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The map label for this gene is leuC1

Identifier: 34498239

GI number: 34498239

Start: 3017996

End: 3019411

Strand: Reverse

Name: leuC1

Synonym: CV_2784

Alternate gene names: 34498239

Gene position: 3019411-3017996 (Counterclockwise)

Preceding gene: 34498241

Following gene: 34498238

Centisome position: 63.55

GC content: 66.81

Gene sequence:

>1416_bases
ATGACCGCGCAAACCTTGTACGACAAACTCTGGAACAGCCACGTGGTGCGCGAGGAGGCCGACGGCACCGCGCTGCTGTA
CATCGATCGCCACCTGGTGCACGAAGTGACCAGCCCGCAGGCCTTCGAGGGCCTGAAGCTGGCCGGCCGCAGGCTGTGGC
GCGTGGATTCGGTGGTATCCACCGCCGACCACAATACGCCTACCGACCACTGGGACCAGGGCATCCAGGACCCGATCTCG
CGCCAGCAGGTGGAAACGCTGGACGCCAACATCAAGGCCTTCGGCGCGCTGGCTTATTTCCCGTTCAAGGACAAGGGGCA
GGGCATCGTCCACGTGATGGGTCCGGAGCAGGGCGCCACGCTGCCGGGCATGACCGTGGTGTGCGGCGACAGCCACACCT
CCACTCACGGCGCTTTCGGCGCGCTGGCGCATGGCATCGGCACCTCTGAGGTGGAGCACGTGATGGCCACTCAGTGCCTG
GTGGCCAAGAAGTCGAAGAACATGCTGGTGCGGGTGGACGGCCGGCTGGGCGCCGGCATCACCGCCAAGGACGTGGCGCT
GGCCATCATCGGCAAGATCGGCACCGCCGGCGGCACCGGCTACGCGATCGAGTTTGGCGGCGAGGCGATCCGCGGGCTTT
CCATGGAAGGCCGGATGACCTTGTGCAATATGGCGATCGAAGGCGGCGCCCGTTCCGGCATGGTGGCGGTGGACGACAAG
ACCATCGAATATGTCAAAGGACGGCCGTTCGCGCCCAAGGGCGAGCAGTGGAACCAGGCGGTTGCCTACTGGAATACCCT
GCATTCCGATGACGGCGCGCATTTCGACCAAGTGGTGGCGCTGGATGCCGCCGACATCCAGCCGCAGGTCACCTGGGGCA
CGTCGCCGGAAATGGTGGCGGAGGTGGGCGGCAAGGTGCCGAACCCGGCCAATGAAAGCGATCCGGTCAAGAAGGCCGGC
ATCGAGCGCGCGCTGGCCTACATGGGCCTGGAAGCGGATACGCCGATAGAGCAGATCCCGGTGGACGTGGTGTTCATCGG
CTCCTGTACCAACAGCCGCATCGAGGATCTGCGCGAGGCCGCCGCGGTGGCCAAGGGCCGCAGCAAGGCCGGCAACGTCA
AGCAGGTGCTGGTGGTGCCGGGCTCCGGTCTGGTCAAAGCGCAGGCCGAGGCCGAAGGGCTGGACAAGATCTTCGTCGCC
GCCGGTTTCGAGTGGCGCGAGCCGGGGTGTTCGATGTGTTTGGCGATGAACGCCGACAGGCTGTTGCCCGGCGAGCGCTG
CGCCTCGACTTCCAACCGAAATTTCGAAGGCCGGCAGGGCCAGGGCGGACGCACCCACCTGGTCAGTCCTGCGATGGCCG
CCGCCGCCGCGGTGGCGGGCCGCTTTGTCGATGTGCGCCGCTTGGCGGCGGGCTAG

Upstream 100 bases:

>100_bases
GGCTTATCCTGTTGGTTTTTATACCAGTATAACTGCTTATCTTGTAACAGGATCAAAGCTGTTCGAATATGCAGTCAACG
ACGACCCGAGGAACCGCAAC

Downstream 100 bases:

>100_bases
GCTGCGTCGGCGGGGAGCAAAAAACTGCGGCGCGTGTCAGACAGGCATGGAAGGTTGCCCGGCGATTTCGCTAGAATGCC
TGCTTATCTTGTCTGACAGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTAQTLYDKLWNSHVVREEADGTALLYIDRHLVHEVTSPQAFEGLKLAGRRLWRVDSVVSTADHNTPTDHWDQGIQDPIS
RQQVETLDANIKAFGALAYFPFKDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQCL
VAKKSKNMLVRVDGRLGAGITAKDVALAIIGKIGTAGGTGYAIEFGGEAIRGLSMEGRMTLCNMAIEGGARSGMVAVDDK
TIEYVKGRPFAPKGEQWNQAVAYWNTLHSDDGAHFDQVVALDAADIQPQVTWGTSPEMVAEVGGKVPNPANESDPVKKAG
IERALAYMGLEADTPIEQIPVDVVFIGSCTNSRIEDLREAAAVAKGRSKAGNVKQVLVVPGSGLVKAQAEAEGLDKIFVA
AGFEWREPGCSMCLAMNADRLLPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVAGRFVDVRRLAAG

Sequences:

>Translated_471_residues
MTAQTLYDKLWNSHVVREEADGTALLYIDRHLVHEVTSPQAFEGLKLAGRRLWRVDSVVSTADHNTPTDHWDQGIQDPIS
RQQVETLDANIKAFGALAYFPFKDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQCL
VAKKSKNMLVRVDGRLGAGITAKDVALAIIGKIGTAGGTGYAIEFGGEAIRGLSMEGRMTLCNMAIEGGARSGMVAVDDK
TIEYVKGRPFAPKGEQWNQAVAYWNTLHSDDGAHFDQVVALDAADIQPQVTWGTSPEMVAEVGGKVPNPANESDPVKKAG
IERALAYMGLEADTPIEQIPVDVVFIGSCTNSRIEDLREAAAVAKGRSKAGNVKQVLVVPGSGLVKAQAEAEGLDKIFVA
AGFEWREPGCSMCLAMNADRLLPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVAGRFVDVRRLAAG
>Mature_470_residues
TAQTLYDKLWNSHVVREEADGTALLYIDRHLVHEVTSPQAFEGLKLAGRRLWRVDSVVSTADHNTPTDHWDQGIQDPISR
QQVETLDANIKAFGALAYFPFKDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQCLV
AKKSKNMLVRVDGRLGAGITAKDVALAIIGKIGTAGGTGYAIEFGGEAIRGLSMEGRMTLCNMAIEGGARSGMVAVDDKT
IEYVKGRPFAPKGEQWNQAVAYWNTLHSDDGAHFDQVVALDAADIQPQVTWGTSPEMVAEVGGKVPNPANESDPVKKAGI
ERALAYMGLEADTPIEQIPVDVVFIGSCTNSRIEDLREAAAVAKGRSKAGNVKQVLVVPGSGLVKAQAEAEGLDKIFVAA
GFEWREPGCSMCLAMNADRLLPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVAGRFVDVRRLAAG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=370, Percent_Identity=29.7297297297297, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI8659555, Length=438, Percent_Identity=25.7990867579909, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI41352693, Length=421, Percent_Identity=26.8408551068884, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=64.5922746781116, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1787531, Length=377, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081781, Length=354, Percent_Identity=23.728813559322, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=368, Percent_Identity=30.1630434782609, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=30.1630434782609, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=316, Percent_Identity=29.4303797468354, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=471, Percent_Identity=26.3269639065817, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=63.6942675159236, Blast_Score=610, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6323335, Length=371, Percent_Identity=29.1105121293801, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=390, Percent_Identity=27.1794871794872, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=425, Percent_Identity=26.1176470588235, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=486, Percent_Identity=26.1316872427983, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=486, Percent_Identity=26.1316872427983, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=372, Percent_Identity=27.4193548387097, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571643, Length=369, Percent_Identity=27.10027100271, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24645686, Length=439, Percent_Identity=27.5626423690205, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137564, Length=431, Percent_Identity=27.1461716937355, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_CHRVO (Q7NUB6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902454.1
- ProteinModelPortal:   Q7NUB6
- SMR:   Q7NUB6
- GeneID:   2548222
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2784
- NMPDR:   fig|243365.1.peg.2784
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q7NUB6
- ProtClustDB:   PRK05478
- BioCyc:   CVIO243365:CV_2784-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49966; Mature: 49835

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTLYDKLWNSHVVREEADGTALLYIDRHLVHEVTSPQAFEGLKLAGRRLWRVDSVVS
CCHHHHHHHHHCCCCEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TADHNTPTDHWDQGIQDPISRQQVETLDANIKAFGALAYFPFKDKGQGIVHVMGPEQGAT
HCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQCLVAKKSKNMLVRVDGRLGAGI
CCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TAKDVALAIIGKIGTAGGTGYAIEFGGEAIRGLSMEGRMTLCNMAIEGGARSGMVAVDDK
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCEEEECCC
TIEYVKGRPFAPKGEQWNQAVAYWNTLHSDDGAHFDQVVALDAADIQPQVTWGTSPEMVA
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCEEECCCCHHHHH
EVGGKVPNPANESDPVKKAGIERALAYMGLEADTPIEQIPVDVVFIGSCTNSRIEDLREA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHH
AAVAKGRSKAGNVKQVLVVPGSGLVKAQAEAEGLDKIFVAAGFEWREPGCSMCLAMNADR
HHHHCCCCCCCCCEEEEEECCCCCEEECHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LLPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVAGRFVDVRRLAAG
CCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQTLYDKLWNSHVVREEADGTALLYIDRHLVHEVTSPQAFEGLKLAGRRLWRVDSVVS
CHHHHHHHHHCCCCEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TADHNTPTDHWDQGIQDPISRQQVETLDANIKAFGALAYFPFKDKGQGIVHVMGPEQGAT
HCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQCLVAKKSKNMLVRVDGRLGAGI
CCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TAKDVALAIIGKIGTAGGTGYAIEFGGEAIRGLSMEGRMTLCNMAIEGGARSGMVAVDDK
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCEEEECCC
TIEYVKGRPFAPKGEQWNQAVAYWNTLHSDDGAHFDQVVALDAADIQPQVTWGTSPEMVA
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCEEECCCCHHHHH
EVGGKVPNPANESDPVKKAGIERALAYMGLEADTPIEQIPVDVVFIGSCTNSRIEDLREA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHH
AAVAKGRSKAGNVKQVLVVPGSGLVKAQAEAEGLDKIFVAAGFEWREPGCSMCLAMNADR
HHHHCCCCCCCCCEEEEEECCCCCEEECHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LLPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVAGRFVDVRRLAAG
CCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782