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The map label for this gene is yibK [C]

Identifier: 33863022

GI number: 33863022

Start: 818293

End: 818793

Strand: Direct

Name: yibK [C]

Synonym: PMT0750

Alternate gene names: 33863022

Gene position: 818293-818793 (Clockwise)

Preceding gene: 33863021

Following gene: 33863023

Centisome position: 33.94

GC content: 51.5

Gene sequence:

>501_bases
ATGAACGATCCAAATAAACGTTCACCATTACGCGTAGCTCTATACGAGCCACGTATTCCACCAAATACAGGAAATATCGC
CAGAACCTGTGCAGCCTTCCGCATTCCACTAGCCCTGATTGAACCTCTTGGGTTCAGCCTTGATGATCGCTATCTACGTA
GAGCAGGACTTGATTACTGGCCTCACGTCGATTTATCAGTTCATTCTGACCTGGATACATTTCGCAAGTGCCTGCCATTA
AATCATCGACTGATCGGTTGCAGCAGATTTGGAGGAATCCCCCTCCGTTCGATGCGTTTCCAAACCGGAGATGTTTTGCT
TTTCGGCCGTGAGGACACCGGTCTTCCTAGCAAAGTCCGTCAGCAGTGTGATCAGATCACGACAATTCCCATGCCTGGAG
CAGCAAGTGAACAGGGGCAGGGCGGAGTAAGGAGCCTAAACCTGTCTGTGGCTTGCGCCATCGTGAGCTTTGAGGCTGGC
CGGCAACTGGGGCTGTGGTGA

Upstream 100 bases:

>100_bases
TTGGGAAGCTATCTCAGGAACTGGAACCGTTGGCATCAAACAGCTGGCTTGTCATACAGGGTCGAGCATTGAATCTCAAA
CAACTTGGTATTGCGGTGCC

Downstream 100 bases:

>100_bases
GTAAACCGAACCAAACTTGCTTCTGCCCCCCCTTGCCTAATCGAAGGGAGATCAGCTAACTTCTCCCGGACTCCGGCCAA
ATTCGGCTTGATAGGGGTTT

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MNDPNKRSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVDLSVHSDLDTFRKCLPL
NHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPSKVRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAG
RQLGLW

Sequences:

>Translated_166_residues
MNDPNKRSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVDLSVHSDLDTFRKCLPL
NHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPSKVRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAG
RQLGLW
>Mature_166_residues
MNDPNKRSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVDLSVHSDLDTFRKCLPL
NHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPSKVRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAG
RQLGLW

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=41.4012738853503, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18402; Mature: 18402

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPNKRSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYW
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
PHVDLSVHSDLDTFRKCLPLNHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPSKVR
CCCCEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCHHHH
QQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAGRQLGLW
HHHHHEECCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MNDPNKRSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYW
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
PHVDLSVHSDLDTFRKCLPLNHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPSKVR
CCCCEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCHHHH
QQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAGRQLGLW
HHHHHEECCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]