAAGCGCT
GCAGACAACCGGCAACCGCAAGGGGGTGGCGGCAAAACTCCTCGGCATCTCCCGCAAGACTCTTTGGGAAAAACTTAAAC
AGATGGAGCGCACGTAA

Upstream 100 bases:

>100_bases
ACAGGCGGCTGCCGCGACTTTCTCGTCCCTAATCACGGGAAAATAGTGGAGTCGAAATCGTTGCCGTGTTACGATCAAGT
AACATTCGGGAGGACTTACC

Downstream 100 bases:

>100_bases
CGCCCACCACACATACGTTACCTCAAAGTAACACTTCGCCACATTCTAAAGCGCAAACCGTCGCCGCCGCCATCTCCTAC
CACCAACAATTACGGCTAAT

Product: sensory box protein/sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRTEPQPLHLPVFRAILASMGEGIIFADADDRIRYINAAAEDIRGIIAGNYLGRSIVSIHSPASEGRIRRLIAGLKGGDI
PFSTRVIHVREKIFENSYYPIRNENGIYEGTLLISRDITEKELLKEENNSLREQVRADFACGGMIGKSHAMEPVFHMIRA
TAALDSTILISGESGTGKELVACAIHQMSPRHKNPFVKVNCAALPETLLESELFGHERGAFTGAVRERKGKFEQAHLGTI
FLDEIGEMPLAAQAKLLRVIQGKTLERIGGNREIAVDVRIIAATNKDLRREVERGAFREDLFYRLNVIPIHLPPLRERRE
DILPLAEHFLSVYAERMRKPVHCIAPDARKALLDYDYPGNVRELENAIERGVALSMGETITLADLAPYFVTGASAAPAGA
ALPKEPPTATLSRAVSVAEQEAIREALQTTGNRKGVAAKLLGISRKTLWEKLKQMERT

Sequences:

>Translated_458_residues
MRTEPQPLHLPVFRAILASMGEGIIFADADDRIRYINAAAEDIRGIIAGNYLGRSIVSIHSPASEGRIRRLIAGLKGGDI
PFSTRVIHVREKIFENSYYPIRNENGIYEGTLLISRDITEKELLKEENNSLREQVRADFACGGMIGKSHAMEPVFHMIRA
TAALDSTILISGESGTGKELVACAIHQMSPRHKNPFVKVNCAALPETLLESELFGHERGAFTGAVRERKGKFEQAHLGTI
FLDEIGEMPLAAQAKLLRVIQGKTLERIGGNREIAVDVRIIAATNKDLRREVERGAFREDLFYRLNVIPIHLPPLRERRE
DILPLAEHFLSVYAERMRKPVHCIAPDARKALLDYDYPGNVRELENAIERGVALSMGETITLADLAPYFVTGASAAPAGA
ALPKEPPTATLSRAVSVAEQEAIREALQTTGNRKGVAAKLLGISRKTLWEKLKQMERT
>Mature_458_residues
MRTEPQPLHLPVFRAILASMGEGIIFADADDRIRYINAAAEDIRGIIAGNYLGRSIVSIHSPASEGRIRRLIAGLKGGDI
PFSTRVIHVREKIFENSYYPIRNENGIYEGTLLISRDITEKELLKEENNSLREQVRADFACGGMIGKSHAMEPVFHMIRA
TAALDSTILISGESGTGKELVACAIHQMSPRHKNPFVKVNCAALPETLLESELFGHERGAFTGAVRERKGKFEQAHLGTI
FLDEIGEMPLAAQAKLLRVIQGKTLERIGGNREIAVDVRIIAATNKDLRREVERGAFREDLFYRLNVIPIHLPPLRERRE
DILPLAEHFLSVYAERMRKPVHCIAPDARKALLDYDYPGNVRELENAIERGVALSMGETITLADLAPYFVTGASAAPAGA
ALPKEPPTATLSRAVSVAEQEAIREALQTTGNRKGVAAKLLGISRKTLWEKLKQMERT

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=349, Percent_Identity=46.1318051575931, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=50.9615384615385, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=47.8753541076487, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=47.3529411764706, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1790299, Length=364, Percent_Identity=43.1318681318681, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789233, Length=457, Percent_Identity=35.6673960612691, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082152, Length=302, Percent_Identity=48.3443708609272, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1786524, Length=351, Percent_Identity=43.3048433048433, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1788905, Length=314, Percent_Identity=45.2229299363057, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=40.5660377358491, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=370, Percent_Identity=35.6756756756757, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081858, Length=457, Percent_Identity=30.8533916849015, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=39.2857142857143, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50703; Mature: 50703

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEPQPLHLPVFRAILASMGEGIIFADADDRIRYINAAAEDIRGIIAGNYLGRSIVSIH
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEC
SPASEGRIRRLIAGLKGGDIPFSTRVIHVREKIFENSYYPIRNENGIYEGTLLISRDITE
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCH
KELLKEENNSLREQVRADFACGGMIGKSHAMEPVFHMIRATAALDSTILISGESGTGKEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VACAIHQMSPRHKNPFVKVNCAALPETLLESELFGHERGAFTGAVRERKGKFEQAHLGTI
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCEE
FLDEIGEMPLAAQAKLLRVIQGKTLERIGGNREIAVDVRIIAATNKDLRREVERGAFRED
EHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHCCHHHH
LFYRLNVIPIHLPPLRERREDILPLAEHFLSVYAERMRKPVHCIAPDARKALLDYDYPGN
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCC
VRELENAIERGVALSMGETITLADLAPYFVTGASAAPAGAALPKEPPTATLSRAVSVAEQ
HHHHHHHHHCCCEEECCCEEEHHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EAIREALQTTGNRKGVAAKLLGISRKTLWEKLKQMERT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTEPQPLHLPVFRAILASMGEGIIFADADDRIRYINAAAEDIRGIIAGNYLGRSIVSIH
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEC
SPASEGRIRRLIAGLKGGDIPFSTRVIHVREKIFENSYYPIRNENGIYEGTLLISRDITE
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCH
KELLKEENNSLREQVRADFACGGMIGKSHAMEPVFHMIRATAALDSTILISGESGTGKEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VACAIHQMSPRHKNPFVKVNCAALPETLLESELFGHERGAFTGAVRERKGKFEQAHLGTI
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCEE
FLDEIGEMPLAAQAKLLRVIQGKTLERIGGNREIAVDVRIIAATNKDLRREVERGAFRED
EHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHCCHHHH
LFYRLNVIPIHLPPLRERREDILPLAEHFLSVYAERMRKPVHCIAPDARKALLDYDYPGN
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCC
VRELENAIERGVALSMGETITLADLAPYFVTGASAAPAGAALPKEPPTATLSRAVSVAEQ
HHHHHHHHHCCCEEECCCEEEHHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EAIREALQTTGNRKGVAAKLLGISRKTLWEKLKQMERT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]