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The map label for this gene is hydN

Identifier: 30064072

GI number: 30064072

Start: 2805886

End: 2806413

Strand: Reverse

Name: hydN

Synonym: S2927

Alternate gene names: 30064072

Gene position: 2806413-2805886 (Counterclockwise)

Preceding gene: 30064073

Following gene: 30064071

Centisome position: 61.02

GC content: 52.84

Gene sequence:

>528_bases
ATGAACCGTTTCATCATTGCTGACGCGAGTAAATGTATTGGTTGCCGTACCTGTGAAGTGGCGTGCGTGGTTTCTCATCA
GGAAAATCAGGACTGTGCATCGCTGACCCCGGAAACATTTTTACCGCGTATCCATGTCATTAAAGGTGTGAACATTTCCA
CGGCGACAGTTTGCCGTCAGTGTGAAGATGCACCGTGCGCTAACGTCTGCCCGAATGGTGCTATCAGCCGTGATAAAGGG
TTTGTTCCTGTGATGCAGGAACGTTGCATTGGTTGCAAAACCTGCGTGGTGGCTTGCCCGTATGGCGCGATGGAAGTGGT
GGTACGTCCTGTGATTCGCAACAGCGGCGCGGGTCTGAATGTACGGGCTGACAAAGCCGAAGCCAATAAATGCGACCTGT
GCAACCATCGTGAAGACGGCCCGGCGTGTATGGCGGCTTGCCCGACCCATGCGCTGATTTGTGTCGATCGTAATAAACTT
GAACAACTGAGCGCAGAAAAACGCCGCCGTACGGCGCTGATGTTCTAA

Upstream 100 bases:

>100_bases
CCTGCATTACAGAAATTAATTTCCTTAAATAACAGCAAATTAAAAACTGGCATGATTTGTGAGTGTATCGGCGCATTAAC
TGTCATTGCTGGAGAATTTG

Downstream 100 bases:

>100_bases
AAATCTTACAGAACTGTTCTGGCATCAAAGGAATTCCCGGAAATATTCGTAGAATACGGAGCGGGCGGTATCGCCGACCT
GAGACTGCCGGATACGACGC

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVPVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Sequences:

>Translated_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVPVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF
>Mature_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVPVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=99.4285714285714, Blast_Score=352, Evalue=9e-99,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=61.7283950617284, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=48.8372093023256, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=40.1197604790419, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=39.3063583815029, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789079, Length=190, Percent_Identity=39.4736842105263, Blast_Score=120, Evalue=3e-29,
Organism=Escherichia coli, GI87082114, Length=199, Percent_Identity=34.6733668341709, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.7683615819209, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=33.1325301204819, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI226510944, Length=158, Percent_Identity=35.4430379746835, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=154, Percent_Identity=33.7662337662338, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787963, Length=174, Percent_Identity=28.1609195402299, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18986; Mature: 18986

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
10.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]