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The map label for this gene is gspE [H]

Identifier: 308513280

GI number: 308513280

Start: 356895

End: 358439

Strand: Reverse

Name: gspE [H]

Synonym: NA

Alternate gene names: 308513280

Gene position: 358439-356895 (Counterclockwise)

Preceding gene: 39995438

Following gene: 39995436

Centisome position: 9.4

GC content: 64.08

Gene sequence:

>1545_bases
ATGGAACAGATCGCCCGGCGGCTCGGCATCCCCTTCCTGGCCGAGATCGGGGACAATGAGGCGGATGCGGCACTGCTCGC
GCGGCTGCCCCTGGCTTTTGCCCGGGGCAGGCTCGTCCTTCCCCTGCGGGAGCGGGATGGGCGGCTCCTGGTTGTTTCAG
GCAATCCCGCGGATCTGTCGGCCATCGATGAGGTGCGGGGAGTCTACGGGATGGAGGTGGAACTGGCCGCGGCAACGCCG
GATACGGTGCTCGGCGCGGTGAACCATCTCTACGCCCGGCTCGGCAGCTCGGCCCAGGAGGTCGTGGAAGAGCTTGAAGG
GGAAGACCTTTCGGTCATCGCCACTGAGCTGGCCGAGCCCAAGGATCTTCTGGACCTGACCGACGAAGCGCCGGTCATCC
GGCTTCTGAACTCGATCCTCTCGGAGGCGGTGAAGGAGCGGGCCAGCGACATCCACATCGAACCCTACGAGCGGGAACTG
GAGGTTCGTTTCAGGATCGACGGCATCCTCTACCGCAAGCTCGCCCCTCCCAAGGTGGTGCAGGAAGCTCTGGTCTCCCG
CGTGAAGATCATGGCCGGGTTGAACATCGCCGAAAAGCGCCTTCCCCAGGATGGCCGCATCAGGGTAATCGTAGCGGGAC
GGGATGTGGACATCCGTGTCTCCATCATCCCTACCTTCTTCGGCGAACGCGTGGTGCTCCGTCTCCTGGACAAACAGAAG
GGGCTCATTTCTCTCGAAAACATCGGGCTGTCCGAAGGAGGCGTCCGCTCCATGGAGCGCCTCCTCGGCCGGACCAGCGG
CATCATCCTCGTCACCGGGCCAACGGGAAGCGGCAAGTCCACCACCCTCTACGCGGCACTCAACCGGCTCAACTCGCCGG
AAAAGAACATCATCACCATCGAAGACCCCATCGAATATCAAGTCAAGGGGATCGGCCAGATTCAGGTCAATCCCAAGATC
GAGCTGACCTTTGCCCAGGGCCTGCGCGCCATTCTCCGCCAGGACCCGGACATCGTAATGGTGGGAGAGATCCGCGACGC
CGAGACGGCGGAAATCGCCATGCAGGCGTCACTCACGGGGCACCTGGTCCTCTCTACCCTTCACACCAACGACAGTGCCA
CCGCCATCGCCCGACTCGTCGACATGGGAATCGAACCGTTCATGGTGGCATCGTCCCTGTCCGCTGTTCTGGCCCAGCGG
CTCGTGCGCCGCATCTGCCCCCACTGCCGCGAATCCTACACGCCGGAACGGGACTACGCCGGCATCACCCTCCCCTCAAC
CCTCTACCGGGGGCGGGGATGCGATGCATGCTTCGGCCTCGGGACCCTGGGCCGGGTCGGCATCTACGAACTCCTACCCG
TGGACGGGGAGATCTGCTCCATGATCATCCGTCGGGAACCGGCCGGAGCCATCAAGGAATACGCTGTCGGCAAGGGGATG
CGGACCCTGCGCGACGACGGCCTGGCCAAGGCCGCAGCGGGCATCACAACCATCGAGGAGGTCCTGCGGGTAACGCAGGA
GGAGTATGCCGACCTTCCGGTATAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:
NA

Sequences:

>Translated_514_residues
MEQIARRLGIPFLAEIGDNEADAALLARLPLAFARGRLVLPLRERDGRLLVVSGNPADLSAIDEVRGVYGMEVELAAATP
DTVLGAVNHLYARLGSSAQEVVEELEGEDLSVIATELAEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYEREL
EVRFRIDGILYRKLAPPKVVQEALVSRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERVVLRLLDKQK
GLISLENIGLSEGGVRSMERLLGRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITIEDPIEYQVKGIGQIQVNPKI
ELTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQR
LVRRICPHCRESYTPERDYAGITLPSTLYRGRGCDACFGLGTLGRVGIYELLPVDGEICSMIIRREPAGAIKEYAVGKGM
RTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
>Mature_514_residues
MEQIARRLGIPFLAEIGDNEADAALLARLPLAFARGRLVLPLRERDGRLLVVSGNPADLSAIDEVRGVYGMEVELAAATP
DTVLGAVNHLYARLGSSAQEVVEELEGEDLSVIATELAEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYEREL
EVRFRIDGILYRKLAPPKVVQEALVSRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERVVLRLLDKQK
GLISLENIGLSEGGVRSMERLLGRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITIEDPIEYQVKGIGQIQVNPKI
ELTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQR
LVRRICPHCRESYTPERDYAGITLPSTLYRGRGCDACFGLGTLGRVGIYELLPVDGEICSMIIRREPAGAIKEYAVGKGM
RTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=415, Percent_Identity=50.6024096385542, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=43.3862433862434, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87082188, Length=279, Percent_Identity=35.1254480286738, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55988; Mature: 55988

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIARRLGIPFLAEIGDNEADAALLARLPLAFARGRLVLPLRERDGRLLVVSGNPADLS
CHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHH
AIDEVRGVYGMEVELAAATPDTVLGAVNHLYARLGSSAQEVVEELEGEDLSVIATELAEP
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHCCC
KDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYERELEVRFRIDGILYRKLAPPKVV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEECCCCHHHH
QEALVSRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERVVLRLLDKQK
HHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCC
GLISLENIGLSEGGVRSMERLLGRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITI
CEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEE
EDPIEYQVKGIGQIQVNPKIELTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTG
ECCCEEEEEECEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCE
HLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQRLVRRICPHCRESYTPERDYA
EEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GITLPSTLYRGRGCDACFGLGTLGRVGIYELLPVDGEICSMIIRREPAGAIKEYAVGKGM
CCCCCHHHHCCCCCCHHHCCCCCCCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHCCCH
RTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQIARRLGIPFLAEIGDNEADAALLARLPLAFARGRLVLPLRERDGRLLVVSGNPADLS
CHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHH
AIDEVRGVYGMEVELAAATPDTVLGAVNHLYARLGSSAQEVVEELEGEDLSVIATELAEP
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHCCC
KDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYERELEVRFRIDGILYRKLAPPKVV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEECCCCHHHH
QEALVSRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERVVLRLLDKQK
HHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCC
GLISLENIGLSEGGVRSMERLLGRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITI
CEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEE
EDPIEYQVKGIGQIQVNPKIELTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTG
ECCCEEEEEECEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCE
HLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQRLVRRICPHCRESYTPERDYA
EEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GITLPSTLYRGRGCDACFGLGTLGRVGIYELLPVDGEICSMIIRREPAGAIKEYAVGKGM
CCCCCHHHHCCCCCCHHHCCCCCCCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHCCCH
RTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]