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The map label for this gene is purT

Identifier: 37680147

GI number: 37680147

Start: 1954496

End: 1955671

Strand: Direct

Name: purT

Synonym: VV1963

Alternate gene names: 37680147

Gene position: 1954496-1955671 (Clockwise)

Preceding gene: 161486621

Following gene: 37680149

Centisome position: 58.26

GC content: 48.64

Gene sequence:

>1176_bases
ATGTTCGGTACCGCGACTCGCGAAAGCGCCACACGCGTATTACTTTTGGGCTCTGGTGAACTCGGTAAAGAGGTGGCGAT
CGAATGCCAACGTCTTGGTCTGGAAGTGATTGCCTGTGATCGCTACCCTGATGCACCCGCAATGCAAGTAGCACACCGCA
GTTATGTTTTCGACATGTTAGATGCAAGCGAGCTAGAAAAAGTTATCGCCGCAGAACAACCTGCGTTTGTGGTTCCGGAG
ATCGAGGCCATCGCAACAGACAAGTTGGTAGAACTTGAGGAACAAGGCTTAAACGTGGTGCCTAGTGCAAAAGCAACCAA
ACTCACAATGAACCGTGAAGGGATTCGTCGTCTAGCCGCAGAAGAGCTTGGCCTCACTACCTCACCATACCGCTTTGCAG
ACAACTATCAGCAATTTGTCGAAGCCGTTGAAGCCGTGTCGATTCCTTGCGTCGTAAAACCCGTGATGAGCTCTTCTGGC
AAAGGACAAAGTGTCATTAAATCTCCAGCCGATATTGAGAAAGCATGGCAATATGCTCAAGAAGGTGGTCGCACTGGCGC
TGGACGAGTGATTGTCGAAGGCTTTATCGACTTCGATTATGAGATCACTCTACTCACCGTTCGCGCAGTTGATGGCGTGC
ATTTCTGTGCTCCTATTGGCCATCGACAAGAAGATGGGGATTATCGTGAATCTTGGCAACCACAGCAGATGTCTGAAAAT
GCCATCAAAGCTGCCGAATACACTGCAGAACAGGTCGTCAATGCGTTAGGTGGCTACGGTATTTTCGGTGTAGAACTTTT
CGTAAAAGGTGACAAGGTGATCTTCAATGAAGTCTCCCCTCGCCCACACGATACCGGGCTAGTAACACTGATTTCACAAG
AAATGTCTGAATTTGCTCTCCACGTGCGTGCATTTACTGGCATGCCAGTGAACAAGATTGTTCAATACGGCCCTTCCGCT
TCAGCGGTGATTTTGGGCAATGGTCAATCAGAGAACCTTCGCTTTGACGGAATGTCTGACGCGCTTGAACAACCGCAAAC
CCAGTTGCGTCTATTTGGCAAACCCGATATTAATGGCCGTCGTCGTTTAGGTGTTGTGTTGACACGTCGTTCTAGCACTG
AGAAAGCGGTGGATGCAGCAATAGAAAGTGCTAAGAAAATCAAAATCATTTACTGA

Upstream 100 bases:

>100_bases
CTTTCTTTAAAATCACAAAGCAAACGTTTGCTTTGCAGATTAAATTGAGTATGATCGGCGGCAATTTTATTCTCTCTGAT
TACTTTTTAAAGGAAGCACT

Downstream 100 bases:

>100_bases
ACATCGTTTTTCGATTCAGCCGATTAAAAAGGGTAGCTCACGCTACCCTTTGTTATTTATCGTTTTCAATAACGTAGACT
TCTTCGCTAAAGCGGCTTAA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFGTATRESATRVLLLGSGELGKEVAIECQRLGLEVIACDRYPDAPAMQVAHRSYVFDMLDASELEKVIAAEQPAFVVPE
IEAIATDKLVELEEQGLNVVPSAKATKLTMNREGIRRLAAEELGLTTSPYRFADNYQQFVEAVEAVSIPCVVKPVMSSSG
KGQSVIKSPADIEKAWQYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQQMSEN
AIKAAEYTAEQVVNALGGYGIFGVELFVKGDKVIFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPVNKIVQYGPSA
SAVILGNGQSENLRFDGMSDALEQPQTQLRLFGKPDINGRRRLGVVLTRRSSTEKAVDAAIESAKKIKIIY

Sequences:

>Translated_391_residues
MFGTATRESATRVLLLGSGELGKEVAIECQRLGLEVIACDRYPDAPAMQVAHRSYVFDMLDASELEKVIAAEQPAFVVPE
IEAIATDKLVELEEQGLNVVPSAKATKLTMNREGIRRLAAEELGLTTSPYRFADNYQQFVEAVEAVSIPCVVKPVMSSSG
KGQSVIKSPADIEKAWQYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQQMSEN
AIKAAEYTAEQVVNALGGYGIFGVELFVKGDKVIFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPVNKIVQYGPSA
SAVILGNGQSENLRFDGMSDALEQPQTQLRLFGKPDINGRRRLGVVLTRRSSTEKAVDAAIESAKKIKIIY
>Mature_391_residues
MFGTATRESATRVLLLGSGELGKEVAIECQRLGLEVIACDRYPDAPAMQVAHRSYVFDMLDASELEKVIAAEQPAFVVPE
IEAIATDKLVELEEQGLNVVPSAKATKLTMNREGIRRLAAEELGLTTSPYRFADNYQQFVEAVEAVSIPCVVKPVMSSSG
KGQSVIKSPADIEKAWQYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQQMSEN
AIKAAEYTAEQVVNALGGYGIFGVELFVKGDKVIFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPVNKIVQYGPSA
SAVILGNGQSENLRFDGMSDALEQPQTQLRLFGKPDINGRRRLGVVLTRRSSTEKAVDAAIESAKKIKIIY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=67.6092544987146, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1786733, Length=376, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=356, Percent_Identity=23.5955056179775, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_VIBVU (Q8DA32)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761225.1
- HSSP:   P33221
- ProteinModelPortal:   Q8DA32
- SMR:   Q8DA32
- GeneID:   1179253
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2378
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   VVUL216895:VV1_2378-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42761; Mature: 42761

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 80-80 BINDING 112-112 BINDING 153-153 BINDING 201-201 BINDING 265-265 BINDING 277-277 BINDING 284-284 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTATRESATRVLLLGSGELGKEVAIECQRLGLEVIACDRYPDAPAMQVAHRSYVFDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DASELEKVIAAEQPAFVVPEIEAIATDKLVELEEQGLNVVPSAKATKLTMNREGIRRLAA
CHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHH
EELGLTTSPYRFADNYQQFVEAVEAVSIPCVVKPVMSSSGKGQSVIKSPADIEKAWQYAQ
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHCCCHHHHHHHHHHH
EGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQQMSEN
HCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCHHHHHH
AIKAAEYTAEQVVNALGGYGIFGVELFVKGDKVIFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPVNKIVQYGPSASAVILGNGQSENLRFDGMSDALEQPQTQLRLFGKPDINGR
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCCHHHHHCCCHHEEEECCCCCCCC
RRLGVVLTRRSSTEKAVDAAIESAKKIKIIY
EEEEEEEEECCCHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MFGTATRESATRVLLLGSGELGKEVAIECQRLGLEVIACDRYPDAPAMQVAHRSYVFDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DASELEKVIAAEQPAFVVPEIEAIATDKLVELEEQGLNVVPSAKATKLTMNREGIRRLAA
CHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHH
EELGLTTSPYRFADNYQQFVEAVEAVSIPCVVKPVMSSSGKGQSVIKSPADIEKAWQYAQ
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHCCCHHHHHHHHHHH
EGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQQMSEN
HCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCHHHHHH
AIKAAEYTAEQVVNALGGYGIFGVELFVKGDKVIFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPVNKIVQYGPSASAVILGNGQSENLRFDGMSDALEQPQTQLRLFGKPDINGR
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCCHHHHHCCCHHEEEECCCCCCCC
RRLGVVLTRRSSTEKAVDAAIESAKKIKIIY
EEEEEEEEECCCHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA