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The map label for this gene is miaA [H]

Identifier: 33862393

GI number: 33862393

Start: 137450

End: 138349

Strand: Reverse

Name: miaA [H]

Synonym: PMT0120

Alternate gene names: 33862393

Gene position: 138349-137450 (Counterclockwise)

Preceding gene: 33862398

Following gene: 33862392

Centisome position: 5.74

GC content: 56.67

Gene sequence:

>900_bases
ATGCCCGCCTCTAAGCCGCTTCTGATTGCGCTGCTAGGGCCCACAGCAAGCGGCAAAACATGCCTGGCCTTACAGCTGGC
AGAACAGCTGAAGCTGAGCGTGCTCAATGTGGATTCCCGCCAGCTCTACATCGGCATGGACGTTGGCACCGCCAAGCCAA
CCAAAGAACAGCAGCGGCGTGTGCAGCATCACCTGATTGATCTTCGTCGCCCAGATCAACCAATCACCCTGCAGGAATTT
CAGGCAGCCGCGCAACTGATCCTTGCGCAAAAGCTAAGGGAACAAAACATGCCATTTCTAGTTGGCGGCAGTGGTCTTTA
TCTCAAAGCTCTCACTTGTGGGCTGCGCCCACCCGCAGTGCCACCCCAGCCAGAACTCCGCAAGCAGTTAGGCGAGCTAG
GCCAACGCACCTGCCACCACCTACTTCAAGCCGCCGATCCCACCGCTGCCAACCGAATTTCCCCTGCTGATGCCATGCGC
ACTCAAAGGGGTCTGGAAGTGGTTTATGCCACTGGCAAACCGATCACCACTCAACAAGGCTCAAGCCCACCCCCTTGGCG
CGTGCTGGAACTAGGCCTCGACCCACACAACCTCCGCGAACGCATTGGCCATCGCACAACACAGCTCTATGCCAATGGTC
TCATCGAAGAAACCGAACACTTGAGTCATTGCTACGACACCGATCTGCCCTTGCTGCAAACGATTGGCTATGGGGAAGCA
CTAAAGGTGATTCAAGGCCTCCTCAACCGTGAACAGGCCATCGCCCTGACCACACGTCGTACTCAACAGTTCGCCAAGCG
TCAGCGCACATGGTTCCGCAGACAACATCACCCCTATTGGCTCAAAGGTGAGGAACCGCTGAGCGAAGCGCTATCACTGA
TTCAAGCGGGTCTAAGGTGA

Upstream 100 bases:

>100_bases
CCACTGGCTCACCACTGGCGCTTACAACGGTCACTGAAGCGTCATTGCTCAAAAGGGACGGACCTAAACGTTGCTGACAT
GACACCCATCACAGCTGCTC

Downstream 100 bases:

>100_bases
AGGGTGCAGACATAAAACCTTCTGGTCTCCTTGTCGTCTGCTCTATTCCCTTATCTCACTGAATGCCACCACGTCCTCGT
TTTGACCGTCGCGCACCCGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEF
QAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAANRISPADAMR
TQRGLEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGHRTTQLYANGLIEETEHLSHCYDTDLPLLQTIGYGEA
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR

Sequences:

>Translated_299_residues
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEF
QAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAANRISPADAMR
TQRGLEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGHRTTQLYANGLIEETEHLSHCYDTDLPLLQTIGYGEA
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
>Mature_298_residues
PASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEFQ
AAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAANRISPADAMRT
QRGLEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGHRTTQLYANGLIEETEHLSHCYDTDLPLLQTIGYGEAL
KVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=25.0793650793651, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=38.0952380952381, Blast_Score=180, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6324848, Length=308, Percent_Identity=26.6233766233766, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24649830, Length=262, Percent_Identity=31.6793893129771, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33451; Mature: 33320

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRR
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHH
VQHHLIDLRRPDQPITLQEFQAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHCCCCCCC
PPQPELRKQLGELGQRTCHHLLQAADPTAANRISPADAMRTQRGLEVVYATGKPITTQQG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCEEEEECCCEEECCCC
SSPPPWRVLELGLDPHNLRERIGHRTTQLYANGLIEETEHLSHCYDTDLPLLQTIGYGEA
CCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHH
VQHHLIDLRRPDQPITLQEFQAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHCCCCCCC
PPQPELRKQLGELGQRTCHHLLQAADPTAANRISPADAMRTQRGLEVVYATGKPITTQQG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCEEEEECCCEEECCCC
SSPPPWRVLELGLDPHNLRERIGHRTTQLYANGLIEETEHLSHCYDTDLPLLQTIGYGEA
CCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA