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The map label for this gene is bscZ [H]

Identifier: 34498131

GI number: 34498131

Start: 2896544

End: 2897692

Strand: Reverse

Name: bscZ [H]

Synonym: CV_2676

Alternate gene names: 34498131

Gene position: 2897692-2896544 (Counterclockwise)

Preceding gene: 34498132

Following gene: 34498130

Centisome position: 60.99

GC content: 68.41

Gene sequence:

>1149_bases
ATGTCGGCCGTTTCCATCCCGCTGTTCGCGGCGTTGCTGCTGGACGCGCTGCCGGCATTGGCGGCCCCCTGCGATGGCTG
GCCGGACTGGCAGGCGTTCCTGAAGAACTATGTTTCCGGCGACGGCAGGGTGGTGGATCGCAGCCAGGACGCCAGGCCCA
CCACCTCGGAAGGCCAAAGCTACGCCTTGTTCTTCGCATTGGCGGCCAACGACCGGCCGGCGTTCGACCGACTGCTGGCC
TGGACCCGCGACAATCTGGCCGACGGCGATCTGGGCGCGCGCCTGCCGGCCTGGTTGTGGGGGCGGGGGCAGGACGGCGG
ATGGAAGGTATTGGACGACAACAGCGCGTCGGATTCCGACATGTGGCTCGCTTACGACCTGCTGGAGGCTGGGCGGTTGT
GGCGCGAGCCGAGCTATGCGGCGCAGGGCAGGCAGCTGGCGCTCCGGATCCTGAGGGAAGAAACCGCCGACCTGCCCGGA
CTGGGCCTGACGCTGCTGCCCGGCAAGGATGGCTTCTTGTTTTCCGACGGCGGTGCCCGGCTCAATCCCAGTTACCTGCC
GCCGCAGCTGCTGGCCCGTTTCGCCTACGGCTTGCCTGATTCCGACTGGGCGAAGCTGCCGAGCGGCAGCGAACGCGTGC
TGCTGGACGGCGCGCCGGCAGGCTTCGCGCCCGACTGGCTGAGACTTCAGCCGGGCAAGGGGCTGCAGCCGGACGCGGAC
AGCAAGGCGAAGGGCGGCTACAGCGCCATCCGCGTCTACCTGTGGGCGGGCATGCTTGCGCCGTCCGCGCCGCTGCGCGC
CGCGCTGCTGGCCAGGTACAAACCCATGGCGCGCTGGGTGGCGCGGGAGGGCGCGCCTCCCGAACAGGTGGACACCCGCA
ATGGCAAGGCGCAGGGAAGGGCTCCGACGGGGTTTTCCTTCGCCTTGCTGCCCTTCTTGCAGGCACAGGGCGAAACGGAA
GCGCTGCGGGCTCTGCTGCAGAGGCTGCGGGCCATGCCGCGAGACGATCGCGCCGATGCCTATTACGACCAGGTATTGAG
GCTGTTCGGCCTGGGCTGGCTGCAGGGGCGCTTTCGTTTCGAGCGCAATGGCAAGTTGCTGCCCGCATGGGAGGGGAAAT
GTTCCGCATCGCTTCCTGTATCGCGCTGA

Upstream 100 bases:

>100_bases
TGCTGCTTGCCATGCTGACGGCGTTGGCGGTGCTGCTCCTGGCCTTCGTCATCCTGCGCATCCTCCGGATACTGGCGGCG
CGCCGGCTGAGGGAGTCCTG

Downstream 100 bases:

>100_bases
GCCTGCTGCTTGCGCCCGCGCGGGGCGCGGAGACGGCCGATCCCGCGCAGGCATTGCTGGAGCAGGCCAGGCAATGGCAG
TTCCTGGAGCGGCCTCTGGA

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSAVSIPLFAALLLDALPALAAPCDGWPDWQAFLKNYVSGDGRVVDRSQDARPTTSEGQSYALFFALAANDRPAFDRLLA
WTRDNLADGDLGARLPAWLWGRGQDGGWKVLDDNSASDSDMWLAYDLLEAGRLWREPSYAAQGRQLALRILREETADLPG
LGLTLLPGKDGFLFSDGGARLNPSYLPPQLLARFAYGLPDSDWAKLPSGSERVLLDGAPAGFAPDWLRLQPGKGLQPDAD
SKAKGGYSAIRVYLWAGMLAPSAPLRAALLARYKPMARWVAREGAPPEQVDTRNGKAQGRAPTGFSFALLPFLQAQGETE
ALRALLQRLRAMPRDDRADAYYDQVLRLFGLGWLQGRFRFERNGKLLPAWEGKCSASLPVSR

Sequences:

>Translated_382_residues
MSAVSIPLFAALLLDALPALAAPCDGWPDWQAFLKNYVSGDGRVVDRSQDARPTTSEGQSYALFFALAANDRPAFDRLLA
WTRDNLADGDLGARLPAWLWGRGQDGGWKVLDDNSASDSDMWLAYDLLEAGRLWREPSYAAQGRQLALRILREETADLPG
LGLTLLPGKDGFLFSDGGARLNPSYLPPQLLARFAYGLPDSDWAKLPSGSERVLLDGAPAGFAPDWLRLQPGKGLQPDAD
SKAKGGYSAIRVYLWAGMLAPSAPLRAALLARYKPMARWVAREGAPPEQVDTRNGKAQGRAPTGFSFALLPFLQAQGETE
ALRALLQRLRAMPRDDRADAYYDQVLRLFGLGWLQGRFRFERNGKLLPAWEGKCSASLPVSR
>Mature_381_residues
SAVSIPLFAALLLDALPALAAPCDGWPDWQAFLKNYVSGDGRVVDRSQDARPTTSEGQSYALFFALAANDRPAFDRLLAW
TRDNLADGDLGARLPAWLWGRGQDGGWKVLDDNSASDSDMWLAYDLLEAGRLWREPSYAAQGRQLALRILREETADLPGL
GLTLLPGKDGFLFSDGGARLNPSYLPPQLLARFAYGLPDSDWAKLPSGSERVLLDGAPAGFAPDWLRLQPGKGLQPDADS
KAKGGYSAIRVYLWAGMLAPSAPLRAALLARYKPMARWVAREGAPPEQVDTRNGKAQGRAPTGFSFALLPFLQAQGETEA
LRALLQRLRAMPRDDRADAYYDQVLRLFGLGWLQGRFRFERNGKLLPAWEGKCSASLPVSR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=378, Percent_Identity=50.2645502645503, Blast_Score=353, Evalue=8e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41844; Mature: 41713

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVSIPLFAALLLDALPALAAPCDGWPDWQAFLKNYVSGDGRVVDRSQDARPTTSEGQS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
YALFFALAANDRPAFDRLLAWTRDNLADGDLGARLPAWLWGRGQDGGWKVLDDNSASDSD
EEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCCCC
MWLAYDLLEAGRLWREPSYAAQGRQLALRILREETADLPGLGLTLLPGKDGFLFSDGGAR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCC
LNPSYLPPQLLARFAYGLPDSDWAKLPSGSERVLLDGAPAGFAPDWLRLQPGKGLQPDAD
CCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCC
SKAKGGYSAIRVYLWAGMLAPSAPLRAALLARYKPMARWVAREGAPPEQVDTRNGKAQGR
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
APTGFSFALLPFLQAQGETEALRALLQRLRAMPRDDRADAYYDQVLRLFGLGWLQGRFRF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCEEEE
ERNGKLLPAWEGKCSASLPVSR
CCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SAVSIPLFAALLLDALPALAAPCDGWPDWQAFLKNYVSGDGRVVDRSQDARPTTSEGQS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
YALFFALAANDRPAFDRLLAWTRDNLADGDLGARLPAWLWGRGQDGGWKVLDDNSASDSD
EEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCCCC
MWLAYDLLEAGRLWREPSYAAQGRQLALRILREETADLPGLGLTLLPGKDGFLFSDGGAR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCC
LNPSYLPPQLLARFAYGLPDSDWAKLPSGSERVLLDGAPAGFAPDWLRLQPGKGLQPDAD
CCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCC
SKAKGGYSAIRVYLWAGMLAPSAPLRAALLARYKPMARWVAREGAPPEQVDTRNGKAQGR
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
APTGFSFALLPFLQAQGETEALRALLQRLRAMPRDDRADAYYDQVLRLFGLGWLQGRFRF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCEEEE
ERNGKLLPAWEGKCSASLPVSR
CCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]