Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 37680717

GI number: 37680717

Start: 2562117

End: 2563760

Strand: Direct

Name: mltD [H]

Synonym: VV2533

Alternate gene names: 37680717

Gene position: 2562117-2563760 (Clockwise)

Preceding gene: 37680716

Following gene: 37680718

Centisome position: 76.38

GC content: 48.72

Gene sequence:

>1644_bases
TTGTCTCCTGACCAAGGAGGCAAGTATTATCAGCAGCCCAAAGTTAAACAGGCTGGAACCATGCGTTTTAAGTTTAGTTG
GGTGTTTGCACTACTATTGCTTTCTGGCTGTCAGAGCCTACAAACAGAGAGCAATACCAGTGCCGCGCCTGAGACCAATG
CCTCGCAAGCAAAATCGTCGCAGCAAACTGCGAAAAAAGTAAAACCAAGTGACAAAACGCTTGAGAACGTTGCCCCTGAG
CAACCTGTCGTCACACCTCAAACTCAGGAAGACGTTTGGCAACGCATCGCGATGCAGCTTTCCTTAGACGTGCCTGACCA
CGAGAAGGTCGATTATTACCGAACTTGGTACCTCAAACATCCTAATCACCTTAAAACCGTCTCACAACGTGCGACCCCAT
TTCTCTATCTGATCACTGAGAAAATTGAAGCACGTGATTTGCCTCTTGAGCTGGCATTGTTACCTGTCGTAGAAAGCTCA
TTCGACCCATTTGCCTACTCTCATGGCAGTGCCGCTGGCTTATGGCAGTTTGTTCCGGGCACTGGGAAAATGTATGGTCT
AGAACAAAATTTTTGGTATGACGGTCGCCGAGACGTGGCAGCCGCCACCGACGCTGCGCTCGATTATCTTACCTACCTCA
ACAAACGTTTTGATGGTGACTGGAATCACGCGATCGCGGCGTACAACAGCGGTGGTGGCCGTGTATCGAGCGCGATCCGT
AAGAACAACAAACTGGGTAAACCTATCGATTTCTTCTCACTAGATTTGCCCAAAGAAACCAGTGGCTATGTGCCAAAACT
GCTCGCCCTTGCCGATATTGTCGCCAATCAAGAGAAATACGGCATTGAGATCCCTGCGATTCCAAACAAACCCGTGTTGG
CACTGATTGATCCTAAGGAGCAATTGGATCTCGCCATTGCTGCACAATACGCCGGGCTGAATGTCAAAGAGCTGCAAAGC
TATAATCCGGCCTACAACCAGTGGTCTACGGCACCGGATGGTCCTTACCAACTGCTAATTCCTGTTGATAAAGCAGAGCA
GTTCATCGCGAAAGTAGAAGAAAATCGCGGCAAAGGCATGAAGATGGTGCGCTACAAAGTGCGCTCTGGCGACACCCTCA
GCGTCTTAGCGGAAAAACATAACACCACCAGTGAAGTGATCCGTACCGCCAATAGCTTGAGCGGCAACAACATTCGTGTC
GGCCAGTACCTACTGATCCCTACATCGCAAAAAGACGCCAGCGCTTACACATTGTCGGCAGCAAACCGCTTGGCTAAGAC
CCAAGCCACTGCTCGCGGTAAATATAAGCTGACGCACACCGTTAAATCCGGCGAAAGCTTATGGAGCATTGCTAAAGCCA
ATAAAGTGGATCACCAAGCATTAGCGAAGTGGAATGGCATGGGACCACGTGACACATTGCGCGTTGGGCAAGATCTGGTG
ATTTGGAAAGAAAGCTCTGAAGGCGCCATTATCCGTACCGTTTTCTATAAAGTACGTTCAGGTGACACCATCAGTGGCAT
CGCGACCAAGTTTAAAGTCAAAAGCAACGACATTGTGAAGTGGAACGCGCTACAAAATCAAAAGTACCTGAAACCAGGTC
AACAATTAAAACTCTACGTGGATGTAACTAAGGTAAGTGTATGA

Upstream 100 bases:

>100_bases
GTGTTATTCACTCGGTTTCTAGCAGAACGAAAAATAGCGATCCACTCTCGGTTTTTACAGCATTACGTGAGTGGAAGAAC
GAATTTTAACTTTTTGATGC

Downstream 100 bases:

>100_bases
CCCCTTCCAGTAACCCGTTTGTCATGATGCTTGACATATTTCGCTCTCCAACCTCATGTTTTCTCGCGCTTTATCAGCGT
GGTGCTTGGGGCTGGCAACC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSPDQGGKYYQQPKVKQAGTMRFKFSWVFALLLLSGCQSLQTESNTSAAPETNASQAKSSQQTAKKVKPSDKTLENVAPE
QPVVTPQTQEDVWQRIAMQLSLDVPDHEKVDYYRTWYLKHPNHLKTVSQRATPFLYLITEKIEARDLPLELALLPVVESS
FDPFAYSHGSAAGLWQFVPGTGKMYGLEQNFWYDGRRDVAAATDAALDYLTYLNKRFDGDWNHAIAAYNSGGGRVSSAIR
KNNKLGKPIDFFSLDLPKETSGYVPKLLALADIVANQEKYGIEIPAIPNKPVLALIDPKEQLDLAIAAQYAGLNVKELQS
YNPAYNQWSTAPDGPYQLLIPVDKAEQFIAKVEENRGKGMKMVRYKVRSGDTLSVLAEKHNTTSEVIRTANSLSGNNIRV
GQYLLIPTSQKDASAYTLSAANRLAKTQATARGKYKLTHTVKSGESLWSIAKANKVDHQALAKWNGMGPRDTLRVGQDLV
IWKESSEGAIIRTVFYKVRSGDTISGIATKFKVKSNDIVKWNALQNQKYLKPGQQLKLYVDVTKVSV

Sequences:

>Translated_547_residues
MSPDQGGKYYQQPKVKQAGTMRFKFSWVFALLLLSGCQSLQTESNTSAAPETNASQAKSSQQTAKKVKPSDKTLENVAPE
QPVVTPQTQEDVWQRIAMQLSLDVPDHEKVDYYRTWYLKHPNHLKTVSQRATPFLYLITEKIEARDLPLELALLPVVESS
FDPFAYSHGSAAGLWQFVPGTGKMYGLEQNFWYDGRRDVAAATDAALDYLTYLNKRFDGDWNHAIAAYNSGGGRVSSAIR
KNNKLGKPIDFFSLDLPKETSGYVPKLLALADIVANQEKYGIEIPAIPNKPVLALIDPKEQLDLAIAAQYAGLNVKELQS
YNPAYNQWSTAPDGPYQLLIPVDKAEQFIAKVEENRGKGMKMVRYKVRSGDTLSVLAEKHNTTSEVIRTANSLSGNNIRV
GQYLLIPTSQKDASAYTLSAANRLAKTQATARGKYKLTHTVKSGESLWSIAKANKVDHQALAKWNGMGPRDTLRVGQDLV
IWKESSEGAIIRTVFYKVRSGDTISGIATKFKVKSNDIVKWNALQNQKYLKPGQQLKLYVDVTKVSV
>Mature_546_residues
SPDQGGKYYQQPKVKQAGTMRFKFSWVFALLLLSGCQSLQTESNTSAAPETNASQAKSSQQTAKKVKPSDKTLENVAPEQ
PVVTPQTQEDVWQRIAMQLSLDVPDHEKVDYYRTWYLKHPNHLKTVSQRATPFLYLITEKIEARDLPLELALLPVVESSF
DPFAYSHGSAAGLWQFVPGTGKMYGLEQNFWYDGRRDVAAATDAALDYLTYLNKRFDGDWNHAIAAYNSGGGRVSSAIRK
NNKLGKPIDFFSLDLPKETSGYVPKLLALADIVANQEKYGIEIPAIPNKPVLALIDPKEQLDLAIAAQYAGLNVKELQSY
NPAYNQWSTAPDGPYQLLIPVDKAEQFIAKVEENRGKGMKMVRYKVRSGDTLSVLAEKHNTTSEVIRTANSLSGNNIRVG
QYLLIPTSQKDASAYTLSAANRLAKTQATARGKYKLTHTVKSGESLWSIAKANKVDHQALAKWNGMGPRDTLRVGQDLVI
WKESSEGAIIRTVFYKVRSGDTISGIATKFKVKSNDIVKWNALQNQKYLKPGQQLKLYVDVTKVSV

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=452, Percent_Identity=35.8407079646018, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 60844; Mature: 60713

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDQGGKYYQQPKVKQAGTMRFKFSWVFALLLLSGCQSLQTESNTSAAPETNASQAKSS
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QQTAKKVKPSDKTLENVAPEQPVVTPQTQEDVWQRIAMQLSLDVPDHEKVDYYRTWYLKH
HHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCEEEEEEEEC
PNHLKTVSQRATPFLYLITEKIEARDLPLELALLPVVESSFDPFAYSHGSAAGLWQFVPG
CHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEHHHCCCCCCEECCCCCCCEEEECCC
TGKMYGLEQNFWYDGRRDVAAATDAALDYLTYLNKRFDGDWNHAIAAYNSGGGRVSSAIR
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
KNNKLGKPIDFFSLDLPKETSGYVPKLLALADIVANQEKYGIEIPAIPNKPVLALIDPKE
HCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCEECCCCCCCCEEEEECCHH
QLDLAIAAQYAGLNVKELQSYNPAYNQWSTAPDGPYQLLIPVDKAEQFIAKVEENRGKGM
HHCEEEEHHHCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCE
KMVRYKVRSGDTLSVLAEKHNTTSEVIRTANSLSGNNIRVGQYLLIPTSQKDASAYTLSA
EEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCEEEHHH
ANRLAKTQATARGKYKLTHTVKSGESLWSIAKANKVDHQALAKWNGMGPRDTLRVGQDLV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCCCEEE
IWKESSEGAIIRTVFYKVRSGDTISGIATKFKVKSNDIVKWNALQNQKYLKPGQQLKLYV
EEEECCCCCEEEEEEEHHCCCCEEECCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEE
DVTKVSV
EEEEECC
>Mature Secondary Structure 
SPDQGGKYYQQPKVKQAGTMRFKFSWVFALLLLSGCQSLQTESNTSAAPETNASQAKSS
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QQTAKKVKPSDKTLENVAPEQPVVTPQTQEDVWQRIAMQLSLDVPDHEKVDYYRTWYLKH
HHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCEEEEEEEEC
PNHLKTVSQRATPFLYLITEKIEARDLPLELALLPVVESSFDPFAYSHGSAAGLWQFVPG
CHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEHHHCCCCCCEECCCCCCCEEEECCC
TGKMYGLEQNFWYDGRRDVAAATDAALDYLTYLNKRFDGDWNHAIAAYNSGGGRVSSAIR
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
KNNKLGKPIDFFSLDLPKETSGYVPKLLALADIVANQEKYGIEIPAIPNKPVLALIDPKE
HCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCEECCCCCCCCEEEEECCHH
QLDLAIAAQYAGLNVKELQSYNPAYNQWSTAPDGPYQLLIPVDKAEQFIAKVEENRGKGM
HHCEEEEHHHCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCE
KMVRYKVRSGDTLSVLAEKHNTTSEVIRTANSLSGNNIRVGQYLLIPTSQKDASAYTLSA
EEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCEEEHHH
ANRLAKTQATARGKYKLTHTVKSGESLWSIAKANKVDHQALAKWNGMGPRDTLRVGQDLV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCCCEEE
IWKESSEGAIIRTVFYKVRSGDTISGIATKFKVKSNDIVKWNALQNQKYLKPGQQLKLYV
EEEECCCCCEEEEEEEHHCCCCEEECCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEE
DVTKVSV
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]