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The map label for this gene is hcaT [H]

Identifier: 34498241

GI number: 34498241

Start: 3020264

End: 3021424

Strand: Reverse

Name: hcaT [H]

Synonym: CV_2786

Alternate gene names: 34498241

Gene position: 3021424-3020264 (Counterclockwise)

Preceding gene: 34498242

Following gene: 34498239

Centisome position: 63.59

GC content: 67.36

Gene sequence:

>1161_bases
ATGCAGTCGCTGTTGCCGTTCGCTTCGTTCTACTTCACCTATTTCACCTTCCAGGGCATGTTCAGCCCGTTCTGGGGCCT
GTACCTGCAGTCGCTGTCGTTCAGCGCCTGGCAGATCTCGGTGCTGATGGCGCTGTCCACGCTGGCGCGCATCGTCGCGC
CGGGTTTCTGGGGCTGGCTGGCCGACCGCAGTGGCCGGCGCCGCAACATCATCGTCACCACCTCCATCCTGTCCGCCGCC
AGCTTCTGCCTGGTAGGACTGCACAATGGCTTCTGGTGGATCTTCGTCAGCCTGGCGGTGTCGCATTTCTTCTGGGCCGC
CGCCTTGCCCTTGGTGGAGGCCAGCACCGCCTACCTGACGCGCGACAATCCCGGCCGCTACAGCCGGGTGCGGGTATGGG
GATCGATAGGCTTCGTCTGCCTGGCGATGACGGGCGGCTATCTGCTGGACTGGCTGGGCATCGCGGCGACGCCGTGGATC
GTTTCGGCGCTGTTGGCCGGCGTGGCGCTGGCCGCGTTCAAGGTGCCGGAGGTGGTGCCGCAGGGCAAGCCCAAGCCGGC
CGGGCCGATCTGGGACACGCTGAAGAAGCCGGCGGTGATGGCGCTGTTCGCCTGTTGTTTCCTGCAGGCCTTCGCGCATG
GTCCGTATTACACCTTCTATTCGCTGGGCCTGAAGGCTTTCGGCTACGACAAGGCGGCGGTCGGCGTGCTATGGGCGCTG
GGGGTGGTGTTCGAGGTGGGCGTCTTCATGCTGATGCCGCGCATCATGCGCCGCTTCGGGTTGGAGGCGCTGATGCTGGC
CTCCCTGCTGGCGGCGGTGCTGCGCTTCGGCCTGATTGCCGCCTGCCTGGCCTCGCCGGCGGTGGCGGCCTTCGCCCAGG
CCCTGCACGCGCTCACTTATGCCTTGCACCACGCGTCGGCCATCGGCCTGATCAACCGCATGTTCGCCGAGCAGCACCAC
GGCCGCGCGCAGGGCTTGTACATCGTGGCGTCCTTCGGCGTCGGCGGCAGCGGCGGCGGGTTGATCGGCGGGCTGGTGTG
GCCGCATGGAGGTGTGCTGCCGACTTTCGCGATGTCGGCGGCGGCGGCGCTGATCGGCGTGCTGGTCTGCCTGCGCTGGC
TGAAGCCTGCGGGCAGGCTCAGAGCAGCATCACCAGCTTGA

Upstream 100 bases:

>100_bases
AATTTCCCGCTTCAAATCGTCGTTTTAGCCGTTGCCGGGCGGCGCCGGTCCGGTTCTGGCACCGGGCAGCGCCAGGCGTC
AAACCATCAGAAAGATGAGC

Downstream 100 bases:

>100_bases
ATTCCGGCGCGCTGGCCGGCCGCAGCGTGATCTGCCGCTTCTGCAGGCGGCCGAGCAGCGGATGGTGGAAGCCGCGTTCG
CCGCCTTCGCGCTCCAGCAC

Product: MFS metabolite transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQSLLPFASFYFTYFTFQGMFSPFWGLYLQSLSFSAWQISVLMALSTLARIVAPGFWGWLADRSGRRRNIIVTTSILSAA
SFCLVGLHNGFWWIFVSLAVSHFFWAAALPLVEASTAYLTRDNPGRYSRVRVWGSIGFVCLAMTGGYLLDWLGIAATPWI
VSALLAGVALAAFKVPEVVPQGKPKPAGPIWDTLKKPAVMALFACCFLQAFAHGPYYTFYSLGLKAFGYDKAAVGVLWAL
GVVFEVGVFMLMPRIMRRFGLEALMLASLLAAVLRFGLIAACLASPAVAAFAQALHALTYALHHASAIGLINRMFAEQHH
GRAQGLYIVASFGVGGSGGGLIGGLVWPHGGVLPTFAMSAAAALIGVLVCLRWLKPAGRLRAASPA

Sequences:

>Translated_386_residues
MQSLLPFASFYFTYFTFQGMFSPFWGLYLQSLSFSAWQISVLMALSTLARIVAPGFWGWLADRSGRRRNIIVTTSILSAA
SFCLVGLHNGFWWIFVSLAVSHFFWAAALPLVEASTAYLTRDNPGRYSRVRVWGSIGFVCLAMTGGYLLDWLGIAATPWI
VSALLAGVALAAFKVPEVVPQGKPKPAGPIWDTLKKPAVMALFACCFLQAFAHGPYYTFYSLGLKAFGYDKAAVGVLWAL
GVVFEVGVFMLMPRIMRRFGLEALMLASLLAAVLRFGLIAACLASPAVAAFAQALHALTYALHHASAIGLINRMFAEQHH
GRAQGLYIVASFGVGGSGGGLIGGLVWPHGGVLPTFAMSAAAALIGVLVCLRWLKPAGRLRAASPA
>Mature_386_residues
MQSLLPFASFYFTYFTFQGMFSPFWGLYLQSLSFSAWQISVLMALSTLARIVAPGFWGWLADRSGRRRNIIVTTSILSAA
SFCLVGLHNGFWWIFVSLAVSHFFWAAALPLVEASTAYLTRDNPGRYSRVRVWGSIGFVCLAMTGGYLLDWLGIAATPWI
VSALLAGVALAAFKVPEVVPQGKPKPAGPIWDTLKKPAVMALFACCFLQAFAHGPYYTFYSLGLKAFGYDKAAVGVLWAL
GVVFEVGVFMLMPRIMRRFGLEALMLASLLAAVLRFGLIAACLASPAVAAFAQALHALTYALHHASAIGLINRMFAEQHH
GRAQGLYIVASFGVGGSGGGLIGGLVWPHGGVLPTFAMSAAAALIGVLVCLRWLKPAGRLRAASPA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=321, Percent_Identity=28.3489096573209, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788415, Length=342, Percent_Identity=23.9766081871345, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 41511; Mature: 41511

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLLPFASFYFTYFTFQGMFSPFWGLYLQSLSFSAWQISVLMALSTLARIVAPGFWGWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ADRSGRRRNIIVTTSILSAASFCLVGLHNGFWWIFVSLAVSHFFWAAALPLVEASTAYLT
HCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
RDNPGRYSRVRVWGSIGFVCLAMTGGYLLDWLGIAATPWIVSALLAGVALAAFKVPEVVP
CCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCC
QGKPKPAGPIWDTLKKPAVMALFACCFLQAFAHGPYYTFYSLGLKAFGYDKAAVGVLWAL
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
GVVFEVGVFMLMPRIMRRFGLEALMLASLLAAVLRFGLIAACLASPAVAAFAQALHALTY
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALHHASAIGLINRMFAEQHHGRAQGLYIVASFGVGGSGGGLIGGLVWPHGGVLPTFAMSA
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHH
AAALIGVLVCLRWLKPAGRLRAASPA
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQSLLPFASFYFTYFTFQGMFSPFWGLYLQSLSFSAWQISVLMALSTLARIVAPGFWGWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ADRSGRRRNIIVTTSILSAASFCLVGLHNGFWWIFVSLAVSHFFWAAALPLVEASTAYLT
HCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
RDNPGRYSRVRVWGSIGFVCLAMTGGYLLDWLGIAATPWIVSALLAGVALAAFKVPEVVP
CCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCC
QGKPKPAGPIWDTLKKPAVMALFACCFLQAFAHGPYYTFYSLGLKAFGYDKAAVGVLWAL
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
GVVFEVGVFMLMPRIMRRFGLEALMLASLLAAVLRFGLIAACLASPAVAAFAQALHALTY
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALHHASAIGLINRMFAEQHHGRAQGLYIVASFGVGGSGGGLIGGLVWPHGGVLPTFAMSA
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHH
AAALIGVLVCLRWLKPAGRLRAASPA
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]