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The map label for this gene is pleD [H]

Identifier: 37680766

GI number: 37680766

Start: 2611008

End: 2612258

Strand: Direct

Name: pleD [H]

Synonym: VV2582

Alternate gene names: 37680766

Gene position: 2611008-2612258 (Clockwise)

Preceding gene: 37680764

Following gene: 37680776

Centisome position: 77.84

GC content: 44.68

Gene sequence:

>1251_bases
ATGTTAGTTGTTAATAATGGTCCGGAGGCCTCTTCCTTGGGTAATAAAATACTAGTTGTAGAAGATAGCCGAGCCTTTCG
TAATTACCTCCAGCAACAACTCTCCCAAGCGGGATATGACGTTTTTGCGGCAGAGAATTTAGCTGAAGCGCAAACTTTCC
TTGCAGAAAATCACGAGTTTCTTTGCGCCGTTTTGGATTATTGTCTGCCAGACGGTCAAGATGGTGAAGTGATTGATCTT
GCGTTACATCACCAGCAAAAAGTGATCGTCCTAACGGCGATGTTTAGCGAAGAGATTCGCTCTAAAGTGCTTGCCAAAGG
GGTTTTGGATTACATTTTGAAAGACAGTATGGCGTCAGTTTCCTACTTGCTGCCTCTAATTAAACGCCTCACGAACAACC
GACAGCATAAGGCATTGGTGGTTGACGACTCCAAAGTGGTGAGAAAGCACATTACACAGTTACTGGAACACCAATATATC
GAAACACTGGAAGCGGAAAATGGCACTCAAGCGCTAGAGGTCCTGAAGCAACATCCCGAAGTGACGTTTGTGATCACCGA
CCATGACATGCCAGAAAAAGACGGCATCACCATGATTCGTGAAATTCGCGTACATACCGATAAGAACAAGTTAGCCATTT
TAGGACTGTCTGGCAGCGACGATCGGACGATGACTGCCCGCTTTTTGAAAGCAGGCGCGAATGACTTTCTCTATAAGCCC
TTTAATCAGGAAGAGTTTTTCTGCCGTGTCCATCAAATACTCGACATGAAAGAAGCAACCAATGAGCTCTATCGTATGGC
TAACCAAGATGCCCTGACAGGCCTTTGGAATCGTCGTTATCTTTTTAGTCAGTCCTGTAACAGCTGTGAATTGCGCCATA
TTGCGATGCTTGATATTGATCACTTCAAAAAAGTGAATGATACCTATGGCCATGATGGCGGTGACATCGCCTTAAAAACG
GTCGCTAACATCTTGAAAATTTATTTCCCCGGAGAAATTGTCGCGCGGTTTGGCGGTGAAGAATTTTGTATTCAATCTTG
CCAAAGTTACGACGACTTTGTCACTCGCTTAGAGCACATGAGGCAGCGGGTCGAGAAAACCACCATTACACACAAAGCGC
ATAACATTCATATCACCATCAGCATTGGTGTGGCTTCTTTGCAAGATGAGCTTGAGAATCAAATCAAAGAAGCCGATGGC
TACTTGTATCAAGCAAAAGCCAACGGACGAAACCAAGTAGTTAGCCGATAA

Upstream 100 bases:

>100_bases
TAATGAATCCGTATGCATGTCGTTATTTTGAGATTCAGCCCAATTAGCAAACAAACAAATCGCTTATTTGTGGCAACTTT
GTTAGTCCTACATATTATTT

Downstream 100 bases:

>100_bases
AGAAAACGCCCGATGCGGGCGTTTTTCATTTTAAGCGATGCGGTCTTTGTCCCACTGAGCCAATTTTTCCGCTCGCGCGG
CTTCACGTGCTTCGCGTTTT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLVVNNGPEASSLGNKILVVEDSRAFRNYLQQQLSQAGYDVFAAENLAEAQTFLAENHEFLCAVLDYCLPDGQDGEVIDL
ALHHQQKVIVLTAMFSEEIRSKVLAKGVLDYILKDSMASVSYLLPLIKRLTNNRQHKALVVDDSKVVRKHITQLLEHQYI
ETLEAENGTQALEVLKQHPEVTFVITDHDMPEKDGITMIREIRVHTDKNKLAILGLSGSDDRTMTARFLKAGANDFLYKP
FNQEEFFCRVHQILDMKEATNELYRMANQDALTGLWNRRYLFSQSCNSCELRHIAMLDIDHFKKVNDTYGHDGGDIALKT
VANILKIYFPGEIVARFGGEEFCIQSCQSYDDFVTRLEHMRQRVEKTTITHKAHNIHITISIGVASLQDELENQIKEADG
YLYQAKANGRNQVVSR

Sequences:

>Translated_416_residues
MLVVNNGPEASSLGNKILVVEDSRAFRNYLQQQLSQAGYDVFAAENLAEAQTFLAENHEFLCAVLDYCLPDGQDGEVIDL
ALHHQQKVIVLTAMFSEEIRSKVLAKGVLDYILKDSMASVSYLLPLIKRLTNNRQHKALVVDDSKVVRKHITQLLEHQYI
ETLEAENGTQALEVLKQHPEVTFVITDHDMPEKDGITMIREIRVHTDKNKLAILGLSGSDDRTMTARFLKAGANDFLYKP
FNQEEFFCRVHQILDMKEATNELYRMANQDALTGLWNRRYLFSQSCNSCELRHIAMLDIDHFKKVNDTYGHDGGDIALKT
VANILKIYFPGEIVARFGGEEFCIQSCQSYDDFVTRLEHMRQRVEKTTITHKAHNIHITISIGVASLQDELENQIKEADG
YLYQAKANGRNQVVSR
>Mature_416_residues
MLVVNNGPEASSLGNKILVVEDSRAFRNYLQQQLSQAGYDVFAAENLAEAQTFLAENHEFLCAVLDYCLPDGQDGEVIDL
ALHHQQKVIVLTAMFSEEIRSKVLAKGVLDYILKDSMASVSYLLPLIKRLTNNRQHKALVVDDSKVVRKHITQLLEHQYI
ETLEAENGTQALEVLKQHPEVTFVITDHDMPEKDGITMIREIRVHTDKNKLAILGLSGSDDRTMTARFLKAGANDFLYKP
FNQEEFFCRVHQILDMKEATNELYRMANQDALTGLWNRRYLFSQSCNSCELRHIAMLDIDHFKKVNDTYGHDGGDIALKT
VANILKIYFPGEIVARFGGEEFCIQSCQSYDDFVTRLEHMRQRVEKTTITHKAHNIHITISIGVASLQDELENQIKEADG
YLYQAKANGRNQVVSR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=37.7049180327869, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=32.3529411764706, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=33.1550802139037, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=38.8535031847134, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=34.3558282208589, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=30.0518134715026, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=30.0613496932515, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=165, Percent_Identity=29.0909090909091, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=31.7365269461078, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=29.3103448275862, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=31.5436241610738, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=32.0987654320988, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47320; Mature: 47320

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVNNGPEASSLGNKILVVEDSRAFRNYLQQQLSQAGYDVFAAENLAEAQTFLAENHEF
CEEECCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHH
LCAVLDYCLPDGQDGEVIDLALHHQQKVIVLTAMFSEEIRSKVLAKGVLDYILKDSMASV
HHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLLPLIKRLTNNRQHKALVVDDSKVVRKHITQLLEHQYIETLEAENGTQALEVLKQHPE
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
VTFVITDHDMPEKDGITMIREIRVHTDKNKLAILGLSGSDDRTMTARFLKAGANDFLYKP
EEEEEECCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCC
FNQEEFFCRVHQILDMKEATNELYRMANQDALTGLWNRRYLFSQSCNSCELRHIAMLDID
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCCEEEEEEEEHH
HFKKVNDTYGHDGGDIALKTVANILKIYFPGEIVARFGGEEFCIQSCQSYDDFVTRLEHM
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RQRVEKTTITHKAHNIHITISIGVASLQDELENQIKEADGYLYQAKANGRNQVVSR
HHHHHHHHHEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLVVNNGPEASSLGNKILVVEDSRAFRNYLQQQLSQAGYDVFAAENLAEAQTFLAENHEF
CEEECCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHH
LCAVLDYCLPDGQDGEVIDLALHHQQKVIVLTAMFSEEIRSKVLAKGVLDYILKDSMASV
HHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLLPLIKRLTNNRQHKALVVDDSKVVRKHITQLLEHQYIETLEAENGTQALEVLKQHPE
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
VTFVITDHDMPEKDGITMIREIRVHTDKNKLAILGLSGSDDRTMTARFLKAGANDFLYKP
EEEEEECCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCC
FNQEEFFCRVHQILDMKEATNELYRMANQDALTGLWNRRYLFSQSCNSCELRHIAMLDID
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCCEEEEEEEEHH
HFKKVNDTYGHDGGDIALKTVANILKIYFPGEIVARFGGEEFCIQSCQSYDDFVTRLEHM
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RQRVEKTTITHKAHNIHITISIGVASLQDELENQIKEADGYLYQAKANGRNQVVSR
HHHHHHHHHEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA