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The map label for this gene is metG [H]

Identifier: 33863015

GI number: 33863015

Start: 812071

End: 813618

Strand: Direct

Name: metG [H]

Synonym: PMT0743

Alternate gene names: 33863015

Gene position: 812071-813618 (Clockwise)

Preceding gene: 33863013

Following gene: 33863016

Centisome position: 33.68

GC content: 44.9

Gene sequence:

>1548_bases
ATGATGACCTATACCATTACGACCCCTCTTTACTACGTCAATGACAAGCCCCATCTAGGCAGCCTGTACACAACCATGGC
CAGTGATGCACTGGCTCGTTTCCAGAGACTAGAAGGTAAGCAGGTCATGTTTGTTACAGGTGTCGATGAACACGGCCAGA
AGATTCAACGCACAGCAGAAGGCAATAACATCAGCCCCAAACATCATTGTGATCAGATCACAAGGAGTTATCAAAATCTA
TGGTCTCGTTGGGGTATTTCAAATGATCGCTTCATTCGTACAACCAGCCACCGCCATCAGTTACTAGTAGAGGCTTTTTT
TCACCGTGTCGAGACCTCCGGCGATGTATATCTTGGTCGGCAGCAGGGTTGGTATTGCGTCGGTTGTGAAGAATACAAGG
ACGAACCTCCACAAGCCCAGTCACCATGTTGCCCTATTCACCAGAAGCCTCTTGAATGGAGGGATGAGGAGAATCTTTTT
TTCAAACTCTCTCGTTTCCAATCAAAAATCGAAGATCTCATTTCGCAGCCGGATTTCATCTGGCCAGTCATGCGCCGCAA
TGAAATACAGAATTTTGTAGCTCAGGGTCTTAGGGATTTTTCAATTTCAAGAGTTGATGTTGATTGGGGCCTACCTGTAC
CTGGTTATCCAAAACACACTTTTTATGTCTGGTTTGATGCGCTTGTAGGCTATTTGAGTGCTCTTTTAGATGATGGTGGC
CAGGTTCGAATAGAACGTCTTGAGGAGTGCGGATGGCCTGCATCAGTACATTTAATTGGCAAGGATATCCTCCGTTTTCA
TGCAGTCTACTGGCCCGCAATGCTGATCTCAGCTGGCTTACCCTTGCCAAAACAAGTTTTTGGCCACGGCTTTCTCACTC
GTGAAGGTCAAAAGATGGGTAAATCACTAGGAAATGTTCTTGATCCAGAGAAACTGCTAGAAATATGTGGTGCAGATGCT
GTTCGATGGTATTTATTGCGTGACATCCAGTTCGGTCATGATGGTGACTTTCAGCAGAAAAGATTTATGGATTTAGTTAA
TAACGACTTGGCAAATACAATCGGAAATCTGCTGAACCGAACTTCGACTATGGCAAGAAAATGGTTTGAAGGATCTGTCC
CTACACATCGCGATGAAATAGATTACGCTCATCCCTTAGTGACCACTGCTGAAACAACAATTACCATCGTACGTCAATCA
ATGCCATCCATGGCTTTTCATGATGCAGCTGAGGCGATCCTTAAATTGGCTATAGCAGCCAACGTCTACCTCAACGAAAC
TGCACCTTGGAGTCGAATGAAGACGCCTGGCAATGAAGCCGAAGTGGCTGATGATCTTTACTCAGTTTTGGAAGCATGCC
GACTTGTAGGACTATTAATTAATCCTCTTGTACCTTCTCTAAGCGAACGCTTGCTCAACCAATTGGGATTAAGTATTGAT
GGCAACGATTGGTCTAAAGGTTTAGCTTGGGGACAATTGCAAAGTGGAGCTTTATTGCCACCATCTCAACCCGTAATGCA
AAGACTAGAACTCTCTTCTTCCCTCTAA

Upstream 100 bases:

>100_bases
GTCGGCTTGGTCTGAGAGATGACGGCAGACGATCAATAAGCTGAAATGAGTTGTCTGGTTATGATCCGGCCGCTCTGCGC
AGCAGCAGAAACTCCCCAAA

Downstream 100 bases:

>100_bases
TGCAATTCAACCTTCTACCATTACGTCATCTTGGCAGTCTGCTGCTGATCATAACGATGGTGGGATGTCAGGAATCGCCA
TCACCACAGAAGAAATCATC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEGNNISPKHHCDQITRSYQNL
WSRWGISNDRFIRTTSHRHQLLVEAFFHRVETSGDVYLGRQQGWYCVGCEEYKDEPPQAQSPCCPIHQKPLEWRDEENLF
FKLSRFQSKIEDLISQPDFIWPVMRRNEIQNFVAQGLRDFSISRVDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLEECGWPASVHLIGKDILRFHAVYWPAMLISAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLLEICGADA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPTHRDEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
GNDWSKGLAWGQLQSGALLPPSQPVMQRLELSSSL

Sequences:

>Translated_515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEGNNISPKHHCDQITRSYQNL
WSRWGISNDRFIRTTSHRHQLLVEAFFHRVETSGDVYLGRQQGWYCVGCEEYKDEPPQAQSPCCPIHQKPLEWRDEENLF
FKLSRFQSKIEDLISQPDFIWPVMRRNEIQNFVAQGLRDFSISRVDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLEECGWPASVHLIGKDILRFHAVYWPAMLISAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLLEICGADA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPTHRDEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
GNDWSKGLAWGQLQSGALLPPSQPVMQRLELSSSL
>Mature_515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEGNNISPKHHCDQITRSYQNL
WSRWGISNDRFIRTTSHRHQLLVEAFFHRVETSGDVYLGRQQGWYCVGCEEYKDEPPQAQSPCCPIHQKPLEWRDEENLF
FKLSRFQSKIEDLISQPDFIWPVMRRNEIQNFVAQGLRDFSISRVDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLEECGWPASVHLIGKDILRFHAVYWPAMLISAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLLEICGADA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPTHRDEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
GNDWSKGLAWGQLQSGALLPPSQPVMQRLELSSSL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=504, Percent_Identity=35.515873015873, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI14043022, Length=551, Percent_Identity=25.2268602540835, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788432, Length=500, Percent_Identity=26.8, Blast_Score=188, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI212645096, Length=494, Percent_Identity=31.3765182186235, Blast_Score=255, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71992781, Length=389, Percent_Identity=30.0771208226221, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17540794, Length=524, Percent_Identity=26.5267175572519, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321610, Length=524, Percent_Identity=33.3969465648855, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=520, Percent_Identity=23.2692307692308, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358483, Length=505, Percent_Identity=32.4752475247525, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=25.5855855855856, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58538; Mature: 58538

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAE
CEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
GNNISPKHHCDQITRSYQNLWSRWGISNDRFIRTTSHRHQLLVEAFFHRVETSGDVYLGR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEC
QQGWYCVGCEEYKDEPPQAQSPCCPIHQKPLEWRDEENLFFKLSRFQSKIEDLISQPDFI
CCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
WPVMRRNEIQNFVAQGLRDFSISRVDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVRIERLEECGWPASVHLIGKDILRFHAVYWPAMLISAGLPLPKQVFGHGFLTREGQKMG
CCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHH
KSLGNVLDPEKLLEICGADAVRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNR
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TSTMARKWFEGSVPTHRDEIDYAHPLVTTAETTITIVRQSMPSMAFHDAAEAILKLAIAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
HEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GNDWSKGLAWGQLQSGALLPPSQPVMQRLELSSSL
CCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAE
CEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
GNNISPKHHCDQITRSYQNLWSRWGISNDRFIRTTSHRHQLLVEAFFHRVETSGDVYLGR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEC
QQGWYCVGCEEYKDEPPQAQSPCCPIHQKPLEWRDEENLFFKLSRFQSKIEDLISQPDFI
CCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
WPVMRRNEIQNFVAQGLRDFSISRVDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVRIERLEECGWPASVHLIGKDILRFHAVYWPAMLISAGLPLPKQVFGHGFLTREGQKMG
CCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHH
KSLGNVLDPEKLLEICGADAVRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNR
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TSTMARKWFEGSVPTHRDEIDYAHPLVTTAETTITIVRQSMPSMAFHDAAEAILKLAIAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
HEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GNDWSKGLAWGQLQSGALLPPSQPVMQRLELSSSL
CCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]