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The map label for this gene is rlmN

Identifier: 38234081

GI number: 38234081

Start: 1526585

End: 1527691

Strand: Reverse

Name: rlmN

Synonym: DIP1502

Alternate gene names: 38234081

Gene position: 1527691-1526585 (Counterclockwise)

Preceding gene: 38234083

Following gene: 38234079

Centisome position: 61.39

GC content: 50.95

Gene sequence:

>1107_bases
ATGACGAATCCAACGAAGCCCATTCCGCTAAATTTTGATAAGCCTCGGCGGTTCATGCCCCCGAAGCACTTTGCTGATCT
TTCTGCTGACGAGCGTATCGACGCTCTTAAAGAGCTGGGTTTGCCAAAATTCCGTGCTAATCAGATTGCTCGGCATTATT
ATGGTCGCCTTGAAGCTGATCCTAGTACTATGACTGATTTGCCTGCGGCAGCAAGAGAAAAAGTAAAAGATGCGCTTTTT
CCGCAGTTGATGCAGCCGGTGCGTGCTGTTCAAGCAGATGATGGCGAAACTCAAAAAACTTTGTGGAAACTCCATGATGG
GACTCTCTTAGAGTCAGTGCTGATGCGTTATCCTAACCGCGCAACGTTGTGTATTTCTTCCCAAGCTGGTTGCGGTATGG
CTTGTCCGTTTTGTGCCACGGGCCAAGGGGGACTCGATCGTAACTTGTCTACTGGCGAAATCGTCGATCAGGTTCGTGCT
GCGTCAGCGACGATGCAGGCTGAGGGCGGGCGATTGTCCAACATTGTCTTTATGGGGATGGGGGAGCCTCTCGCTAACTA
CAAGCGCGTCGTCTCAGCAGTTCGGCAAATTACAGCACCGGTACCGGAAGGATTTGGTATTTCCCAACGAAATGTCACGG
TATCCACCGTGGGTCTGGCTCCTGCCATTCGAAAGCTTGCGGATGAGGATCTTTCGGTCACTTTGGCAGTATCACTACAT
ACACCAGACGATGAATTGCGTAACACCTTGGTTCCCACGAACAATCGCTGGGAAGTTGCTGAGGTTCTAGATGCGGCTCG
TTACTATGCTGACCGATCTGGGCGACGCGTCTCGATTGAATATGCACTGATTCGCGACGTCAACGATCAGGGATGGCGTG
CGGATATGTTGGGTAAGAAATTGCATAAAGCTCTTGGGCCATTGGTACACGTGAATCTTATTCCGCTTAATCCTACACCT
GGGTCGAAGTGGGACGCATCGCCCATGGATCGTCAAAAGGAATTCGTCCAACGTGTTATTGCTCAAGGAGTGACGTGTAC
AGTGCGTGATACTCGTGGGCAAGAAATTGCGGCTGCGTGTGGTCAGCTTGCTGCAGAAGAGCGTTAG

Upstream 100 bases:

>100_bases
TGCAAGCATTGTGTTTCATGGCGTTTTATAAAAGGGAAAACTCCAAAACTAAGTGGTCAGTTCACAATTATCGCGCGTAA
CGTGTCATGATGGTAGCAAT

Downstream 100 bases:

>100_bases
CTCTTCGAAAAATGTAGGGGGCGCATAAAAAAGCTTCCGCATCTGTGGATATAGATGCGGAAGCTTTTAGTAATTCGATT
TAAGCCTCTGGGAGAGTGCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTNPTKPIPLNFDKPRRFMPPKHFADLSADERIDALKELGLPKFRANQIARHYYGRLEADPSTMTDLPAAAREKVKDALF
PQLMQPVRAVQADDGETQKTLWKLHDGTLLESVLMRYPNRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRA
ASATMQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITAPVPEGFGISQRNVTVSTVGLAPAIRKLADEDLSVTLAVSLH
TPDDELRNTLVPTNNRWEVAEVLDAARYYADRSGRRVSIEYALIRDVNDQGWRADMLGKKLHKALGPLVHVNLIPLNPTP
GSKWDASPMDRQKEFVQRVIAQGVTCTVRDTRGQEIAAACGQLAAEER

Sequences:

>Translated_368_residues
MTNPTKPIPLNFDKPRRFMPPKHFADLSADERIDALKELGLPKFRANQIARHYYGRLEADPSTMTDLPAAAREKVKDALF
PQLMQPVRAVQADDGETQKTLWKLHDGTLLESVLMRYPNRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRA
ASATMQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITAPVPEGFGISQRNVTVSTVGLAPAIRKLADEDLSVTLAVSLH
TPDDELRNTLVPTNNRWEVAEVLDAARYYADRSGRRVSIEYALIRDVNDQGWRADMLGKKLHKALGPLVHVNLIPLNPTP
GSKWDASPMDRQKEFVQRVIAQGVTCTVRDTRGQEIAAACGQLAAEER
>Mature_367_residues
TNPTKPIPLNFDKPRRFMPPKHFADLSADERIDALKELGLPKFRANQIARHYYGRLEADPSTMTDLPAAAREKVKDALFP
QLMQPVRAVQADDGETQKTLWKLHDGTLLESVLMRYPNRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAA
SATMQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITAPVPEGFGISQRNVTVSTVGLAPAIRKLADEDLSVTLAVSLHT
PDDELRNTLVPTNNRWEVAEVLDAARYYADRSGRRVSIEYALIRDVNDQGWRADMLGKKLHKALGPLVHVNLIPLNPTPG
SKWDASPMDRQKEFVQRVIAQGVTCTVRDTRGQEIAAACGQLAAEER

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=38.6363636363636, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CORDI (Q6NGK9)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939848.1
- ProteinModelPortal:   Q6NGK9
- GeneID:   2650386
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1502
- NMPDR:   fig|257309.1.peg.1439
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q6NGK9
- ProtClustDB:   PRK14459
- BioCyc:   CDIP257309:DIP1502-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40440; Mature: 40309

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPTKPIPLNFDKPRRFMPPKHFADLSADERIDALKELGLPKFRANQIARHYYGRLEAD
CCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PSTMTDLPAAAREKVKDALFPQLMQPVRAVQADDGETQKTLWKLHDGTLLESVLMRYPNR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCC
ATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAASATMQAEGGRLSNIVFMGM
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
GEPLANYKRVVSAVRQITAPVPEGFGISQRNVTVSTVGLAPAIRKLADEDLSVTLAVSLH
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEE
TPDDELRNTLVPTNNRWEVAEVLDAARYYADRSGRRVSIEYALIRDVNDQGWRADMLGKK
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHH
LHKALGPLVHVNLIPLNPTPGSKWDASPMDRQKEFVQRVIAQGVTCTVRDTRGQEIAAAC
HHHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
GQLAAEER
HHHHCCCC
>Mature Secondary Structure 
TNPTKPIPLNFDKPRRFMPPKHFADLSADERIDALKELGLPKFRANQIARHYYGRLEAD
CCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PSTMTDLPAAAREKVKDALFPQLMQPVRAVQADDGETQKTLWKLHDGTLLESVLMRYPNR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCC
ATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAASATMQAEGGRLSNIVFMGM
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
GEPLANYKRVVSAVRQITAPVPEGFGISQRNVTVSTVGLAPAIRKLADEDLSVTLAVSLH
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEE
TPDDELRNTLVPTNNRWEVAEVLDAARYYADRSGRRVSIEYALIRDVNDQGWRADMLGKK
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHH
LHKALGPLVHVNLIPLNPTPGSKWDASPMDRQKEFVQRVIAQGVTCTVRDTRGQEIAAAC
HHHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
GQLAAEER
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910