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The map label for this gene is lipA [H]

Identifier: 38234216

GI number: 38234216

Start: 1675956

End: 1677044

Strand: Direct

Name: lipA [H]

Synonym: DIP1641

Alternate gene names: 38234216

Gene position: 1675956-1677044 (Clockwise)

Preceding gene: 38234215

Following gene: 38234217

Centisome position: 67.34

GC content: 53.44

Gene sequence:

>1089_bases
GTGTCGACGACCACAGCAATCACGAGTACTCTGCAAAGGGTGACTAAAGCACCCGAAGGACGCAGAATGCTCAGAGTTGA
GGCGCGCAACTCGCAGACCCCCATCGAGAGCAAACCACGTTGGATCCGCACCGCTGTTAAAACCGGCCCTGAATATCAGG
ACATGAAAAAGAAAGTGTCGGGTGCAAGCCTGCACACCGTATGCCAAGAAGCCGGCTGCCCTAATATCCACGAATGCTGG
GAGTCGCGTGAGGCTACCTTCTTGATTGGTGGCGCTAACTGCTCGCGCCGTTGCGATTTCTGCCAGATTAATTCTGCTAA
GCCCGAACCACTCGATCGCGACGAGCCACGTCGCGTTGCAGAATCAGTACGCGAAATGCAGCTGAATTACTCGACCATCA
CTGGTGTGACCCGTGACGATCTTGAAGATGAAGGCGCTTGGCTATATGCAGAAGTTGTTCGCAAGATTCATGAGCTCAAT
CCCCACACCGGCGTAGAAAACCTCACCCCTGATTTCTCCGGCAAGCCTGACTTGTTGCAAGAGGTTTTCGAGGCTCGCCC
CGAAGTTTTTGCTCACAACCTCGAAACGGTTCCTCGCATCTTCAAGCGGATCCGCCCCGCTTTCCGTTACGAGCGCTCCT
TAGACGTGATCCGCCAAGCCCGCGATTTTGGATTGGTTACCAAATCCAACCTGATCTTGGGCATGGGAGAAACCGTCGAC
GAAATCCGTGATGCCTTGGTTGACCTGCACTCGGCAGGTTGTGACATCATCACGATTACCCAATATCTTCGTCCCGGCCC
GATGTACCATCCCATCGATCGTTGGGTAAAACCTGAGGAATTTATCGATCACGCTGATTTTGCGCGTGAACTTGGATTTG
GTGCTGTTATGTCGGGGCCATTGGTACGTTCTTCTTATCGTGCCGGAAAGCTTTATGCCGAGGCTCTCGCGGCTCGCGGT
GAGTCCTTGCCAGAAAACTTGGCTCATTTGGCCACGACTGCCGATGGCAGTACTGCGCAAGAAGCAAATACCTTGTTGGA
AAAGTACGGTCCTTCCCAAGACACCCCTGTAGTGTCGTCGAAGGCTTAA

Upstream 100 bases:

>100_bases
AGCTTTGCTTCGGCTCCGGACCCTACGAAGATTCCACGACGACCCTAATTGCAGATACGACAACAAATTAAGGGGTGCTT
CTTGCTGGTTGTTTACGTTC

Downstream 100 bases:

>100_bases
GACTGGGGCGTAGCCGGTGGGTTATTTAATGAATCCTGTCTTGCCCCGCTAAGGTGGTATGCATGGCTGACGTGAACAAG
CAGGCCGCAAAGGCCGCAAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSTTTAITSTLQRVTKAPEGRRMLRVEARNSQTPIESKPRWIRTAVKTGPEYQDMKKKVSGASLHTVCQEAGCPNIHECW
ESREATFLIGGANCSRRCDFCQINSAKPEPLDRDEPRRVAESVREMQLNYSTITGVTRDDLEDEGAWLYAEVVRKIHELN
PHTGVENLTPDFSGKPDLLQEVFEARPEVFAHNLETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETVD
EIRDALVDLHSAGCDIITITQYLRPGPMYHPIDRWVKPEEFIDHADFARELGFGAVMSGPLVRSSYRAGKLYAEALAARG
ESLPENLAHLATTADGSTAQEANTLLEKYGPSQDTPVVSSKA

Sequences:

>Translated_362_residues
MSTTTAITSTLQRVTKAPEGRRMLRVEARNSQTPIESKPRWIRTAVKTGPEYQDMKKKVSGASLHTVCQEAGCPNIHECW
ESREATFLIGGANCSRRCDFCQINSAKPEPLDRDEPRRVAESVREMQLNYSTITGVTRDDLEDEGAWLYAEVVRKIHELN
PHTGVENLTPDFSGKPDLLQEVFEARPEVFAHNLETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETVD
EIRDALVDLHSAGCDIITITQYLRPGPMYHPIDRWVKPEEFIDHADFARELGFGAVMSGPLVRSSYRAGKLYAEALAARG
ESLPENLAHLATTADGSTAQEANTLLEKYGPSQDTPVVSSKA
>Mature_361_residues
STTTAITSTLQRVTKAPEGRRMLRVEARNSQTPIESKPRWIRTAVKTGPEYQDMKKKVSGASLHTVCQEAGCPNIHECWE
SREATFLIGGANCSRRCDFCQINSAKPEPLDRDEPRRVAESVREMQLNYSTITGVTRDDLEDEGAWLYAEVVRKIHELNP
HTGVENLTPDFSGKPDLLQEVFEARPEVFAHNLETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETVDE
IRDALVDLHSAGCDIITITQYLRPGPMYHPIDRWVKPEEFIDHADFARELGFGAVMSGPLVRSSYRAGKLYAEALAARGE
SLPENLAHLATTADGSTAQEANTLLEKYGPSQDTPVVSSKA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=37.2413793103448, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI37577164, Length=236, Percent_Identity=37.2881355932203, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786846, Length=306, Percent_Identity=37.5816993464052, Blast_Score=221, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=39.84375, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6324770, Length=300, Percent_Identity=38.6666666666667, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=39.3728222996516, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 40484; Mature: 40353

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTAITSTLQRVTKAPEGRRMLRVEARNSQTPIESKPRWIRTAVKTGPEYQDMKKKVS
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHC
GASLHTVCQEAGCPNIHECWESREATFLIGGANCSRRCDFCQINSAKPEPLDRDEPRRVA
CCHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHH
ESVREMQLNYSTITGVTRDDLEDEGAWLYAEVVRKIHELNPHTGVENLTPDFSGKPDLLQ
HHHHHHHHCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
EVFEARPEVFAHNLETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETVD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCHHHH
EIRDALVDLHSAGCDIITITQYLRPGPMYHPIDRWVKPEEFIDHADFARELGFGAVMSGP
HHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHCCC
LVRSSYRAGKLYAEALAARGESLPENLAHLATTADGSTAQEANTLLEKYGPSQDTPVVSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
KA
CC
>Mature Secondary Structure 
STTTAITSTLQRVTKAPEGRRMLRVEARNSQTPIESKPRWIRTAVKTGPEYQDMKKKVS
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHC
GASLHTVCQEAGCPNIHECWESREATFLIGGANCSRRCDFCQINSAKPEPLDRDEPRRVA
CCHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHH
ESVREMQLNYSTITGVTRDDLEDEGAWLYAEVVRKIHELNPHTGVENLTPDFSGKPDLLQ
HHHHHHHHCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
EVFEARPEVFAHNLETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETVD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCHHHH
EIRDALVDLHSAGCDIITITQYLRPGPMYHPIDRWVKPEEFIDHADFARELGFGAVMSGP
HHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHCCC
LVRSSYRAGKLYAEALAARGESLPENLAHLATTADGSTAQEANTLLEKYGPSQDTPVVSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]