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The map label for this gene is ycdT [H]

Identifier: 37680252

GI number: 37680252

Start: 2072488

End: 2073807

Strand: Direct

Name: ycdT [H]

Synonym: VV2068

Alternate gene names: 37680252

Gene position: 2072488-2073807 (Clockwise)

Preceding gene: 37680251

Following gene: 37680264

Centisome position: 61.78

GC content: 41.67

Gene sequence:

>1320_bases
ATGGTCCTCTATCAATCAAAATTGTCGCCATCAACAGCAACCATAGAATTACGCAGCTTACTGATTATGATTGTGGTTAT
TTTTGTTTTTAATACACCACAAAACCTGATTAAAAGGGACAAAATAGAGAACGTACGATTACAACTGAGTGCATCATTAG
AATCTTTAAATACACGAAAGGAGTTAATACAAAGCTATCTTTCTTTAGCAGACAGTCAAATTGCTCAGCGTTATTTTTCT
GACTCAACCGACTTTATTGATCTGGTCCAAAACTTAATTCAGCATCAAAAAATCATTCGCCGAATTCGGATTATCGATAA
AAACCCAACTGAGCAAGAGACCTATTCTAAGCGTATTATCAGTTTCAATCGCTTTTACCAAAATGACTTAAACCGCCGCC
AGAGACAGACTATTTTGGATATCGAAAATGCTCTGTTTGTCGAGTTCTCACCCATTTATCAACATAACCGTTTAATGGGC
TATTTGACTGTTGAAGTCGACCTGATTCACTTTACCCCTTTGTTTCGCGACAACATGCTCCATGTTGATCTTGATGGTTT
TGTCTATTCCAGTTCGTATGCTGATATCACCGCCTTTACTTACCTCAAACATCGCGAGCAAACCTTACTTCAAGAGCTGA
ATCGTACTCACAAAACATCCGGCGTATTGGACTTTCAAGGAAAAACCTTCGTTTATCAAAACGTAGGACAACTCAATGGC
AAAACGAGTTATTTGGTCAAAATCATTGCCAACGAAGAACTCATCCCTAAATATTTCTACTTAATTCCACTGCTGCTTGC
CATCACGGTAGGCGCTTGTTATTACCTGTACAAGCTGAATAAGGCACAAAAGAAGCTAAAAGAAATCAGCTATTTAGACC
CACTGTCCGGGTTAAACAATCGACACTTTCTTGCTGAAGTCGAAAAACAACAGCTTCCGCTTGAACATTATTATGCCGTT
ATGCTTGATATTGACCACTTTAAGAGTGTCAACGACCGATATGGGCATGACATCGGCGATCAGGTCATTCGCCGTGTGGC
AAAGGTGGTGAAAAGTCGTATCCGCGTCAGTGATTACGCCTTTCGTATTGGTGGTGAAGAATTTCTGTTGCTGGTCAAAA
CCCCTTCGTCCAATGAAGCACGGCAGGTTTGTGAACGCATTCGCCAAGATGTTGAGAACATGACTCAAGCGCCGCACGTC
ACGGTATCCATTGGCTTTACTGCTTTACAGACACAGCTCGACGAGACCATTCGCATCGCCGATTCACATCTGTATGACGC
TAAGCGCAACGGGCGAAATCGTGTTTGCCCTAACGCTTAG

Upstream 100 bases:

>100_bases
TAGCGAGTGCAACAGGTAGACAGAAAGTAACTGATAAATATATCTGGAATCAATTTGCACAACCGAGGATGGGTATGTCG
GTGTTTGAGAAATAGTCAGC

Downstream 100 bases:

>100_bases
CGCCCAGCTCAACTCTCAGTCACTCATGCCTTCCACACCGCCATCTTTGACTCAAGGATGCAGATGTTTCAGCACGCCCG
CTTGATCCAGTTCATCATGC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKIIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVEKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRIADSHLYDAKRNGRNRVCPNA

Sequences:

>Translated_439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKIIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVEKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRIADSHLYDAKRNGRNRVCPNA
>Mature_439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKIIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVEKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRIADSHLYDAKRNGRNRVCPNA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=37.9310344827586, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=36.875, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=35.9756097560976, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=40.1273885350318, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.3609467455621, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=31.8918918918919, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=32.2147651006711, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=150, Percent_Identity=34.6666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=30.7228915662651, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=150, Percent_Identity=30.6666666666667, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=28.7425149700599, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51116; Mature: 51116

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRK
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHEEEEHHHHHHHHHH
ELIQSYLSLADSQIAQRYFSDSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMGYLTVEVDLIHFTPLFRDNML
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEEEEEEEEEEEECHHHHCCEE
HVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
EEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCC
KTSYLVKIIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNN
CHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RHFLAEVEKQQLPLEHYYAVMLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYA
CHHHHHHHHHHCCHHHHHHHEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHVTVSIGFTALQTQLDETIRIA
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHH
DSHLYDAKRNGRNRVCPNA
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRK
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHEEEEHHHHHHHHHH
ELIQSYLSLADSQIAQRYFSDSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMGYLTVEVDLIHFTPLFRDNML
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEEEEEEEEEEEECHHHHCCEE
HVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
EEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCC
KTSYLVKIIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNN
CHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RHFLAEVEKQQLPLEHYYAVMLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYA
CHHHHHHHHHHCCHHHHHHHEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHVTVSIGFTALQTQLDETIRIA
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHH
DSHLYDAKRNGRNRVCPNA
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]