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The map label for this gene is proS

Identifier: 38234063

GI number: 38234063

Start: 1502667

End: 1504424

Strand: Reverse

Name: proS

Synonym: DIP1482

Alternate gene names: 38234063

Gene position: 1504424-1502667 (Counterclockwise)

Preceding gene: 38234065

Following gene: 38234061

Centisome position: 60.45

GC content: 51.99

Gene sequence:

>1758_bases
ATGATTACACGTCTTTCTACACTTTTCCTTCGCACTCTGCGCGAAGACCCTGCCGATGCAGAAGTACCAAGCCACAAGTT
GCTTGTTCGTGCGGGATACATTCGTCGAACAGCTCCTGGTGTATATACTTGGTTGCCGCTGGGACTTCGCACGTTGCGCA
AAGTAGAAACTGTCGTGCGAGAAGAAATGGATGCCATTGGAGCGCAAGAGCTTTTGTTCCCTGCGCTGTTGCCTCGTGAG
CCATATGAGCAGACACATCGTTGGACCGAATACGGTGATTCCCTATTCCGCCTGAAAGACCGTAAAGGTGGCGACTATCT
TCTCGGGCCAACGCATGAAGAAATGTTCGCCTCGGCTGTCAAGGACATGTATTCGTCCTATAAGGATTTCCCAGTCACCT
TGTATCAGATCCAGACAAAGTATCGTGATGAAGAGCGCCCTCGTGCCGGTATTCTTCGTGGCCGAGAGTTTGTTATGAAG
GATTCTTATTCCTTTGATATGTCTGACGCTGGGCTAGAAGATTCGTATCAGCGCCATCGTGAGGCATATCAGCGAATCCT
CGATCGTCTCGGTGTGGAATACGTTATTTGTGCTGCAACTTCGGGCGCTATGGGAGGCTCGGCTTCAGAAGAGTTCCTTG
CTGTAAGTGACAATGGCGAAGATACTTTCGTACGTGCCACTGAAGGGCCATATGCTGCGAACGTCGAGGCCGTAGTTACT
CAGCCGGGAGTAGAGCGTCCGCTCGAACAAGCACCCGAGGCCGTTGAGTACGAAACGCCTAATGCTGAAACCATTGAAGC
GCTAGTTCAATGGGCACAGTCTGCTGGCGTTACCGTTGAAGATCGTTCTGTTGCAGCGGCAGACACATTGAAGTGTTTGT
TGGTTAAGATCACCCAGCCTGGTGCTGAAGAAGCAGAGCTTGCGGGCATTTTATTGCCAGGTGATCGTGAAGTAGACATG
AAGCGCCTTGAAGCCTCCGTCGAGCCTGCTGAAGTTGAATTAGCCTCCGAAGAGGACTTCAAGAACAATCCATTCTTGGT
CAAGGGCTACGTTGGCCCACGGGCTTTGAATGCCCACGGTGTGAAGGTATTGGCTGATCCCCGCGTTGTGAGTGGAACTT
CATGGATTGCCGGTGCAGATGCCGTTGAACACCATGTTGTTGGCTTGACTATGGGCCGTGACTTTACGGTTGATGGATAC
ATCGAGGCTGCTGAAATTCGTGAGGGTGACCCAGCGCCAGAGGGACAAGGTACTTTGACACTTGCGCGTGGCATTGAGGT
TGGACACATCTTCCAGCTGGGGCGCAAGTACACAGAGGCATTCGATGTTCAGATCTTGGACGAGTCCGGTAAGCGCGCCA
TCCCTACGATGGGTTCCTACGGCATTGGTGTTTCTCGATTGATGGCTGTTCTTGCCGAGCAGCGCCATGATGAAACTGGC
CTGAACTGGCCGCTCGAGGTTGCTCCTTACCAAGTGCATGTTGTGGTTGCCAACAAAGACAAGGAAGCTATTGAAGCAGG
CGATGCACTTGTGGCAGCTCTTGACTCACATGGCATTGAGGTGTTGTTCGATGATCGCCCTAAGGTGAGCCCTGGCGTGA
AGTTCAAAGACGCTGAACTACTCGGCATGCCATTTGTTGTGGTCTTGGGTCGTGCATTCAAAGATGGCAACATTGAGCTA
CGTGAACGTGGCCAGGAAACAGTCTTGGTGTCGGCAGATGAGATCGTCGATACGGTCGTGGCTAAACTGAACCGTTAG

Upstream 100 bases:

>100_bases
ATCGAAATCGATAGCTGGATTCTTTCCCCCAACAGCCTTCGTCTCGGAAGGTGGCAACACAATGAGCATGCCTACATCCT
AACGGGTATATTAAGCCTTT

Downstream 100 bases:

>100_bases
TAGCGTAATGGCGCTGCAGCTGCAATTCTAGAGCCTCTCTCGAGATCGTAGTCCCGAGAGAGGCTCTAGTGTTTTTTCTT
AACGTCGAGCTGCACTTTGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRTAPGVYTWLPLGLRTLRKVETVVREEMDAIGAQELLFPALLPRE
PYEQTHRWTEYGDSLFRLKDRKGGDYLLGPTHEEMFASAVKDMYSSYKDFPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDMSDAGLEDSYQRHREAYQRILDRLGVEYVICAATSGAMGGSASEEFLAVSDNGEDTFVRATEGPYAANVEAVVT
QPGVERPLEQAPEAVEYETPNAETIEALVQWAQSAGVTVEDRSVAAADTLKCLLVKITQPGAEEAELAGILLPGDREVDM
KRLEASVEPAEVELASEEDFKNNPFLVKGYVGPRALNAHGVKVLADPRVVSGTSWIAGADAVEHHVVGLTMGRDFTVDGY
IEAAEIREGDPAPEGQGTLTLARGIEVGHIFQLGRKYTEAFDVQILDESGKRAIPTMGSYGIGVSRLMAVLAEQRHDETG
LNWPLEVAPYQVHVVVANKDKEAIEAGDALVAALDSHGIEVLFDDRPKVSPGVKFKDAELLGMPFVVVLGRAFKDGNIEL
RERGQETVLVSADEIVDTVVAKLNR

Sequences:

>Translated_585_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRTAPGVYTWLPLGLRTLRKVETVVREEMDAIGAQELLFPALLPRE
PYEQTHRWTEYGDSLFRLKDRKGGDYLLGPTHEEMFASAVKDMYSSYKDFPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDMSDAGLEDSYQRHREAYQRILDRLGVEYVICAATSGAMGGSASEEFLAVSDNGEDTFVRATEGPYAANVEAVVT
QPGVERPLEQAPEAVEYETPNAETIEALVQWAQSAGVTVEDRSVAAADTLKCLLVKITQPGAEEAELAGILLPGDREVDM
KRLEASVEPAEVELASEEDFKNNPFLVKGYVGPRALNAHGVKVLADPRVVSGTSWIAGADAVEHHVVGLTMGRDFTVDGY
IEAAEIREGDPAPEGQGTLTLARGIEVGHIFQLGRKYTEAFDVQILDESGKRAIPTMGSYGIGVSRLMAVLAEQRHDETG
LNWPLEVAPYQVHVVVANKDKEAIEAGDALVAALDSHGIEVLFDDRPKVSPGVKFKDAELLGMPFVVVLGRAFKDGNIEL
RERGQETVLVSADEIVDTVVAKLNR
>Mature_585_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRTAPGVYTWLPLGLRTLRKVETVVREEMDAIGAQELLFPALLPRE
PYEQTHRWTEYGDSLFRLKDRKGGDYLLGPTHEEMFASAVKDMYSSYKDFPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDMSDAGLEDSYQRHREAYQRILDRLGVEYVICAATSGAMGGSASEEFLAVSDNGEDTFVRATEGPYAANVEAVVT
QPGVERPLEQAPEAVEYETPNAETIEALVQWAQSAGVTVEDRSVAAADTLKCLLVKITQPGAEEAELAGILLPGDREVDM
KRLEASVEPAEVELASEEDFKNNPFLVKGYVGPRALNAHGVKVLADPRVVSGTSWIAGADAVEHHVVGLTMGRDFTVDGY
IEAAEIREGDPAPEGQGTLTLARGIEVGHIFQLGRKYTEAFDVQILDESGKRAIPTMGSYGIGVSRLMAVLAEQRHDETG
LNWPLEVAPYQVHVVVANKDKEAIEAGDALVAALDSHGIEVLFDDRPKVSPGVKFKDAELLGMPFVVVLGRAFKDGNIEL
RERGQETVLVSADEIVDTVVAKLNR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=244, Percent_Identity=38.1147540983607, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1786392, Length=589, Percent_Identity=40.7470288624788, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI115532348, Length=215, Percent_Identity=35.3488372093023, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320931, Length=567, Percent_Identity=28.7477954144621, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24656200, Length=216, Percent_Identity=34.7222222222222, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CORDI (Q6NGM7)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939830.1
- ProteinModelPortal:   Q6NGM7
- SMR:   Q6NGM7
- GeneID:   2650367
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1482
- NMPDR:   fig|257309.1.peg.1421
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q6NGM7
- ProtClustDB:   PRK09194
- BioCyc:   CDIP257309:DIP1482-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64467; Mature: 64467

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRTAPGVYTWLPLGLRTLRKVETVVR
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHCCCCCEEHHHHHHHHHHHHHHHHH
EEMDAIGAQELLFPALLPREPYEQTHRWTEYGDSLFRLKDRKGGDYLLGPTHEEMFASAV
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHH
KDMYSSYKDFPVTLYQIQTKYRDEERPRAGILRGREFVMKDSYSFDMSDAGLEDSYQRHR
HHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHH
EAYQRILDRLGVEYVICAATSGAMGGSASEEFLAVSDNGEDTFVRATEGPYAANVEAVVT
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCEEEEE
QPGVERPLEQAPEAVEYETPNAETIEALVQWAQSAGVTVEDRSVAAADTLKCLLVKITQP
CCCCCCHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHEEEEEEECCC
GAEEAELAGILLPGDREVDMKRLEASVEPAEVELASEEDFKNNPFLVKGYVGPRALNAHG
CCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCCEECCCC
VKVLADPRVVSGTSWIAGADAVEHHVVGLTMGRDFTVDGYIEAAEIREGDPAPEGQGTLT
EEEEECCEEECCCCCCCCCHHHHHEEEEEEECCCEEECCEEEEECCCCCCCCCCCCCCEE
LARGIEVGHIFQLGRKYTEAFDVQILDESGKRAIPTMGSYGIGVSRLMAVLAEQRHDETG
EECCCCHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCC
LNWPLEVAPYQVHVVVANKDKEAIEAGDALVAALDSHGIEVLFDDRPKVSPGVKFKDAEL
CCCCEEECCEEEEEEEECCCHHHHHHCCHHEEEECCCCEEEEECCCCCCCCCCEECCCHH
LGMPFVVVLGRAFKDGNIELRERGQETVLVSADEIVDTVVAKLNR
CCCCHHHHHHHHCCCCCCHHCCCCCEEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRTAPGVYTWLPLGLRTLRKVETVVR
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHCCCCCEEHHHHHHHHHHHHHHHHH
EEMDAIGAQELLFPALLPREPYEQTHRWTEYGDSLFRLKDRKGGDYLLGPTHEEMFASAV
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHH
KDMYSSYKDFPVTLYQIQTKYRDEERPRAGILRGREFVMKDSYSFDMSDAGLEDSYQRHR
HHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHH
EAYQRILDRLGVEYVICAATSGAMGGSASEEFLAVSDNGEDTFVRATEGPYAANVEAVVT
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCEEEEE
QPGVERPLEQAPEAVEYETPNAETIEALVQWAQSAGVTVEDRSVAAADTLKCLLVKITQP
CCCCCCHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHEEEEEEECCC
GAEEAELAGILLPGDREVDMKRLEASVEPAEVELASEEDFKNNPFLVKGYVGPRALNAHG
CCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCCEECCCC
VKVLADPRVVSGTSWIAGADAVEHHVVGLTMGRDFTVDGYIEAAEIREGDPAPEGQGTLT
EEEEECCEEECCCCCCCCCHHHHHEEEEEEECCCEEECCEEEEECCCCCCCCCCCCCCEE
LARGIEVGHIFQLGRKYTEAFDVQILDESGKRAIPTMGSYGIGVSRLMAVLAEQRHDETG
EECCCCHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCC
LNWPLEVAPYQVHVVVANKDKEAIEAGDALVAALDSHGIEVLFDDRPKVSPGVKFKDAEL
CCCCEEECCEEEEEEEECCCHHHHHHCCHHEEEECCCCEEEEECCCCCCCCCCEECCCHH
LGMPFVVVLGRAFKDGNIELRERGQETVLVSADEIVDTVVAKLNR
CCCCHHHHHHHHCCCCCCHHCCCCCEEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910