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The map label for this gene is glgX [H]

Identifier: 38234147

GI number: 38234147

Start: 1597883

End: 1600090

Strand: Direct

Name: glgX [H]

Synonym: DIP1572

Alternate gene names: 38234147

Gene position: 1597883-1600090 (Clockwise)

Preceding gene: 38234146

Following gene: 38234148

Centisome position: 64.21

GC content: 52.63

Gene sequence:

>2208_bases
ATGAACGACTCCCCTGTGACGTCCGATCGTCAAATCTGGCCCGGACAAAGCTACCCACTGGGCTCTAAATACGACGGAGC
AGGTACGAACTTTGCTATCTTCTCTGACGTTGCCGAAAAAGTTGAGCTCTGCCTCATCGACGCAGAGTGCAATGAGGAAA
GAATCCTCCTCGACGAAGTTGATGCCCACATCTGGCACTGCTACCTCCCAGGCGTCAAACCTGGCCAACGCTATGGCTTC
CGCGTCCATGGCCCCTATAACCCTGCTGAAGGCAAACGTTGCGATCCCAGCAAACTACTAGTCGATCCATATGCACGCGC
TTTTGACGGTGAATTCGATGGACATCCATCGCTGTTTAGTTATGACATCAACGATCCAGAAAATCCCGAGGGTCGCAATA
CTGAAGATAGTCTCGAGCACACCATGAAGTCGGTTGTAGTGAATCCTTTCTTTGACTGGGGTTCAGACCGCAGCCCAAAC
ACGCCGTACAACGAGACAGTAATCTACGAAGCTCATGTCAAGGGCATGACGATGCGCCACCCAGACGTACCCGAATCCCT
CCGTGGTACTTATGCTGGTCTAGCGCATCCTTCGATCATCGAACATCTCAAAGACCTTGGCATCACTGCAATCGAGCTCA
TGCCAGTGCATCAGTTCCTGCAAGATGACCGCCTCCGCGACTTGGGATTACGTAACTATTGGGGCTATAACACCTTTGGT
TTCTTTGCACCTCAACAAGATTATGCGGCGGCCCAAGAGCCAGGTGGGGCTGTCTCCGAGTTCAAAGGAATGGTGCGCGC
TTTCCACGAAGCAGGCATCGAGGTCATCCTCGACGTGGTCTATAACCACACCGCGGAGGGAAACCACATGGGTCCCACGA
TCGCATTCCGCGGCATCGACAATGAGGCATACTACCGCCTCGTTGACGGAGATAAAGCCCACTACATGGACTACACGGGC
ACCGGAAATTCACTCAACGTCCGAGATCCGCATCCATTGCAAATGATCATGGATTCCCTGCGTTACTGGGTCTCAGAGAT
GCACGTTGATGGTTTCCGATTCGACTTAGCTTCCACTTTGGCTCGTGAACTACACGACGTGGATAAGCTCGCTACATTCT
TCGATCTTGTCCAACAAGATCCGATTGTTTCTCAAGTCAAACTCATCGCCGAGCCTTGGGATGTGGGCGAGGGCGGTTAC
CAAGTGGGTAATTTCCCGCCTCTTTGGACTGAGTGGAATGGAAAATACCGCGATACTGTCCGTGATTTCTGGCGTGGTGA
GCCGTCGACACTCGGCGAATTTGCTTCTCGTCTTACTGGCTCTTCAGACCTATACGCCAATAATGGTCGCCGCCCTACGG
CTTCTATCAACTTCATCACGGCTCACGACGGCTTCACGCTCAATGACCTAGTCAGCTACAACCACAAGCACAACGATGCA
AATGGAGAAGACGGTCGGGATGGGGAAAGCCACAATCGTTCATGGAACTGCGGCGAAGAAGGCCCCACCGAAGATCCTAG
GATCAACAAATTACGTGCACGTCAACGACGTAATTTCCTCACAACGCTTTTGCTCAGCCAAGGCACCCCCATGATTGCCC
ATGGCGATGAAATGGCGCGTACTCAAGATGGCAATAACAATGTTTACTGCCAAGACAATGAGACGTCGTGGATGGATTGG
GAGCTAGCAGACAAAAACGCTGCGTTGATGGAGTTCACCAAACGGCTCATTACGATCCGCCGTAACCATCCTGTGTTTAG
ACGCCGGCGCTTCTTGGCTGGGGGTCCGTTGGGATCAGAAGTAGGCGACCGCGATATTGCGTGGCTGGTTCCTTCTGGCA
AGTTGATGGGTCAAAGCGACTGGGATTTCGCCTTTGGCAAGTCCCTCATGGTCTACTTCAATGGCAAGGCCATCCAAGAA
CCCGATGCGCGCGGCCAACGCATTGAAGGTGACTCCTTCATCATGATGTTCAATGCCTATCATGAGCCGATCGACTTTAC
TCTTCCTGACACCGAGTTCGGTCCAGCGTGGAAACTTATCGTCGATACCAATGTTGATACTGGCTACCCTGACGAAGCAA
AGACGCTTCGCGCAGGCGACTCGATTACCGTCGAAGCACGCAGCACGCTGATCCTTCGACTCGTCGACACCACACATAAG
TCCCAGACTGTGGAAAAGGATCGTCGAGACGACGACTACAGTGACTAG

Upstream 100 bases:

>100_bases
CCGTTAGGAACCACGATCAACTCTTTATGAGCAGCCTTGAGATTATTATCGCTGGATTGAAATCACAGGGGTTTTCCTAA
AAGCAGTAGAGTGAGTAGCT

Downstream 100 bases:

>100_bases
TTAACGTCGACACGCACTGCGGCGCCCGATCATGCGATGACACGTTCGAGCGCCGCAGTGAGCATGTCACTGCGCACCTG
TAGTGTCAGACATCTGAGCA

Product: glycogen operon protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MNDSPVTSDRQIWPGQSYPLGSKYDGAGTNFAIFSDVAEKVELCLIDAECNEERILLDEVDAHIWHCYLPGVKPGQRYGF
RVHGPYNPAEGKRCDPSKLLVDPYARAFDGEFDGHPSLFSYDINDPENPEGRNTEDSLEHTMKSVVVNPFFDWGSDRSPN
TPYNETVIYEAHVKGMTMRHPDVPESLRGTYAGLAHPSIIEHLKDLGITAIELMPVHQFLQDDRLRDLGLRNYWGYNTFG
FFAPQQDYAAAQEPGGAVSEFKGMVRAFHEAGIEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVDGDKAHYMDYTG
TGNSLNVRDPHPLQMIMDSLRYWVSEMHVDGFRFDLASTLARELHDVDKLATFFDLVQQDPIVSQVKLIAEPWDVGEGGY
QVGNFPPLWTEWNGKYRDTVRDFWRGEPSTLGEFASRLTGSSDLYANNGRRPTASINFITAHDGFTLNDLVSYNHKHNDA
NGEDGRDGESHNRSWNCGEEGPTEDPRINKLRARQRRNFLTTLLLSQGTPMIAHGDEMARTQDGNNNVYCQDNETSWMDW
ELADKNAALMEFTKRLITIRRNHPVFRRRRFLAGGPLGSEVGDRDIAWLVPSGKLMGQSDWDFAFGKSLMVYFNGKAIQE
PDARGQRIEGDSFIMMFNAYHEPIDFTLPDTEFGPAWKLIVDTNVDTGYPDEAKTLRAGDSITVEARSTLILRLVDTTHK
SQTVEKDRRDDDYSD

Sequences:

>Translated_735_residues
MNDSPVTSDRQIWPGQSYPLGSKYDGAGTNFAIFSDVAEKVELCLIDAECNEERILLDEVDAHIWHCYLPGVKPGQRYGF
RVHGPYNPAEGKRCDPSKLLVDPYARAFDGEFDGHPSLFSYDINDPENPEGRNTEDSLEHTMKSVVVNPFFDWGSDRSPN
TPYNETVIYEAHVKGMTMRHPDVPESLRGTYAGLAHPSIIEHLKDLGITAIELMPVHQFLQDDRLRDLGLRNYWGYNTFG
FFAPQQDYAAAQEPGGAVSEFKGMVRAFHEAGIEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVDGDKAHYMDYTG
TGNSLNVRDPHPLQMIMDSLRYWVSEMHVDGFRFDLASTLARELHDVDKLATFFDLVQQDPIVSQVKLIAEPWDVGEGGY
QVGNFPPLWTEWNGKYRDTVRDFWRGEPSTLGEFASRLTGSSDLYANNGRRPTASINFITAHDGFTLNDLVSYNHKHNDA
NGEDGRDGESHNRSWNCGEEGPTEDPRINKLRARQRRNFLTTLLLSQGTPMIAHGDEMARTQDGNNNVYCQDNETSWMDW
ELADKNAALMEFTKRLITIRRNHPVFRRRRFLAGGPLGSEVGDRDIAWLVPSGKLMGQSDWDFAFGKSLMVYFNGKAIQE
PDARGQRIEGDSFIMMFNAYHEPIDFTLPDTEFGPAWKLIVDTNVDTGYPDEAKTLRAGDSITVEARSTLILRLVDTTHK
SQTVEKDRRDDDYSD
>Mature_735_residues
MNDSPVTSDRQIWPGQSYPLGSKYDGAGTNFAIFSDVAEKVELCLIDAECNEERILLDEVDAHIWHCYLPGVKPGQRYGF
RVHGPYNPAEGKRCDPSKLLVDPYARAFDGEFDGHPSLFSYDINDPENPEGRNTEDSLEHTMKSVVVNPFFDWGSDRSPN
TPYNETVIYEAHVKGMTMRHPDVPESLRGTYAGLAHPSIIEHLKDLGITAIELMPVHQFLQDDRLRDLGLRNYWGYNTFG
FFAPQQDYAAAQEPGGAVSEFKGMVRAFHEAGIEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVDGDKAHYMDYTG
TGNSLNVRDPHPLQMIMDSLRYWVSEMHVDGFRFDLASTLARELHDVDKLATFFDLVQQDPIVSQVKLIAEPWDVGEGGY
QVGNFPPLWTEWNGKYRDTVRDFWRGEPSTLGEFASRLTGSSDLYANNGRRPTASINFITAHDGFTLNDLVSYNHKHNDA
NGEDGRDGESHNRSWNCGEEGPTEDPRINKLRARQRRNFLTTLLLSQGTPMIAHGDEMARTQDGNNNVYCQDNETSWMDW
ELADKNAALMEFTKRLITIRRNHPVFRRRRFLAGGPLGSEVGDRDIAWLVPSGKLMGQSDWDFAFGKSLMVYFNGKAIQE
PDARGQRIEGDSFIMMFNAYHEPIDFTLPDTEFGPAWKLIVDTNVDTGYPDEAKTLRAGDSITVEARSTLILRLVDTTHK
SQTVEKDRRDDDYSD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=664, Percent_Identity=46.0843373493976, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83218; Mature: 83218

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSPVTSDRQIWPGQSYPLGSKYDGAGTNFAIFSDVAEKVELCLIDAECNEERILLDEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCCEEEHHHH
DAHIWHCYLPGVKPGQRYGFRVHGPYNPAEGKRCDPSKLLVDPYARAFDGEFDGHPSLFS
CCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCEEE
YDINDPENPEGRNTEDSLEHTMKSVVVNPFFDWGSDRSPNTPYNETVIYEAHVKGMTMRH
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECC
PDVPESLRGTYAGLAHPSIIEHLKDLGITAIELMPVHQFLQDDRLRDLGLRNYWGYNTFG
CCCCHHHHCCHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHHCCCCCCCCCCCEE
FFAPQQDYAAAQEPGGAVSEFKGMVRAFHEAGIEVILDVVYNHTAEGNHMGPTIAFRGID
ECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECC
NEAYYRLVDGDKAHYMDYTGTGNSLNVRDPHPLQMIMDSLRYWVSEMHVDGFRFDLASTL
CCEEEEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARELHDVDKLATFFDLVQQDPIVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWNGKYRDTV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHH
RDFWRGEPSTLGEFASRLTGSSDLYANNGRRPTASINFITAHDGFTLNDLVSYNHKHNDA
HHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCC
NGEDGRDGESHNRSWNCGEEGPTEDPRINKLRARQRRNFLTTLLLSQGTPMIAHGDEMAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
TQDGNNNVYCQDNETSWMDWELADKNAALMEFTKRLITIRRNHPVFRRRRFLAGGPLGSE
CCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCC
VGDRDIAWLVPSGKLMGQSDWDFAFGKSLMVYFNGKAIQEPDARGQRIEGDSFIMMFNAY
CCCCCEEEEECCCCCCCCCCCCHHCCCEEEEEECCCEECCCCCCCCEECCCCEEEEEECC
HEPIDFTLPDTEFGPAWKLIVDTNVDTGYPDEAKTLRAGDSITVEARSTLILRLVDTTHK
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHEECCCCEEEEECCEEEEEEECCCCC
SQTVEKDRRDDDYSD
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDSPVTSDRQIWPGQSYPLGSKYDGAGTNFAIFSDVAEKVELCLIDAECNEERILLDEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCCEEEHHHH
DAHIWHCYLPGVKPGQRYGFRVHGPYNPAEGKRCDPSKLLVDPYARAFDGEFDGHPSLFS
CCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCEEE
YDINDPENPEGRNTEDSLEHTMKSVVVNPFFDWGSDRSPNTPYNETVIYEAHVKGMTMRH
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECC
PDVPESLRGTYAGLAHPSIIEHLKDLGITAIELMPVHQFLQDDRLRDLGLRNYWGYNTFG
CCCCHHHHCCHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHHCCCCCCCCCCCEE
FFAPQQDYAAAQEPGGAVSEFKGMVRAFHEAGIEVILDVVYNHTAEGNHMGPTIAFRGID
ECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECC
NEAYYRLVDGDKAHYMDYTGTGNSLNVRDPHPLQMIMDSLRYWVSEMHVDGFRFDLASTL
CCEEEEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARELHDVDKLATFFDLVQQDPIVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWNGKYRDTV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHH
RDFWRGEPSTLGEFASRLTGSSDLYANNGRRPTASINFITAHDGFTLNDLVSYNHKHNDA
HHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCC
NGEDGRDGESHNRSWNCGEEGPTEDPRINKLRARQRRNFLTTLLLSQGTPMIAHGDEMAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
TQDGNNNVYCQDNETSWMDWELADKNAALMEFTKRLITIRRNHPVFRRRRFLAGGPLGSE
CCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCC
VGDRDIAWLVPSGKLMGQSDWDFAFGKSLMVYFNGKAIQEPDARGQRIEGDSFIMMFNAY
CCCCCEEEEECCCCCCCCCCCCHHCCCEEEEEECCCEECCCCCCCCEECCCCEEEEEECC
HEPIDFTLPDTEFGPAWKLIVDTNVDTGYPDEAKTLRAGDSITVEARSTLILRLVDTTHK
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHEECCCCEEEEECCEEEEEEECCCCC
SQTVEKDRRDDDYSD
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]