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The map label for this gene is rlmG

Identifier: 37680776

GI number: 37680776

Start: 2620569

End: 2621699

Strand: Direct

Name: rlmG

Synonym: VV2592

Alternate gene names: 37680776

Gene position: 2620569-2621699 (Clockwise)

Preceding gene: 37680766

Following gene: 37680778

Centisome position: 78.12

GC content: 45.0

Gene sequence:

>1131_bases
ATGAAAACCGAACTCAATCTCTTTGACCGACAACTCACCCTATTCAGATACCCAAACAACGCCAATGAAACCCTGCAAGC
ATGGGACGCGGGCGATGAATACTTGATCAATTATGTGGAAGAGTTGGCCTTAGACACCCCACAGAATATTCTTATCCTGA
ACGATCACTTCGGCGCTCTATCATGCTGGTTTTCTGCTCAACACCAAGTCACTATGATGAGTGATTCTTTTATCTCTCAG
CAAGGGGCAAAAGAAAATTTACAACGAAATCAATGCCGTGAAGTAAAGCTACTGACCACGCTAGACGCAATTCCTACAGA
GACCAGTTTGGTGTTATTTCAACTTCCAAAAAACAACCGTCATTTGACTTGGCAGTTAACTCAATTGCGTAAAACGCTCT
CACCAGACGTCCCAGTGGTCGCCGTGAATAAAGCCAAAGAAATCCATACCTCAACCCTCAAACTGTTTGAAAAGTATCTA
GGGACCACAAAAACCTCTCTTGCTTGGAAAAAACATCGTCTCGTTTTTTGCCAAGCAGACTGTGCGCAAATGAATGACAT
CAGCCCAGTGACTCGTTGGAATGTTGAAGAACACAAGATGACACTCAACAACTTGCCAAACGTCTATTCTGGTGAGAGTC
TCGATTTGGGCGCGCGTTTTATGCTCGAGCACATCCCGCAAGATGATAACCTCAAGCACATTATCGATTTAGGCTGTGGT
AACGGGGTGTTATCCGTCAAAGCCGCGCAGCTCAACCCGAAAGCGAAATTCACCTTGGTGGATGAAAGCTACATGGCCAT
TGAGTCCGCTCGCCTCAATTTACAAGAAAATGTCACAGCATCAGTGGATGCTGAGTATATTGCCAATAACTGTTTGGACG
GGTTTGCAGGCGAGATCGCCGATCTGATTTTATGCAACCCCCCTTTCCATCAACAACAAGCGATCACGGATCACATTGCT
TGGCAGATGTTTTGCGACGCCAAACGTGTCCTTAAAAGAGGGGGCAAACTGCAAGTGATCGGCAATCGCCACCTTGGTTA
TGATGGAAAACTCAAGCGCCTTTATGGTGACAAGAATGTGAAATTAGTCGCTTCTAATAGTAAGTTTGTGATTTTACAGG
CAACTAAATAG

Upstream 100 bases:

>100_bases
TAGACGAAGATCCCTGAGTGTTTTTTGCTAGCTGTGCCTATCTTGTTTGCTATCCTTCTGCGAGAATATCGTTAAAACTC
AATGAAACAAACACCATTGT

Downstream 100 bases:

>100_bases
CCTGCTAGTTTTAATTAAAGACAATCTTCACTTTAATATACGTTTACGTACAGCCTCTGCTGCTAGAAAAGGAATATACG
ATGAAAAAATTGGTTTTAGC

Product: 16S RNA G1207 methylase RsmC

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKTELNLFDRQLTLFRYPNNANETLQAWDAGDEYLINYVEELALDTPQNILILNDHFGALSCWFSAQHQVTMMSDSFISQ
QGAKENLQRNQCREVKLLTTLDAIPTETSLVLFQLPKNNRHLTWQLTQLRKTLSPDVPVVAVNKAKEIHTSTLKLFEKYL
GTTKTSLAWKKHRLVFCQADCAQMNDISPVTRWNVEEHKMTLNNLPNVYSGESLDLGARFMLEHIPQDDNLKHIIDLGCG
NGVLSVKAAQLNPKAKFTLVDESYMAIESARLNLQENVTASVDAEYIANNCLDGFAGEIADLILCNPPFHQQQAITDHIA
WQMFCDAKRVLKRGGKLQVIGNRHLGYDGKLKRLYGDKNVKLVASNSKFVILQATK

Sequences:

>Translated_376_residues
MKTELNLFDRQLTLFRYPNNANETLQAWDAGDEYLINYVEELALDTPQNILILNDHFGALSCWFSAQHQVTMMSDSFISQ
QGAKENLQRNQCREVKLLTTLDAIPTETSLVLFQLPKNNRHLTWQLTQLRKTLSPDVPVVAVNKAKEIHTSTLKLFEKYL
GTTKTSLAWKKHRLVFCQADCAQMNDISPVTRWNVEEHKMTLNNLPNVYSGESLDLGARFMLEHIPQDDNLKHIIDLGCG
NGVLSVKAAQLNPKAKFTLVDESYMAIESARLNLQENVTASVDAEYIANNCLDGFAGEIADLILCNPPFHQQQAITDHIA
WQMFCDAKRVLKRGGKLQVIGNRHLGYDGKLKRLYGDKNVKLVASNSKFVILQATK
>Mature_376_residues
MKTELNLFDRQLTLFRYPNNANETLQAWDAGDEYLINYVEELALDTPQNILILNDHFGALSCWFSAQHQVTMMSDSFISQ
QGAKENLQRNQCREVKLLTTLDAIPTETSLVLFQLPKNNRHLTWQLTQLRKTLSPDVPVVAVNKAKEIHTSTLKLFEKYL
GTTKTSLAWKKHRLVFCQADCAQMNDISPVTRWNVEEHKMTLNNLPNVYSGESLDLGARFMLEHIPQDDNLKHIIDLGCG
NGVLSVKAAQLNPKAKFTLVDESYMAIESARLNLQENVTASVDAEYIANNCLDGFAGEIADLILCNPPFHQQQAITDHIA
WQMFCDAKRVLKRGGKLQVIGNRHLGYDGKLKRLYGDKNVKLVASNSKFVILQATK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=372, Percent_Identity=44.8924731182796, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI1790830, Length=279, Percent_Identity=27.9569892473118, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_VIBVU (Q8DBJ8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760698.1
- ProteinModelPortal:   Q8DBJ8
- SMR:   Q8DBJ8
- GeneID:   1178724
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1819
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- ProtClustDB:   CLSK874746
- BioCyc:   VVUL216895:VV1_1819-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 42546; Mature: 42546

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTELNLFDRQLTLFRYPNNANETLQAWDAGDEYLINYVEELALDTPQNILILNDHFGAL
CCCCCCHHCCEEEEEECCCCCCCHHEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEE
SCWFSAQHQVTMMSDSFISQQGAKENLQRNQCREVKLLTTLDAIPTETSLVLFQLPKNNR
EEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHEEEEEEHHCCCCCCCEEEEEEECCCCC
HLTWQLTQLRKTLSPDVPVVAVNKAKEIHTSTLKLFEKYLGTTKTSLAWKKHRLVFCQAD
EEEEEHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHEEEECEEEEEECC
CAQMNDISPVTRWNVEEHKMTLNNLPNVYSGESLDLGARFMLEHIPQDDNLKHIIDLGCG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
NGVLSVKAAQLNPKAKFTLVDESYMAIESARLNLQENVTASVDAEYIANNCLDGFAGEIA
CCEEEEEEEECCCCEEEEEECCHHHHHHHHHCCHHCCCEECCCHHHHHHHHHHHHHHHHH
DLILCNPPFHQQQAITDHIAWQMFCDAKRVLKRGGKLQVIGNRHLGYDGKLKRLYGDKNV
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCE
KLVASNSKFVILQATK
EEEECCCEEEEEEECC
>Mature Secondary Structure
MKTELNLFDRQLTLFRYPNNANETLQAWDAGDEYLINYVEELALDTPQNILILNDHFGAL
CCCCCCHHCCEEEEEECCCCCCCHHEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEE
SCWFSAQHQVTMMSDSFISQQGAKENLQRNQCREVKLLTTLDAIPTETSLVLFQLPKNNR
EEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHEEEEEEHHCCCCCCCEEEEEEECCCCC
HLTWQLTQLRKTLSPDVPVVAVNKAKEIHTSTLKLFEKYLGTTKTSLAWKKHRLVFCQAD
EEEEEHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHEEEECEEEEEECC
CAQMNDISPVTRWNVEEHKMTLNNLPNVYSGESLDLGARFMLEHIPQDDNLKHIIDLGCG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
NGVLSVKAAQLNPKAKFTLVDESYMAIESARLNLQENVTASVDAEYIANNCLDGFAGEIA
CCEEEEEEEECCCCEEEEEECCHHHHHHHHHCCHHCCCEECCCHHHHHHHHHHHHHHHHH
DLILCNPPFHQQQAITDHIAWQMFCDAKRVLKRGGKLQVIGNRHLGYDGKLKRLYGDKNV
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCE
KLVASNSKFVILQATK
EEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA