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The map label for this gene is radA [H]

Identifier: 39995394

GI number: 39995394

Start: 313908

End: 315260

Strand: Reverse

Name: radA [H]

Synonym: GSU0285

Alternate gene names: 39995394

Gene position: 315260-313908 (Counterclockwise)

Preceding gene: 39995397

Following gene: 39995393

Centisome position: 8.27

GC content: 64.52

Gene sequence:

>1353_bases
GTGAAAACCGTAACCCGTTATGCCTGCCAGAAATGCGGCTACCAATCGGCCAAATGGCTTGGGAAATGCCCCGACTGCGC
CGCCTGGAACAGTTTTGTCGAGGAAGTCACCGGCGGCGGACGAAAGGGTGCCGCCTCCCTGCCCGCAGCCACCCGCCCGG
TCCCCATCTGTGATGTGGCCAGCGAGGCGGAAACACGCCTGTCGTGCGGCATCTCCGAATTCGACCGGGTACTGGGCGGT
GGTCTCGTCTCCGGCTCCCTGGTGCTCATCGGCGGCGACCCCGGCATCGGCAAATCCACCATTCTTTTGCAAGTCATGGA
CCACCTTGCGCGGACCGCGGGACCGGTTCTCTACGTATCGGGAGAGGAATCGGCGCGACAAACAAAAATGCGCGGCGAAC
GCCTGGGCATCGGAGCACGTGAACTGCTCGTCCTCGCCGAGACCTCCCTCGAACTCATCCTGTCCCAAGTTACCGCTCTC
GCCCCCCGGGCCCTTGTGATCGACTCCATCCAGACCGTTTTCAGCGCGAACCTCGAATCGGCGCCCGGCAGCGTCAGCCA
GGTGCGCGAGTGCGCCGGCAGGCTCATGGAGCTGGCCAAGCGCAACGGCATCCCCACCCTCCTTGTGGGACACGTGACCA
AGGACGGCTCCCTGGCGGGCCCACGGGTGCTGGAGCACATGGTCGACACGGTGCTCTACTTCGAAGGGGACCCGGGGCAT
GCCTTCAGGATTCTCAGGGCCGTCAAGAACCGCTTCGGCTCAACCAATGAAATCGGTGTATTCGAGATGAAAGAGGGCGG
CCTCAGCGAAGTGAGAAACCCCTCGGAGCTCTTCCTGGCCGAACGGCCGCTCGGCGTTTCGGGGTCCGCCGTCGTCACCA
CCATCGAGGGGAGCCGACCGCTGCTGGTGGAACTGCAGGCACTGGTCACCCCCTCATCCCTGGGCGTTCCACGCCGCACC
ACTCTCGGGGTTGACCATAACCGCCTCGCGCTCCTGGTAGCCGTGCTCGACAAAAAAGTCGGCCTCCACCTGTCGGGGCA
GGACATCTTCCTGAACGCCGCCGGCGGCGCACGGCTCAGCGAGCCGGCCATCGACCTGGGCATGGTGGCGGCCCTCGCAT
CAAGCCACTTGGACAAAATAATCGATCCCCACACGGTCATTGTCGGCGAGGTGGGCCTTGCGGGGGAAGTCCGCGCCATT
ACCCAGGCGGAGACACGGGTCCGCGAGGCCGCCAAGCTGGGCTTCACACGGGCGGTTATCCCCGCCGGCAGCGTCCGACA
GGTCGGCGATGTTCCCGGCATGAACGTTGTGGGAGTGAAATCCCTGGATGAAGCGCTGGAACAGCTCTTCTGA

Upstream 100 bases:

>100_bases
ACAGGTGTCAAACTCACAGACTTCACCTAATGCCGACGGTCGATTGACAGCCGAGTGTCAAGGCGGTAGTATCCGCTCAC
TCCCGAGACGAGGACACCCC

Downstream 100 bases:

>100_bases
CTGATCCGACGCGCTTTTTTCCTTTACTTGATTAGCCGTTTCTCTTACTATTTACAGCAGTTATACATAAAGGAGATTCG
GTGAATGGAAAGCGAGAAGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKTVTRYACQKCGYQSAKWLGKCPDCAAWNSFVEEVTGGGRKGAASLPAATRPVPICDVASEAETRLSCGISEFDRVLGG
GLVSGSLVLIGGDPGIGKSTILLQVMDHLARTAGPVLYVSGEESARQTKMRGERLGIGARELLVLAETSLELILSQVTAL
APRALVIDSIQTVFSANLESAPGSVSQVRECAGRLMELAKRNGIPTLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGDPGH
AFRILRAVKNRFGSTNEIGVFEMKEGGLSEVRNPSELFLAERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRT
TLGVDHNRLALLVAVLDKKVGLHLSGQDIFLNAAGGARLSEPAIDLGMVAALASSHLDKIIDPHTVIVGEVGLAGEVRAI
TQAETRVREAAKLGFTRAVIPAGSVRQVGDVPGMNVVGVKSLDEALEQLF

Sequences:

>Translated_450_residues
MKTVTRYACQKCGYQSAKWLGKCPDCAAWNSFVEEVTGGGRKGAASLPAATRPVPICDVASEAETRLSCGISEFDRVLGG
GLVSGSLVLIGGDPGIGKSTILLQVMDHLARTAGPVLYVSGEESARQTKMRGERLGIGARELLVLAETSLELILSQVTAL
APRALVIDSIQTVFSANLESAPGSVSQVRECAGRLMELAKRNGIPTLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGDPGH
AFRILRAVKNRFGSTNEIGVFEMKEGGLSEVRNPSELFLAERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRT
TLGVDHNRLALLVAVLDKKVGLHLSGQDIFLNAAGGARLSEPAIDLGMVAALASSHLDKIIDPHTVIVGEVGLAGEVRAI
TQAETRVREAAKLGFTRAVIPAGSVRQVGDVPGMNVVGVKSLDEALEQLF
>Mature_450_residues
MKTVTRYACQKCGYQSAKWLGKCPDCAAWNSFVEEVTGGGRKGAASLPAATRPVPICDVASEAETRLSCGISEFDRVLGG
GLVSGSLVLIGGDPGIGKSTILLQVMDHLARTAGPVLYVSGEESARQTKMRGERLGIGARELLVLAETSLELILSQVTAL
APRALVIDSIQTVFSANLESAPGSVSQVRECAGRLMELAKRNGIPTLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGDPGH
AFRILRAVKNRFGSTNEIGVFEMKEGGLSEVRNPSELFLAERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRT
TLGVDHNRLALLVAVLDKKVGLHLSGQDIFLNAAGGARLSEPAIDLGMVAALASSHLDKIIDPHTVIVGEVGLAGEVRAI
TQAETRVREAAKLGFTRAVIPAGSVRQVGDVPGMNVVGVKSLDEALEQLF

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=448, Percent_Identity=46.4285714285714, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47330; Mature: 47330

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTRYACQKCGYQSAKWLGKCPDCAAWNSFVEEVTGGGRKGAASLPAATRPVPICDVA
CCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCC
SEAETRLSCGISEFDRVLGGGLVSGSLVLIGGDPGIGKSTILLQVMDHLARTAGPVLYVS
HHHHHHHHCCHHHHHHHHCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEC
GEESARQTKMRGERLGIGARELLVLAETSLELILSQVTALAPRALVIDSIQTVFSANLES
CCHHHHHHHHCHHHCCCCHHHHEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
APGSVSQVRECAGRLMELAKRNGIPTLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGDPGH
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCH
AFRILRAVKNRFGSTNEIGVFEMKEGGLSEVRNPSELFLAERPLGVSGSAVVTTIEGSRP
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCHHHHEECCCCCCCCCEEEEEECCCCC
LLVELQALVTPSSLGVPRRTTLGVDHNRLALLVAVLDKKVGLHLSGQDIFLNAAGGARLS
EEEEHHHHCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCC
EPAIDLGMVAALASSHLDKIIDPHTVIVGEVGLAGEVRAITQAETRVREAAKLGFTRAVI
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEC
PAGSVRQVGDVPGMNVVGVKSLDEALEQLF
CCCCCHHHCCCCCCCEECHHHHHHHHHHHC
>Mature Secondary Structure
MKTVTRYACQKCGYQSAKWLGKCPDCAAWNSFVEEVTGGGRKGAASLPAATRPVPICDVA
CCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCC
SEAETRLSCGISEFDRVLGGGLVSGSLVLIGGDPGIGKSTILLQVMDHLARTAGPVLYVS
HHHHHHHHCCHHHHHHHHCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEC
GEESARQTKMRGERLGIGARELLVLAETSLELILSQVTALAPRALVIDSIQTVFSANLES
CCHHHHHHHHCHHHCCCCHHHHEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
APGSVSQVRECAGRLMELAKRNGIPTLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGDPGH
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCH
AFRILRAVKNRFGSTNEIGVFEMKEGGLSEVRNPSELFLAERPLGVSGSAVVTTIEGSRP
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCHHHHEECCCCCCCCCEEEEEECCCCC
LLVELQALVTPSSLGVPRRTTLGVDHNRLALLVAVLDKKVGLHLSGQDIFLNAAGGARLS
EEEEHHHHCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCC
EPAIDLGMVAALASSHLDKIIDPHTVIVGEVGLAGEVRAITQAETRVREAAKLGFTRAVI
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEC
PAGSVRQVGDVPGMNVVGVKSLDEALEQLF
CCCCCHHHCCCCCCCEECHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]