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The map label for this gene is pykF [H]

Identifier: 33862951

GI number: 33862951

Start: 735528

End: 737345

Strand: Reverse

Name: pykF [H]

Synonym: PMT0679

Alternate gene names: 33862951

Gene position: 737345-735528 (Counterclockwise)

Preceding gene: 33862954

Following gene: 33862950

Centisome position: 30.58

GC content: 50.77

Gene sequence:

>1818_bases
ATGGTAGTTTCCGCGATGTTTCAGGTCCCGTTGATGGCCCAGATTGATCTGACACGACGGACCAAAATTGTTGCCACCAT
AGGTCCGGCAACCGAGAGCGCCGAACGAATCCGAGAACTCGTTGAGGTAGGTGCTACCACCTTCCGGCTGAATTTCTCAC
ACGGCGACCATGACGAGCACGCCAAGCGCATCTCCACCATCCGTCAGGTTTCTGATGAATTGGGAATGCACATCGGCATT
CTTCAAGACTTGCAAGGCCCCAAAATTCGCCTGGGTCGTTTTGATAAGGGGCCTATCACTCTGGCCAATGGCGATTCCTT
CGCACTGACCTCCAAACAAGTTCAATGCAATCAGTCAGTGGCAACCGTCACTTACGACAAGCTTGCAGACGAAGTCACGA
CCGGCAGCCGAATCTTGCTTGATGACGGTCGAGTGGAGATGAAAGTCTTAAAAATCGATCAAAGCGAACAAACCTTGCAC
TGCATCGTCACGGTGGGAGGGGTGCTCTCAAATAACAAGGGAGTGAATTTCCCTGATGTACAGCTTTCGGTTCGCGCCCT
TACAGAGAAAGATCGCCAAGATCTCGCCTTTGGCCTCCAGCAAGGGGTGGACTGGGTCGCACTGAGCTTTGTGCGAAACC
CTTCGGACATGAAAGAGATCCGCAAACTCATCCGCGATCATGGGCACAGCACCCCAGTGATCGCGAAGATCGAGAAATTT
GAGGCGATTGATCAAATCGATTCAATCCTTCCTCTCTGTGATGGAGTCATGGTTGCAAGGGGGGACTTAGGCGTTGAGAT
GCCAGCCGAAGAAGTGCCTCTACTGCAAAAGGATTTGATTCGCAAGGCCAACAGCCTTGGAATTCCAATCATCACAGCCA
CCCAGATGCTCGACTCGATGGCCTCAAGCCCGAGGCCCACCAGAGCAGAAGTCAGTGATGTAGCAAATGCGATCCTTGAT
GGTACTGATGCAGTGATGCTCTCGAACGAAACCGCTGTAGGAGACTATCCAGTGGAGGCGGTTCAAACGATGGCCACCAT
TGCCAGACGGATTGAACGGGACTATCCACAGCGTCCAATCGAAACTTATCTAGCCAGCACCATTTCCAACGCGATTAGTT
CAGCCGTAAGCAGCATCGCCAGTCATCTCAACGCGGCAGCAATCGTGCCACTAACTGAAAGTGGTGCAACTGCTCACAAC
GTCAGTAAATTCCGCCCAGCAGCGCCAATTTTGGCCGTAACCAACAAATTGGCTGTAGCAAGAAAACTTCAATTGGCATG
GGGTGTTACCCCACTGCTTGTCCCAACCCAAAAAAGTACCACTGCAACCTTCAGTGATGCAATTGCTGTTGCCCAGGAAT
TGGGTCTTCTCAAAGAAGGAGATCTAACAATTCAAACTGCAGGAACCCTTGCTGGCGTGAGCGGGTCCACAGATCTTGTC
AAAGTAGGAATTGTCAGTGCAGTACTTGGCCAAGGGATCGGTATTGGCAATGGAACCGTTAGCGGCAAGGTCAGATTTGC
TTTCTCCCCAAACGACTCAACAAAAATTGAACCAGGCGAGATTCTGGTCATTCATGAAACAAGCGATGACTATCTCGATG
CAATCAGAAAGTCTGCAGGAGTGATTACAGAACAAGAAGGAGAGGAATACCACGCAGAAATGATCTCAAAACGACTAGGG
ATTCCGTTCATCATTGGGATAGCACATGCCACGAGAAACCTGCTTGAAGGAGAGGTGGTAACAATGCAGCTCAGTAATGG
CAAAGTTCATCGCGGCACAGGCGGCAAGCTGCCAATGAAGCTCGACTCAATGGACTGA

Upstream 100 bases:

>100_bases
ATTCGGATAGCGGGCCGTAGCTCGAGCTCCATGCTGGTAATGATTAATGTCCATAGAAAATCATTGGCAACGCTGTCAAT
AACAACGTTTTGAGGCCTGG

Downstream 100 bases:

>100_bases
GGGCTCATGAATAGCTCACTTCCGCTCATCGAAACGGTGAGCATGGCGTTCTCAACCCTTAAAGCCAATCGCCTACGTAG
TCTCCTGACAATGCTCGGCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATESAERIRELVEVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDSFALTSKQVQCNQSVATVTYDKLADEVTTGSRILLDDGRVEMKVLKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIRDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNKLAVARKLQLAWGVTPLLVPTQKSTTATFSDAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEEYHAEMISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSMD

Sequences:

>Translated_605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATESAERIRELVEVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDSFALTSKQVQCNQSVATVTYDKLADEVTTGSRILLDDGRVEMKVLKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIRDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNKLAVARKLQLAWGVTPLLVPTQKSTTATFSDAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEEYHAEMISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSMD
>Mature_605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATESAERIRELVEVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDSFALTSKQVQCNQSVATVTYDKLADEVTTGSRILLDDGRVEMKVLKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIRDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNKLAVARKLQLAWGVTPLLVPTQKSTTATFSDAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEEYHAEMISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSMD

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=40.081799591002, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=40.081799591002, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=40.2040816326531, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=40.2040816326531, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI33286418, Length=502, Percent_Identity=39.0438247011952, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=38.2758620689655, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=38.2758620689655, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=34.453781512605, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=34.453781512605, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=32.7188940092166, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=43.7632135306554, Blast_Score=355, Evalue=6e-99,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=41.1392405063291, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1787994, Length=100, Percent_Identity=31, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17544584, Length=429, Percent_Identity=40.7925407925408, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=40.9465020576132, Blast_Score=315, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=40.9465020576132, Blast_Score=315, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=40.9465020576132, Blast_Score=314, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=40.9465020576132, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=38.6694386694387, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=37.1428571428571, Blast_Score=283, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24648964, Length=500, Percent_Identity=38.2, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI28571814, Length=500, Percent_Identity=38.2, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=40.2439024390244, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI24581235, Length=502, Percent_Identity=34.0637450199203, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=42.0074349442379, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64894; Mature: 64894

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATESAERIRELVEVGATTFRLNFSHGDHDEH
CEEECHHCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AKRISTIRQVSDELGMHIGILQDLQGPKIRLGRFDKGPITLANGDSFALTSKQVQCNQSV
HHHHHHHHHHHHHHCCEEEHHHHCCCCCEEECCCCCCCEEEECCCCEEEECCHHCCCCCE
ATVTYDKLADEVTTGSRILLDDGRVEMKVLKIDQSEQTLHCIVTVGGVLSNNKGVNFPDV
EEEEHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEEEECCEEECCCCCCCCCC
QLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIRDHGHSTPVIAKIEKF
EEEHHHHHHHHHHHHHHHHHHCCHHEEHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSM
HHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
ASSPRPTRAEVSDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPI
HCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHNVSKFRPAAPILAVTNKLAVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHH
RKLQLAWGVTPLLVPTQKSTTATFSDAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCHHH
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAG
HHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
VITEQEGEEYHAEMISKRLGIPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMK
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCEE
LDSMD
ECCCC
>Mature Secondary Structure
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATESAERIRELVEVGATTFRLNFSHGDHDEH
CEEECHHCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AKRISTIRQVSDELGMHIGILQDLQGPKIRLGRFDKGPITLANGDSFALTSKQVQCNQSV
HHHHHHHHHHHHHHCCEEEHHHHCCCCCEEECCCCCCCEEEECCCCEEEECCHHCCCCCE
ATVTYDKLADEVTTGSRILLDDGRVEMKVLKIDQSEQTLHCIVTVGGVLSNNKGVNFPDV
EEEEHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEEEECCEEECCCCCCCCCC
QLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIRDHGHSTPVIAKIEKF
EEEHHHHHHHHHHHHHHHHHHCCHHEEHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSM
HHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
ASSPRPTRAEVSDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPI
HCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHNVSKFRPAAPILAVTNKLAVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHH
RKLQLAWGVTPLLVPTQKSTTATFSDAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCHHH
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAG
HHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
VITEQEGEEYHAEMISKRLGIPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMK
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCEE
LDSMD
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]