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The map label for this gene is brnQ [H]

Identifier: 38234308

GI number: 38234308

Start: 1779545

End: 1780849

Strand: Direct

Name: brnQ [H]

Synonym: DIP1737

Alternate gene names: 38234308

Gene position: 1779545-1780849 (Clockwise)

Preceding gene: 38234307

Following gene: 38234309

Centisome position: 71.51

GC content: 55.48

Gene sequence:

>1305_bases
ATGGCCTCTCCCACCTCTGAGAAGAAGCTGTCCGGTGGCGCAATCATCGCCACCGCACTCATGCTGTTCTCCATGTTCTT
TGGCGCCGGAAACCTGATTTTCCCACCAGTGCTTGGCGCTAACTCTGGAGAAAACTTCACCCCCGCTATTATCGGATTCC
TCCTCGGCGGTGTCGCGTTGCCAGCAATCACCATTGTTGCCATGACGCTGTCCGGAAACGACATCCGCGATCTCGGATCC
CGCGCCGGCTCATGGTTCAATCTCTCTTTTGCAATCATCGCATACCTGTCCATCGGTGCGTTCTACGCTATCCCACGTAC
CGGCGCCGTCAGTTACTCCACAGTCATCCAACCAGTGATGTCGGAGCCATCCACAGCAGCATCAGTAGGATTCAACGCTC
TGTTCTTCGCCGTGGCGTTACTGTTGTCGCTTAACCCAACGGGAATCGTCGATAAGCTGGGCAAAATCCTTACCCCAGCA
CTGCTCATCCTGTTGGTCATCTTGGTCACCTTGGCAGTATTCAAACTCAATGGCACCGCAGCACCTGCCACCGATTCCTA
TCGCGAGGCTCCGCTCACCAGCGGCCTGCTCGAAGGCTATATGACCATGGACTCCATTGCAGCACTGGTCTTCGGCATCT
TGGTGATCTCGTCTCTGCGCTACCAAGGCGGCAATAACGAGCGCCAAGTCATTGGTGCAGCCGTCAAGGCATCACTGATC
GCCGCAGGTCTGCTGGGCATTATCTACCTCGGCTTGGGCTACATGGGCCACATCATTCCGAACGGTCAAGGTTTTGCAGA
TGGCGCGGCATTACTTTCCTCAGCATCACAGCAGACCATGGGTACCCCAGGCCAAGTCATTTTCGGCCTGATCGTCTTAC
TCGCATGCATGACCACAGCAGTCGGCTTGCTGGCGTCGACAAGCGAATTCTTCAACCGCCTCATCCCAGGCATTTCCTAC
AAGGGCTGGCTGTTCATCTTCGCACTCATATCCTTCGCGGTCGGCAGCTTGGGCCTAGCCAAGGTCCTCGCAGTGGCAGC
GCCTGTCATCACCTTCATCTACCCCATCGCGATCACCCTCGTCGCCATCACAGTGATCGAAATGCTTGTTAAGAACCTGA
ACCTGTTCTGGGGCTTTCGCCTTCCTGCATGGACTGTTACGCTGTGGTCTGCAATTACAGTTATCGCAAGCGATACCCTT
GCATGGGCCCCATTCCATGACATCAGCCTCTCGTGGATCTGGCCCGCTTTGATCGGCTTCGTTCTCGGTCTGAGCATTGA
TAAGGCCCTCGCTTACAAGAGTTAG

Upstream 100 bases:

>100_bases
CCGTGTCCCATCACACATAACCCCCGAATCTCACCTTATGGGAACTTAGTGTTATATTGTGGGAAAGCTTTAACCCGTAT
TTGAAAGTTTGTGAATAACC

Downstream 100 bases:

>100_bases
GATCCTCCCATGAAGCTGTCGCTCATCGACTTCGCCACAATCTACCACGGCGAGCGCCCACGCGACAGCTTCCAACGCTC
CGTCGCCCTCGCACAGCAAG

Product: branched-chain amino acid ABC transport system membrane protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MASPTSEKKLSGGAIIATALMLFSMFFGAGNLIFPPVLGANSGENFTPAIIGFLLGGVALPAITIVAMTLSGNDIRDLGS
RAGSWFNLSFAIIAYLSIGAFYAIPRTGAVSYSTVIQPVMSEPSTAASVGFNALFFAVALLLSLNPTGIVDKLGKILTPA
LLILLVILVTLAVFKLNGTAAPATDSYREAPLTSGLLEGYMTMDSIAALVFGILVISSLRYQGGNNERQVIGAAVKASLI
AAGLLGIIYLGLGYMGHIIPNGQGFADGAALLSSASQQTMGTPGQVIFGLIVLLACMTTAVGLLASTSEFFNRLIPGISY
KGWLFIFALISFAVGSLGLAKVLAVAAPVITFIYPIAITLVAITVIEMLVKNLNLFWGFRLPAWTVTLWSAITVIASDTL
AWAPFHDISLSWIWPALIGFVLGLSIDKALAYKS

Sequences:

>Translated_434_residues
MASPTSEKKLSGGAIIATALMLFSMFFGAGNLIFPPVLGANSGENFTPAIIGFLLGGVALPAITIVAMTLSGNDIRDLGS
RAGSWFNLSFAIIAYLSIGAFYAIPRTGAVSYSTVIQPVMSEPSTAASVGFNALFFAVALLLSLNPTGIVDKLGKILTPA
LLILLVILVTLAVFKLNGTAAPATDSYREAPLTSGLLEGYMTMDSIAALVFGILVISSLRYQGGNNERQVIGAAVKASLI
AAGLLGIIYLGLGYMGHIIPNGQGFADGAALLSSASQQTMGTPGQVIFGLIVLLACMTTAVGLLASTSEFFNRLIPGISY
KGWLFIFALISFAVGSLGLAKVLAVAAPVITFIYPIAITLVAITVIEMLVKNLNLFWGFRLPAWTVTLWSAITVIASDTL
AWAPFHDISLSWIWPALIGFVLGLSIDKALAYKS
>Mature_433_residues
ASPTSEKKLSGGAIIATALMLFSMFFGAGNLIFPPVLGANSGENFTPAIIGFLLGGVALPAITIVAMTLSGNDIRDLGSR
AGSWFNLSFAIIAYLSIGAFYAIPRTGAVSYSTVIQPVMSEPSTAASVGFNALFFAVALLLSLNPTGIVDKLGKILTPAL
LILLVILVTLAVFKLNGTAAPATDSYREAPLTSGLLEGYMTMDSIAALVFGILVISSLRYQGGNNERQVIGAAVKASLIA
AGLLGIIYLGLGYMGHIIPNGQGFADGAALLSSASQQTMGTPGQVIFGLIVLLACMTTAVGLLASTSEFFNRLIPGISYK
GWLFIFALISFAVGSLGLAKVLAVAAPVITFIYPIAITLVAITVIEMLVKNLNLFWGFRLPAWTVTLWSAITVIASDTLA
WAPFHDISLSWIWPALIGFVLGLSIDKALAYKS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=431, Percent_Identity=32.9466357308585, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45354; Mature: 45223

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPTSEKKLSGGAIIATALMLFSMFFGAGNLIFPPVLGANSGENFTPAIIGFLLGGVAL
CCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
PAITIVAMTLSGNDIRDLGSRAGSWFNLSFAIIAYLSIGAFYAIPRTGAVSYSTVIQPVM
HHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SEPSTAASVGFNALFFAVALLLSLNPTGIVDKLGKILTPALLILLVILVTLAVFKLNGTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APATDSYREAPLTSGLLEGYMTMDSIAALVFGILVISSLRYQGGNNERQVIGAAVKASLI
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAGLLGIIYLGLGYMGHIIPNGQGFADGAALLSSASQQTMGTPGQVIFGLIVLLACMTTA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VGLLASTSEFFNRLIPGISYKGWLFIFALISFAVGSLGLAKVLAVAAPVITFIYPIAITL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAITVIEMLVKNLNLFWGFRLPAWTVTLWSAITVIASDTLAWAPFHDISLSWIWPALIGF
HHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHCCCCEECCCHHCHHHHHHHHHHHH
VLGLSIDKALAYKS
HHHHHHHHHHHCCC
>Mature Secondary Structure 
ASPTSEKKLSGGAIIATALMLFSMFFGAGNLIFPPVLGANSGENFTPAIIGFLLGGVAL
CCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
PAITIVAMTLSGNDIRDLGSRAGSWFNLSFAIIAYLSIGAFYAIPRTGAVSYSTVIQPVM
HHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SEPSTAASVGFNALFFAVALLLSLNPTGIVDKLGKILTPALLILLVILVTLAVFKLNGTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APATDSYREAPLTSGLLEGYMTMDSIAALVFGILVISSLRYQGGNNERQVIGAAVKASLI
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAGLLGIIYLGLGYMGHIIPNGQGFADGAALLSSASQQTMGTPGQVIFGLIVLLACMTTA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VGLLASTSEFFNRLIPGISYKGWLFIFALISFAVGSLGLAKVLAVAAPVITFIYPIAITL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAITVIEMLVKNLNLFWGFRLPAWTVTLWSAITVIASDTLAWAPFHDISLSWIWPALIGF
HHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHCCCCEECCCHHCHHHHHHHHHHHH
VLGLSIDKALAYKS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]