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The map label for this gene is intA [H]

Identifier: 30064019

GI number: 30064019

Start: 2749333

End: 2750574

Strand: Direct

Name: intA [H]

Synonym: S2859

Alternate gene names: 30064019

Gene position: 2749333-2750574 (Clockwise)

Preceding gene: 30064018

Following gene: 30064020

Centisome position: 59.78

GC content: 49.28

Gene sequence:

>1242_bases
ATGGCAAGACAAACCAAACCTCTATCCGTTAAGGAAATCGAATCTGCTAAACCCAAGGAAGCGGACTACGTTCTCTATGA
TGGCGATGGCCTTGAGCTACTCATTAAATCCAGCGGGAGTAAAATCTGGCAGTTTCGCTACATTCGCCCTGTCACCAAGA
CACGAGCAAAGAAAAGCATAGGCCCTTACCCGTCAGTTACCCTTGCCGATGCCAGAAACTACCGTGCAGAGTCCCGCTCA
CTCCTAGCTAAACAGATTGATCCGCAGGAACATCAGCAAGAACAATTGCGTAGTTCGCTGGAAGCCAAAACCAATACTTT
CCAGCTCGTAGCTGAACGTTGGTGGAATGTGAAGAAAGCCAGTGTGACAGAGGACTATGCGGAAGATATCTGGCGCTCTC
TTGAAAGAGATGTCTTTCCTGCGATTGGCGACGTTAGCGTTACAGATATTAAAGCTCATACACTGGTTCAGGCCGTACAA
CCGGTTCAGGCCAGAGGAGCACTGGAAACGGTTCGTCGCCTGTGTCAACGTATTAATGAGGTCATGATCTATGCCCAGAA
CACAGGGCTGATTGATGCTGTTCCCAGCGTTAATATCGGTAAAGCCTTCGAAAAGCCTCAGAAAAAGAACATGCCCAGCA
TTCGACCGGATCAGCTATCTCAACTGATGCAGACAATGCGAACAGCCAGCATTAGCCTTTCCACACGCTGCCTGTTCATG
TGGCAACTTCTTACTATTACCCGCCCTGCCGAAGCGGCTGAAGCTCGCTGGGAAGAAGTAGACATAGAAGCGCAAGAGTG
GAAGATTCCCGCAGCACGCATGAAAATGAACCGCGACCATACTGTTCCATTGTCAGATGAAGCAATAGCTGTGCTGGAGA
TGATGAAACCGCTAAGCGGCAACCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAGCCTATGAACAGTCAGACT
GTTAACGCCTCACTAAAACGCGCAGGTTTTGGTGGTGTGCTTGTTTCGCATGGTCTGAGATCAATTGCCAGTACGGCTCT
TAATGAACAAGGCTTTCCGCCTGATGCCATTGAGGCAGCATTGGCCCATGTGGATAAGAATGAGGTTCGTCGTGCTTATA
ACCGCAGCGATTACCTGGAACAGCGTCGCCCGATGATGCAATGGTGGGCAAATTTTGTGATGGCAGCGGATCGTGGAAGC
ATGATTGAAGGTGGGATAAAAGGAATGAAGCTAGTTGGGTGA

Upstream 100 bases:

>100_bases
CTGATGCCAACTAATTAAATCCGATCCTTTTAGGCACCTTGTTAGGCACTTCATAAAGCTATATTGTCTTTGAGGTGCCT
AAAACTATGGAAACCCGGCA

Downstream 100 bases:

>100_bases
CTAAATAGCTGCGCGGCATAGTGTCCCACTTTCAGCCAGCCCTTGCAGGTTAAGCCACCAGACAGTAAACGGCGGGTGTC
GCGTTATTAGGCGCAGCTAT

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQFRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRS
LLAKQIDPQEHQQEQLRSSLEAKTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLSQLMQTMRTASISLSTRCLFM
WQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKMNRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQT
VNASLKRAGFGGVLVSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFVMAADRGS
MIEGGIKGMKLVG

Sequences:

>Translated_413_residues
MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQFRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRS
LLAKQIDPQEHQQEQLRSSLEAKTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLSQLMQTMRTASISLSTRCLFM
WQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKMNRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQT
VNASLKRAGFGGVLVSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFVMAADRGS
MIEGGIKGMKLVG
>Mature_412_residues
ARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQFRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSL
LAKQIDPQEHQQEQLRSSLEAKTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQP
VQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLSQLMQTMRTASISLSTRCLFMW
QLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKMNRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTV
NASLKRAGFGGVLVSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFVMAADRGSM
IEGGIKGMKLVG

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=413, Percent_Identity=83.7772397094431, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1788690, Length=390, Percent_Identity=33.0769230769231, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI145693166, Length=403, Percent_Identity=34.4913151364764, Blast_Score=215, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46654; Mature: 46523

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQFRYIRPVTKTRAKKSI
CCCCCCCCCHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEEECHHHHHHHHHCC
GPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEAKTNTFQLVAERWWNVKKA
CCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQPVQARGALETVRRLCQRINE
HHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLSQLMQTMRTASISLSTRCLFM
EEEEECCCCCEECCCCCCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKMNRDHTVPLSDEAIAVLEMMKPLSG
HHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
NREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVLVSHGLRSIASTALNEQGFPPDAIEAA
CCCEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFVMAADRGSMIEGGIKGMKLVG
HHHCCHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHCCCCCCEECC
>Mature Secondary Structure 
ARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQFRYIRPVTKTRAKKSI
CCCCCCCCHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEEECHHHHHHHHHCC
GPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEAKTNTFQLVAERWWNVKKA
CCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQPVQARGALETVRRLCQRINE
HHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLSQLMQTMRTASISLSTRCLFM
EEEEECCCCCEECCCCCCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKMNRDHTVPLSDEAIAVLEMMKPLSG
HHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
NREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVLVSHGLRSIASTALNEQGFPPDAIEAA
CCCEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFVMAADRGSMIEGGIKGMKLVG
HHHCCHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]