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The map label for this gene is LipA

Identifier: 33863347

GI number: 33863347

Start: 1162131

End: 1163051

Strand: Reverse

Name: LipA

Synonym: PMT1076

Alternate gene names: 33863347

Gene position: 1163051-1162131 (Counterclockwise)

Preceding gene: 33863348

Following gene: 33863344

Centisome position: 48.24

GC content: 52.99

Gene sequence:

>921_bases
ATGTCTTCCACCCATCGCCCAACCCAATACAGCTCCATTTTGCCTGCGGAGCGCCTTCCGGAATGGTTGCGGCGCCCCAT
CGGCAGCGTCTCCCAATTGGAGCAGATGCAGCAGCTGGTGAAGGGGAATCATCTTCACACCATTTGTGAGGAAGGGCGTT
GTCCCAATCGCGGCGAGTGTTACGCAGCCGGTACAGCAACTTTTCTCCTAGGCGGATCGATCTGTACGCGTAGTTGTGCT
TTTTGCCAGGTCATGAAAGGCCAATCTCCTCAGGCAATCGATCCACTCGAAGCAGAGCGAGTTGCAGATGCGGTGCAGCA
GATGGGTCTTCGTTATGTGGTTCTTACTTCAGTAGCAAGGGATGATTTGCCCGACCATGGCGTCAGTATTTTTACTGAGA
CCATGGCGGCCATTCGCCAGCGCAATCCTCTTATTGAGATAGAAGTGCTGACGCCGGATTTTTGGGGCGGAGTCGCTGAT
CTGGCCAAGGCATTAGAGGCCCAGAGAGAACGACTCACGCAGTTGCTCATGGCTGCACCAGTTTGTTTCAACCACAACCT
CGAAACCGTTGAAAGGCTTCAGAGCAAGGTTCGACGGGGAGCTACGTATCACCATTCTCTCAGTCTGCTGGCTGCAGCGC
GGGAACTTGCTCCCAACATCCCTACTAAATCAGGCTTGATGTTGGGACTAGGAGAAGAGCAAGAAGAGGTGGTTCAGACT
CTTGAGGATCTCCGTTCTGTTGACTGTCAACGGGTCACATTGGGTCAGTATCTGCGCCCTTCATTGGCACATATTCCGGT
GCATCGTTATTGGCATCCTGAGGATTTCAAGAACTTGGCTGAAGTTGCTTGCAAGCTTGGATTTGCTCAGGTGCGTAGCG
GGCCTCTAGTGCGTAGTAGTTATCACGCTGGTGAAGAATGA

Upstream 100 bases:

>100_bases
CGAATCGCCTACGGCCTTCCTGTTGGTAGTGAGTTGGAGTATGCCGACGAAGTTACCCTGACTCGTGCGCTTGAAGGTCG
TCGAGCTATGCAATAAAGCA

Downstream 100 bases:

>100_bases
TCTCTGCGCAATCCCCGGCAGGTTTGCCAACGACGCTGATTACGTTCCAGCACCTCACTGCAGCTTCCAACCATTAACGC
TCCGGCTCCCCCCCAGATCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSTHRPTQYSSILPAERLPEWLRRPIGSVSQLEQMQQLVKGNHLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSCA
FCQVMKGQSPQAIDPLEAERVADAVQQMGLRYVVLTSVARDDLPDHGVSIFTETMAAIRQRNPLIEIEVLTPDFWGGVAD
LAKALEAQRERLTQLLMAAPVCFNHNLETVERLQSKVRRGATYHHSLSLLAAARELAPNIPTKSGLMLGLGEEQEEVVQT
LEDLRSVDCQRVTLGQYLRPSLAHIPVHRYWHPEDFKNLAEVACKLGFAQVRSGPLVRSSYHAGEE

Sequences:

>Translated_306_residues
MSSTHRPTQYSSILPAERLPEWLRRPIGSVSQLEQMQQLVKGNHLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSCA
FCQVMKGQSPQAIDPLEAERVADAVQQMGLRYVVLTSVARDDLPDHGVSIFTETMAAIRQRNPLIEIEVLTPDFWGGVAD
LAKALEAQRERLTQLLMAAPVCFNHNLETVERLQSKVRRGATYHHSLSLLAAARELAPNIPTKSGLMLGLGEEQEEVVQT
LEDLRSVDCQRVTLGQYLRPSLAHIPVHRYWHPEDFKNLAEVACKLGFAQVRSGPLVRSSYHAGEE
>Mature_305_residues
SSTHRPTQYSSILPAERLPEWLRRPIGSVSQLEQMQQLVKGNHLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSCAF
CQVMKGQSPQAIDPLEAERVADAVQQMGLRYVVLTSVARDDLPDHGVSIFTETMAAIRQRNPLIEIEVLTPDFWGGVADL
AKALEAQRERLTQLLMAAPVCFNHNLETVERLQSKVRRGATYHHSLSLLAAARELAPNIPTKSGLMLGLGEEQEEVVQTL
EDLRSVDCQRVTLGQYLRPSLAHIPVHRYWHPEDFKNLAEVACKLGFAQVRSGPLVRSSYHAGEE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=43.1972789115646, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=41.2451361867704, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1786846, Length=288, Percent_Identity=45.4861111111111, Blast_Score=254, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI32564533, Length=297, Percent_Identity=41.7508417508418, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=43.4343434343434, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI221513272, Length=296, Percent_Identity=43.9189189189189, Blast_Score=228, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA2_PROMM (Q7V6S0)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894907.1
- ProteinModelPortal:   Q7V6S0
- STRING:   Q7V6S0
- GeneID:   1729074
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1076
- NMPDR:   fig|74547.1.peg.1074
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   KTKIPMG
- ProtClustDB:   PRK12928
- BioCyc:   PMAR74547:PMT1076-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34003; Mature: 33872

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTHRPTQYSSILPAERLPEWLRRPIGSVSQLEQMQQLVKGNHLHTICEEGRCPNRGEC
CCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCE
YAAGTATFLLGGSICTRSCAFCQVMKGQSPQAIDPLEAERVADAVQQMGLRYVVLTSVAR
EECCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
DDLPDHGVSIFTETMAAIRQRNPLIEIEVLTPDFWGGVADLAKALEAQRERLTQLLMAAP
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VCFNHNLETVERLQSKVRRGATYHHSLSLLAAARELAPNIPTKSGLMLGLGEEQEEVVQT
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
LEDLRSVDCQRVTLGQYLRPSLAHIPVHRYWHPEDFKNLAEVACKLGFAQVRSGPLVRSS
HHHHHHCCHHHHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YHAGEE
CCCCCC
>Mature Secondary Structure 
SSTHRPTQYSSILPAERLPEWLRRPIGSVSQLEQMQQLVKGNHLHTICEEGRCPNRGEC
CCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCE
YAAGTATFLLGGSICTRSCAFCQVMKGQSPQAIDPLEAERVADAVQQMGLRYVVLTSVAR
EECCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
DDLPDHGVSIFTETMAAIRQRNPLIEIEVLTPDFWGGVADLAKALEAQRERLTQLLMAAP
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VCFNHNLETVERLQSKVRRGATYHHSLSLLAAARELAPNIPTKSGLMLGLGEEQEEVVQT
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
LEDLRSVDCQRVTLGQYLRPSLAHIPVHRYWHPEDFKNLAEVACKLGFAQVRSGPLVRSS
HHHHHHCCHHHHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YHAGEE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642