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The map label for this gene is rotA [H]

Identifier: 37680779

GI number: 37680779

Start: 2622349

End: 2622894

Strand: Direct

Name: rotA [H]

Synonym: VV2595

Alternate gene names: 37680779

Gene position: 2622349-2622894 (Clockwise)

Preceding gene: 37680778

Following gene: 37680780

Centisome position: 78.17

GC content: 46.15

Gene sequence:

>546_bases
ATGCTCAAAGTTATCTGTGCATCACTCGTACTCACGAGTAGCCTCGCCTTGGCGGGGCCAAAAGTTGAATTCGAAACCTC
TCTGGGGAATTTCACTGTTGAGTTAAATGAAGAAAAAGCGCCGATTAGCGTCGAAAACTTCGTAAAATACGTGAAAGATG
GCAGTTACGTAGGATCTCAATTTCACCGTGTCATTCCTGGTTTTATGGCGCAAGGTGGTGGTTTTGATGAGAAAATGAAT
CGTCTACCCAGTTACGCACCAATAAAGAACGAAGCAAGTAATGGTCTGAAGAACGTGACGGGCTCTATCGCAATGGCTCG
AACTCAAGATCCTAACTCTGCGACACGACAATTCTTTATCAACTTGGTGGATAACGAGTTTCTCGACTATGACCTCCAAC
CTCCAGGTTACGCCGTTTTTGGTCAAGTCACCGAAGGGTTTGACGTCATTCAACAGATGGCGAGCAAACCGACCAAAAGC
CAAGGCTACATGAGAGATATTCCCGTGGAACCAATCGTGATCACCAAGGCCACACTACTCCCATAA

Upstream 100 bases:

>100_bases
ATCAAGAAGTGGAAAAAGTGCTAAATGATGTGATCACATTAGTCCTTGCTGAGATTGCCAATGACCAAGAACTGCAAAGC
TACATGAAGGAACGTTTCTA

Downstream 100 bases:

>100_bases
ATATCCCCGCCCTTTGCAACGCAAGGGGCGTATTCTACTAACTACAAGGATAGGTTATGTCTTCATCACCAACAGTTTCA
TGGCGTGAAACTGTACAAAG

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLKVICASLVLTSSLALAGPKVEFETSLGNFTVELNEEKAPISVENFVKYVKDGSYVGSQFHRVIPGFMAQGGGFDEKMN
RLPSYAPIKNEASNGLKNVTGSIAMARTQDPNSATRQFFINLVDNEFLDYDLQPPGYAVFGQVTEGFDVIQQMASKPTKS
QGYMRDIPVEPIVITKATLLP

Sequences:

>Translated_181_residues
MLKVICASLVLTSSLALAGPKVEFETSLGNFTVELNEEKAPISVENFVKYVKDGSYVGSQFHRVIPGFMAQGGGFDEKMN
RLPSYAPIKNEASNGLKNVTGSIAMARTQDPNSATRQFFINLVDNEFLDYDLQPPGYAVFGQVTEGFDVIQQMASKPTKS
QGYMRDIPVEPIVITKATLLP
>Mature_181_residues
MLKVICASLVLTSSLALAGPKVEFETSLGNFTVELNEEKAPISVENFVKYVKDGSYVGSQFHRVIPGFMAQGGGFDEKMN
RLPSYAPIKNEASNGLKNVTGSIAMARTQDPNSATRQFFINLVDNEFLDYDLQPPGYAVFGQVTEGFDVIQQMASKPTKS
QGYMRDIPVEPIVITKATLLP

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7706339, Length=160, Percent_Identity=30.625, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI19557636, Length=159, Percent_Identity=31.4465408805031, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI22547215, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI22547212, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI7657473, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI5174637, Length=156, Percent_Identity=31.4102564102564, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI303305009, Length=166, Percent_Identity=30.7228915662651, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789763, Length=182, Percent_Identity=50.5494505494505, Blast_Score=183, Evalue=5e-48,
Organism=Escherichia coli, GI1786736, Length=161, Percent_Identity=47.2049689440994, Blast_Score=158, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17552780, Length=161, Percent_Identity=33.5403726708075, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565860, Length=175, Percent_Identity=33.7142857142857, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323562, Length=184, Percent_Identity=31.5217391304348, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17986117, Length=160, Percent_Identity=30.625, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136360, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 19803; Mature: 19803

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVICASLVLTSSLALAGPKVEFETSLGNFTVELNEEKAPISVENFVKYVKDGSYVGSQ
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHH
FHRVIPGFMAQGGGFDEKMNRLPSYAPIKNEASNGLKNVTGSIAMARTQDPNSATRQFFI
HHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHCCHHHHHCCEEEEECCCCCHHHHHHHH
NLVDNEFLDYDLQPPGYAVFGQVTEGFDVIQQMASKPTKSQGYMRDIPVEPIVITKATLL
HHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECEECC
P
C
>Mature Secondary Structure
MLKVICASLVLTSSLALAGPKVEFETSLGNFTVELNEEKAPISVENFVKYVKDGSYVGSQ
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHH
FHRVIPGFMAQGGGFDEKMNRLPSYAPIKNEASNGLKNVTGSIAMARTQDPNSATRQFFI
HHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHCCHHHHHCCEEEEECCCCCHHHHHHHH
NLVDNEFLDYDLQPPGYAVFGQVTEGFDVIQQMASKPTKSQGYMRDIPVEPIVITKATLL
HHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECEECC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]