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The map label for this gene is spaS [H]

Identifier: 34498076

GI number: 34498076

Start: 2829334

End: 2830395

Strand: Reverse

Name: spaS [H]

Synonym: CV_2621

Alternate gene names: 34498076

Gene position: 2830395-2829334 (Counterclockwise)

Preceding gene: 34498077

Following gene: 34498075

Centisome position: 59.57

GC content: 63.75

Gene sequence:

>1062_bases
ATGTCGTCCAACAAGACCGAGAAACCCACGCGCAAGAAGCTGCAGGACGCGGCCAAAAAGGGCCAGTCCTTCAAGAGCCG
CGACCTGGTGGTGGCCTGCCTGACGCTGTGCGGCGTGGCTTACCTGGTGTCGTTCGGCTCGCTGGTCGAGTTGATGGGCA
TTTTCCGCCAGGCCCTGGCCGGCGGTTTCCAGCTGGACATGCGCGGCTACGCCCAGGCGGTGTTCTGGCAAGGGCTGAAA
TTGCTGCTGCCCATCTTCCTGCTGTGCGTGGCCGCCTCGGCGCTGCCGGTGTTGCTGCAGACCGGCTTCGTGCTGGCCAG
CGAGGCGCTCAAGCTCAATCTGGAGGCGCTCAACCCGATCAACGGCTTCAAGAAGCTGTTCAGCCTGCGCACGGTGAAGG
AGGCGGTGAAGGCGCTGCTGTACCTGGCCAGCTTCGTGGTGGCCGTGGCGCTGATCTGGCGCAAGCACAAGGCGCTGCTG
TTCGCCCAGCTTAACGGCAGCGTCATGGACATCGCCGCCGTGTGGCGAGAGCTGCTGCTGTCTCTGGTGCTGACCTGCCT
GGGCTGCATCGTGCTGATCCTGATTCTGGACGCGCTGGCCGAGTACTTCCTGTTCATGAAGGACATGAAGATGGACAAAC
AGGAAGTGAAGCGCGAGATGAAGGAGCAGGAAGGCAATCCCGAAATCAAGTCGCGCCGGCGCGAGGCGCATTTCGAGCTG
CTGTCCGAGCAGGTGAAGTCGGACGTGGAGAACTCCAGGCTGATCATCGCCAACCCCACCCATATCGCCATCGGCATTTA
TTTCCGGCCCGAGCTGGTGCCCATCCCCTTCGTGTCGGTGATGGAAACCAACCAGCGCGCGCTGGCGGTGCGCGCCTACG
CGGAGAAGGTCGGCGTGCCGGTGGTGCGCGACGTGCCGCTGGCGCGGCGCATCCTGGCCAGCCACCGCCGCTATTCCTTC
ATCAACCTGGAAGAGGTGGACGAGGTGCTGCGCCTGCTCGAATGGCTGGAACAGGTGGAAAACGCCGGTCGTCCGGACGA
TGAGCCGTCCGGCGGGCCATGA

Upstream 100 bases:

>100_bases
CTGCTGATCATGCTGCTCTATTTCGCGCCGGTGCTGCCCGACGCGGTGGCCGGCCTGGCCCTGCGCCCCGGCGCGCTGGG
CACCTGGTTGGTGCGCTGAC

Downstream 100 bases:

>100_bases
GCGATTCCTGAATGTTTGACCCCGGCGGGCGCGGTTAAATGACGCTGTCCAGGCTGAAAGGCCGGGACGCCAGCCAGCTC
TGCCGGGTTGGTGACCTTAC

Product: surface presentation of antigens protein SpaS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSSNKTEKPTRKKLQDAAKKGQSFKSRDLVVACLTLCGVAYLVSFGSLVELMGIFRQALAGGFQLDMRGYAQAVFWQGLK
LLLPIFLLCVAASALPVLLQTGFVLASEALKLNLEALNPINGFKKLFSLRTVKEAVKALLYLASFVVAVALIWRKHKALL
FAQLNGSVMDIAAVWRELLLSLVLTCLGCIVLILILDALAEYFLFMKDMKMDKQEVKREMKEQEGNPEIKSRRREAHFEL
LSEQVKSDVENSRLIIANPTHIAIGIYFRPELVPIPFVSVMETNQRALAVRAYAEKVGVPVVRDVPLARRILASHRRYSF
INLEEVDEVLRLLEWLEQVENAGRPDDEPSGGP

Sequences:

>Translated_353_residues
MSSNKTEKPTRKKLQDAAKKGQSFKSRDLVVACLTLCGVAYLVSFGSLVELMGIFRQALAGGFQLDMRGYAQAVFWQGLK
LLLPIFLLCVAASALPVLLQTGFVLASEALKLNLEALNPINGFKKLFSLRTVKEAVKALLYLASFVVAVALIWRKHKALL
FAQLNGSVMDIAAVWRELLLSLVLTCLGCIVLILILDALAEYFLFMKDMKMDKQEVKREMKEQEGNPEIKSRRREAHFEL
LSEQVKSDVENSRLIIANPTHIAIGIYFRPELVPIPFVSVMETNQRALAVRAYAEKVGVPVVRDVPLARRILASHRRYSF
INLEEVDEVLRLLEWLEQVENAGRPDDEPSGGP
>Mature_352_residues
SSNKTEKPTRKKLQDAAKKGQSFKSRDLVVACLTLCGVAYLVSFGSLVELMGIFRQALAGGFQLDMRGYAQAVFWQGLKL
LLPIFLLCVAASALPVLLQTGFVLASEALKLNLEALNPINGFKKLFSLRTVKEAVKALLYLASFVVAVALIWRKHKALLF
AQLNGSVMDIAAVWRELLLSLVLTCLGCIVLILILDALAEYFLFMKDMKMDKQEVKREMKEQEGNPEIKSRRREAHFELL
SEQVKSDVENSRLIIANPTHIAIGIYFRPELVPIPFVSVMETNQRALAVRAYAEKVGVPVVRDVPLARRILASHRRYSFI
NLEEVDEVLRLLEWLEQVENAGRPDDEPSGGP

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=319, Percent_Identity=25.705329153605, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 39539; Mature: 39408

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNKTEKPTRKKLQDAAKKGQSFKSRDLVVACLTLCGVAYLVSFGSLVELMGIFRQALA
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFQLDMRGYAQAVFWQGLKLLLPIFLLCVAASALPVLLQTGFVLASEALKLNLEALNPI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
NGFKKLFSLRTVKEAVKALLYLASFVVAVALIWRKHKALLFAQLNGSVMDIAAVWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
SLVLTCLGCIVLILILDALAEYFLFMKDMKMDKQEVKREMKEQEGNPEIKSRRREAHFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDVENSRLIIANPTHIAIGIYFRPELVPIPFVSVMETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCC
VVRDVPLARRILASHRRYSFINLEEVDEVLRLLEWLEQVENAGRPDDEPSGGP
CHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSNKTEKPTRKKLQDAAKKGQSFKSRDLVVACLTLCGVAYLVSFGSLVELMGIFRQALA
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFQLDMRGYAQAVFWQGLKLLLPIFLLCVAASALPVLLQTGFVLASEALKLNLEALNPI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
NGFKKLFSLRTVKEAVKALLYLASFVVAVALIWRKHKALLFAQLNGSVMDIAAVWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
SLVLTCLGCIVLILILDALAEYFLFMKDMKMDKQEVKREMKEQEGNPEIKSRRREAHFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDVENSRLIIANPTHIAIGIYFRPELVPIPFVSVMETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCC
VVRDVPLARRILASHRRYSFINLEEVDEVLRLLEWLEQVENAGRPDDEPSGGP
CHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609 [H]